A5024831770- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Sleep and Wakefulness Research
- Optical Imaging and Spectroscopy Techniques
- Animal Behavior and Welfare Studies
- Reinforcement Learning in Robotics
- Neuroscience and Neural Engineering
- Zebrafish Biomedical Research Applications
- Animal Behavior and Reproduction
- Olfactory and Sensory Function Studies
- Gene Regulatory Network Analysis
University of Zurich
2021-2024
ETH Zurich
2020-2024
Neurosciences Institute
2023-2024
Neuroscience Institute
2023-2024
Department of Medical Sciences
2023-2024
Zürcher Fachhochschule
2022
Abstract An essential feature of neurons is their ability to centrally integrate information from dendrites. The activity astrocytes, in contrast, has been described as mostly uncoordinated across cellular compartments without clear central integration. Here we report conditional integration calcium signals astrocytic distal processes at soma. In the hippocampus adult mice both sexes, found that global activity, recorded with population imaging, reflected past neuronal and behavioral events...
Abstract The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, the underlying molecular cascades are unclear. Here, we use a brief swim exposure trigger an in mice, which transiently increases anxiety, without leading lasting maladaptive changes. Using multiomic profiling, such as proteomics, phospho-proteomics, bulk mRNA-, single-nuclei small RNA-, TRAP-sequencing, characterize stress-induced events mouse hippocampus over time. Our...
Abstract Noradrenaline (NA) release from the locus coeruleus (LC) changes activity and connectivity in neuronal networks across brain, modulating multiple behavioral states. NA is mediated by both tonic burst-like LC activity. However, it unknown whether functional target areas depend on these firing patterns. Using optogenetics, photometry, electrophysiology magnetic resonance imaging mice, we show that patterns elicit brain responses hinge their distinct dynamics. During moderate...
Abstract Noradrenaline (NA) release from the brainstem nucleus locus coeruleus (LC) changes activity and connectivity in neuronal networks across brain, thus modulating multiple behavioural states. NA is mediated by both tonic burst-like LC activity. However, it remains unknown whether functional downstream projection areas depend on these firing patterns. Using optogenetics, pupillometry, photometry, MRI mice, we show that burst patterns elicit brain responses are specific to activation...
Exposure to an acute stressor triggers a complex cascade of neurochemical events in the brain. However, deciphering their individual impact on stress-induced molecular changes remains major challenge. Here, we combine RNA sequencing with selective pharmacological, chemogenetic, and optogenetic manipulations isolate contribution locus coeruleus-noradrenaline (LC-NA) system stress response mice. We reveal that NA release during exposure regulates large reproducible set genes dorsal ventral...
Abstract An essential feature of neurons is their ability to centrally integrate information from dendrites. The activity astrocytes, in contrast, has been described as mostly uncoordinated across cellular compartments without clear central integration. Here, we describe conditional centripetal integration a principle how astrocytes calcium signals distal processes induce somatic activation. We found mouse hippocampus that global astrocytic activity, recorded with population imaging, well...
Information seeking, such as standing on tiptoes to look around in humans, is observed across animals and helps survival. Its rodent analog—unsupported rearing hind legs—was a classic model deciphering neural signals of cognition intense renewed interest preclinical modeling neuropsychiatric states. Neural circuits controlling this dedicated decision seek information remain largely unknown. While studying subsecond timing spontaneous behavioral acts activity melanin-concentrating hormone...
Abstract Exposure to an acute stressor triggers a complex cascade of neurochemical events in the brain. However, deciphering their individual impact on stress-induced molecular changes remains major challenge. Here we combine RNA-sequencing with selective pharmacological, chemogenetic and optogenetic manipulations isolate contribution locus coeruleus - noradrenaline (LC-NA) system stress response. We reveal that NA-release during exposure regulates large reproducible set genes dorsal ventral...
Exposure to an acute stressor triggers a complex cascade of neurochemical events in the brain. However, deciphering their individual impact on stress-induced molecular changes remains major challenge. Here, we combine RNA sequencing with selective pharmacological, chemogenetic, and optogenetic manipulations isolate contribution locus coeruleus-noradrenaline (LC-NA) system stress response mice. We reveal that NA release during exposure regulates large reproducible set genes dorsal ventral...
Chronic stress exposure in adolescence can lead to a lasting change responsiveness later life and is associated with increased mental health issues adulthood. Here we investigate whether the Social Instability (CSI) paradigm influences behavioral molecular responses novel acute stressors mice, it alters physiological influenced by noradrenergic system. Using large cohorts of show that CSI mice display persistent increase exploratory behaviors open field test alongside small but widespread...
Abstract The nuanced detection of rodent behavior in preclinical biomedical research is essential for understanding disease conditions, genetic phenotypes, and internal states. Recent advances machine vision artificial intelligence have popularized data-driven methods that segment complex animal into clusters behavioral motifs. However, despite the rapid progress, several challenges remain: Statistical power typically decreases due to multiple testing correction, poor transferability...
ABSTRACT Exploration is essential for survival because it allows animals to gather information about their environment. Rearing a classic exploratory behavior, during which an animal transiently stands on its hind legs sample It widely observed in common lab conditions as well the wild, yet neural signals and circuits underlying this fundamental component of innate behavior remain unclear. We examined behavioral correlates activity hypothalamic MCH-producing neurons (MNs) – recently...
Summary The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, little is known about the nature dynamics of underlying molecular cascades. We used a brief forced swim exposure trigger strong in mice, which transiently increases anxiety, but does not lead lasting maladaptive behavioral changes. Using multiomic profiling we characterize stress-induced events hippocampus over time, across scales, down level cell types single cells. Our...
Exposure to an acute stressor triggers a complex cascade of neurochemical events in the brain. However, deciphering their individual impact on stress-induced molecular changes remains major challenge. Here we combine RNA-sequencing with selective pharmacological, chemogenetic and optogenetic manipulations isolate contribution locus coeruleus - noradrenaline (LC-NA) system stress response. We reveal that NA-release during exposure regulates large reproducible set genes dorsal ventral...
The accurate detection and quantification of rodent behavior forms a cornerstone basic biomedical research. Current data-driven approaches, which segment free exploratory into clusters, suffer from low statistical power due to multiple testing, exhibit poor transferability across experiments fail exploit the rich behavioral profiles individual animals. Here we introduce pipeline capture each animal's flow, yielding single metric based on all observed transitions between clusters. By...
Exposure to an acute stressor triggers a complex cascade of neurochemical events in the brain. However, deciphering their individual impact on stress-induced molecular changes remains major challenge. Here we combine RNA-sequencing with selective pharmacological, chemogenetic and optogenetic manipulations isolate contribution locus coeruleus - noradrenaline (LN-NA) system stress response. We reveal that NA-release during exposure regulates large reproducible set genes dorsal ventral...
<title>Abstract</title> Preclinical biomedical research depends on accurately detecting and quantifying rodent behavior. Current data-driven approaches, which segment free exploratory behavior into clusters, suffer from low statistical power due to multiple-testing, exhibit poor transferability across experiments, fail exploit the rich behavioral profiles of individual animals. We introduce a pipeline capture each animal’s flow, yielding single metric based all observed transitions between...