A5015555425- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Evolutionary Algorithms and Applications
- Cardiac electrophysiology and arrhythmias
- Psychosomatic Disorders and Their Treatments
- Vagus Nerve Stimulation Research
- EEG and Brain-Computer Interfaces
- Luminescence Properties of Advanced Materials
- Gaze Tracking and Assistive Technology
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Fish biology, ecology, and behavior
- Atmospheric chemistry and aerosols
- Acupuncture Treatment Research Studies
- Per- and polyfluoroalkyl substances research
- Ion channel regulation and function
- Analytical Chemistry and Sensors
- Optical Coherence Tomography Applications
- Luminescence and Fluorescent Materials
- Neuroscience and Neural Engineering
- Toxic Organic Pollutants Impact
Chinese Academy of Sciences
2011-2024
Jinan University
2023-2024
Shenzhen Institute of Neuroscience
2020-2024
Center for Excellence in Brain Science and Intelligence Technology
2015-2024
University of Chinese Academy of Sciences
2020
Shenzhen University
2020
Shanghai Center for Brain Science and Brain-Inspired Technology
2020
Shanghai Institutes for Biological Sciences
2004-2017
New York Institute of Technology
2004
The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging 3D tracking technique monitors whole neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional visually evoked prey capture behaviors zebrafish.
A highly sensitive and selective near-infrared excited potassium nanosensor has been developed for brain activity monitoring.
The hyperpolarization-activated cation channels ( I h ) play a distinct role in rhythmic activities variety of tissues, including neurons and cardiac cells. In the present study, we investigated whether Ca 2+ can permeate through pacemaker (HCN) expressed HEK293 cells dorsal root ganglion (DRG) neurons. Using combined measurements whole-cell currents fura-2 imaging, found that there is influx proportion to induced by hyperpolarization channel blockers Cs + ZD7288 inhibit both HCN current...
Optical voltage sensors with the ability to monitor neuronal activities are invaluable tools for studying information processing of brain. However, current genetically encoded indicators usually require high-power visible light excitation and limited addressable model animals. Here, we report a near-infrared (NIR)-excited nongenetic nanosensor that achieves stable recording membrane potential in intact The is composed Förster resonance energy transfer (FRET) pair, outer membrane-anchored...
Abstract Visualization of axons and dendritic spines is crucial in neuroscience research. However, traditional microscopy limited by diffraction-limited resolution shallow imaging depth, making it difficult to study neuronal dynamics. Two-photon multifocal structured illumination (2P-MSIM) provides super-resolution along with a reasonably good penetration, but vulnerable optical aberrations deep tissues. Herein we present novel non-inertial scanning 2P-MSIM system incorporated adaptive...
All-optical interrogation of population neuron activity is a promising approach to deciphering the neural circuit mechanisms supporting brain functions.However, this currently limited local areas.Here, we incorporate patterned photostimulation into light-sheet microscopy, allowing simultaneous targeted optogenetic manipulation and brain-wide monitoring neuronal activities head-restrained behaving larval zebrafish.Using system, photo-stimulate arbitrarily selected neurons (regions as small...
Periodic sensory stimuli are prevalent in natural environment and may signal events of particular importance. However, whether periodic aperiodic differentially processed by neural circuits remains unclear. Here we show that exert influences over longer distances than at both neuronal perceptual levels. Whole-cell recording from rat visual cortex showed stimulation (1-11 Hz) outside the receptive field evoked robust membrane potential oscillation frequency, while same stimulus applied...
Dopamine plays crucial roles in a broad spectrum of brain functions, and neural circuit mechanisms underlying dopaminergic regulation have been intensively studied the past decade. As larval zebrafish relatively simple highly conserved systems, it can serve as an ideal vertebrate animal model to tackle this issue at whole-brain scale. For purpose, is important develop methods for monitoring endogenous dopamine release intact zebrafish. Here, we developed real-time method monitor high...
Zebrafish is a relatively new vertebrate animal model with conserved brain architecture and rich repertoire of behaviors. In recent years, we have witnessed the development multiple approaches tailored for it, including in vivo electrophysiology, optical imaging genetic manipulations. Due to transparency simplicity brain, larval zebrafish has emerged as an ideal dissecting functions at whole-brain scale based on strategy from synapses, neurons, circuitries this review, will summarize...
Abstract The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here we report on a novel volume imaging 3D tracking technique monitors whole neural activity in freely swimming larval zebrafish ( Danio rerio ). We demonstrated the capability of our system through functional visually evoked prey capture behaviors zebrafish.
We developed a new type of light field microscope with extended field-of-view. Using this technique, we achieved whole larval zebrafish brain imaging at near single cell resolution and 90 Hz volume rate. demonstrated its applications in functional neural dynamics fast circulating blood cells brain.
In neuroscience, it's one of the central goals to decipher neuronal ensembles' function in neural circuits. Recently, progress probes for optogenetic actuators and calcium indicators afford probability simultaneous optical manipulation imaging ensembles. Although with point-scanning localized stimulation, previous work had achieved all-optical interrogation circuits local brain areas mice zebrafish larvae, no attempt was made interrogate across whole-brain. Here, we combined fast volumetric...