A5015494472- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Neurotransmitter Receptor Influence on Behavior
- Photoreceptor and optogenetics research
- Graphene and Nanomaterials Applications
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Diet and metabolism studies
- Carbon Nanotubes in Composites
- Advanced Chemical Sensor Technologies
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Sensors
- Memory and Neural Mechanisms
- Advanced biosensing and bioanalysis techniques
Ruhr University Bochum
2020-2024
Neurotransmitters are important signaling molecules in the brain and relevant many diseases. Measuring them with high spatial temporal resolutions biological systems is challenging. Here, we develop a ratiometric fluorescent sensor/probe for catecholamine neurotransmitters on basis of near-infrared (NIR) semiconducting single wall carbon nanotubes (SWCNTs). Phenylboronic acid (PBA)-based quantum defects incorporated into to interact selectively catechol moieties. These PBA-SWCNTs further...
We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT are bright in unbound state diminish their fluorescence upon binding 5-HT. Sensor variants with different affinities for were engineered to increase versatility imaging dynamics. Experiments vitro vivo showed feasibility dynamics high temporal spatial resolution. As demonstrated here, designed show excellent membrane expression, have...
Neural glutamate homeostasis plays a key role in health and disease. In ischemic conditions, such as stroke, this is severely disrupted since energy depletion ion imbalances lead to more release less uptake. We here used the fluorescent sensor SF iGluSnFR(A184V) probe effects of chemical ischemia on extracellular dynamics situ, using organotypic slice cultures from mouse cortex. iGluSnFR imaging reported spontaneous events, which indicate synchronous network activity, similar calcium signals...
NMDA receptors are important players for neuronal differentiation. We previously reported that antagonizing with APV blocked the growth-promoting effects evoked by overexpression of specific calcium-permeable or flip-spliced AMPA receptor subunits and type I transmembrane regulatory proteins which both exclusively modify apical dendritic length branching cortical pyramidal neurons. These findings led us to characterize role GluN2B GluN2A dendritogenesis using organotypic cultures rat visual...
Abstract Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio red-shifted posed significant challenges. More critically, several red-fluorescent exhibit photoswitching when exposed to blue light, thereby limiting applicability in all-optical experimental approaches. Here, we present the...
Neurotransmitters are important signaling molecules in the brain and relevant many diseases. Measuring them with high spatial temporal resolution biological systems is challenging. Here, we develop a ratiometric fluorescent sensor/probe for catecholamine neurotransmitters based on near-infrared (NIR) semiconducting single wall carbon nanotubes (SWCNTs). Phenylboronic acid (PBA)-based quantum defects incorporated into to interact selectively catechol moieties. These PBA-SWCNTs further...
Abstract We developed a new family of genetically encoded serotonin (5-HT) sensors ( sDarken ) on the basis native 5-HT 1A receptor and circularly permuted GFP. are bright in unbound state diminish their fluorescence upon binding 5-HT. Sensor variants with different affinities for were engineered to increase versatility imaging dynamics. Experiments vitro vivo showed feasibility dynamics high temporal spatial resolution. As demonstrated here, designed excellent membrane expression, have...