A5005450540- Treatment of Major Depression
- Neurotransmitter Receptor Influence on Behavior
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Stress Responses and Cortisol
- Analytical Chemistry and Sensors
- Tryptophan and brain disorders
University of Bremen
2022-2024
We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT are bright in unbound state diminish their fluorescence upon binding 5-HT. Sensor variants with different affinities for were engineered to increase versatility imaging dynamics. Experiments vitro vivo showed feasibility dynamics high temporal spatial resolution. As demonstrated here, designed show excellent membrane expression, have...
Abstract Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio red-shifted posed significant challenges. More critically, several red-fluorescent exhibit photoswitching when exposed to blue light, thereby limiting applicability in all-optical experimental approaches. Here, we present the...
Abstract We developed a new family of genetically encoded serotonin (5-HT) sensors ( sDarken ) on the basis native 5-HT 1A receptor and circularly permuted GFP. are bright in unbound state diminish their fluorescence upon binding 5-HT. Sensor variants with different affinities for were engineered to increase versatility imaging dynamics. Experiments vitro vivo showed feasibility dynamics high temporal spatial resolution. As demonstrated here, designed excellent membrane expression, have...