A5100556441- Insect Resistance and Genetics
- Pesticide and Herbicide Environmental Studies
- Insect-Plant Interactions and Control
- Pharmaceutical and Antibiotic Environmental Impacts
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Soil Carbon and Nitrogen Dynamics
- Insect Pest Control Strategies
- Fungal Biology and Applications
- Plant Stress Responses and Tolerance
- Molecular Biology Techniques and Applications
- Mycorrhizal Fungi and Plant Interactions
- Enzyme-mediated dye degradation
- Viral Infectious Diseases and Gene Expression in Insects
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
- Weed Control and Herbicide Applications
- Allelopathy and phytotoxic interactions
- Forest Ecology and Biodiversity Studies
- Composting and Vermicomposting Techniques
- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- RNA Research and Splicing
Heilongjiang University
2015-2025
Shaanxi Polytechnic Institute
2022
Education Department of Heilongjiang Province
2020-2021
Institute of Vegetables and Flowers
2016
Chinese Academy of Agricultural Sciences
2016
Hebei Agricultural University
2008
Significance Most studies of pesticide resistance have focused on the identification and functional analysis genes, but few considered signaling pathways involved in their regulation. In this work, we discovered that overexpression a P450 confers to neonicotinoid insecticides whitefly is trans -regulated by transcription factor CREB . Further demonstrated exposure activates key pathway cellular response extracellular signals, MAPK pathway, phosphorylation. then binds specific site promoter...
The soil insect Bradysia odoriphaga (Diptera: Sciaridae) causes substantial damage to Chinese chive. Suitable reference genes in B. (Bradysia odoriphaga) have yet be identified for normalizing target gene expression among samples by quantitative real-time PCR (qRT-PCR). This study was focused on identifying the stability of 12 candidate housekeeping under various experiment conditions. final ranking obtained with RefFinder, and most suitable number analyzed GeNorm. results revealed that...
Applying decomposition agents for straw return can not only accelerate decomposing, but also reduce the use of chemical fertilizers and improve soil fertility. However, mechanism applying agent on environment is clear yet. This study investigated effects returning with a during four stages wheat growth development. The application decomposers increased Total Nitrogen (TN) content in by 237.7% 102.2% at heading maturity stages, respectively. Available Phosphorus (AP) jointing stage 10.3%....
The efficient degradation of residues prometryn is essential as it widely used to control weeds in wheat fields, but its the soil may lead a decrease fertility and even affect growth subsequent crops. In this experiment, we isolated T6-2 T7-2 strains, analyzed morphological, physiological biochemical characteristics 16S rRNA homology T7-2, investigated effects different pH, inoculum incubation time on rate strains. were identify strain Bacillus subtilis subsp. Subtilis Brevundimonas...
Abstract Corn defense systems against insect herbivory involve activation of genes that lead to metabolic reconfigurations produce toxic compounds, proteinase inhibitors, oxidative enzymes and behavior-modifying volatiles. Similar responses occur when the plant is exposed methyl jasmonate (MeJA). To compare between stalk borer feeding exogenous MeJA on a transcriptional level, we employed deep transcriptome sequencing methods following Ostrinia furnacalis leaf treatment. 39,636 were found be...
Abstract Arbuscular mycorrhizal fungi (AMF) protect host plants against diverse biotic and abiotic stresses promote biodegradation of various contaminants. In this study effect Glomus mosseae/Medicago sativa mycorrhiza on atrazine degradation was investigated. It observed that the rates with any addition level in treatments were all significantly higher than those non- treatments. When applied at 20 mg kg −1 , removal efficiency up to 74.65%. Therefore, G. mosseae can be considered as ideal...
Rhopalosiphum padi (L.) (Hemiptera: Aphididae) is an important cosmopolitan pest in cereal crops. Reference genes can significantly affect qRT-PCR results. Therefore, selecting appropriate reference a key prerequisite for analyses. This study was conducted to identify suitable R. . We systematically analyzed the expression profiles of 11 commonly used genes. The ΔCt method, BestKeeper, NormFinder, geNorm algorithms, and RefFinder online tool were evaluate suitability these under diverse...
Abamectin has been used to control the diamondback moth, Plutella xylostella (P. xylostella), which is a major agricultural pest that can rapidly develop resistance against insecticides including abamectin. Although cytochrome P450 confirmed play an important role in P. xylostella, specific genes associated with are unclear. The full-length cDNA of gene CYP340W1 was cloned and characterized present study. assembly yielded sequence 1929 bp, containing open reading frame (ORF) 1491 bp encodes...
Abstract Background Due to being rooted in the ground, maize ( Zea mays L.) is unable actively escape attacks of herbivorous insects such as Asian corn borer Ostrinia furnacalis ). In contrast passive damage, plants have evolved defense mechanisms protect themselves from herbivores. Salicylic acid, a widely present endogenous hormone plants, has been found play an important role inducing plant resistance insects. this study, we screened and identified insect gene SPI , which simultaneously...
Sugar transporters play pivotal roles in the growth, development, and stress responses of phloem-sucking insects, such as whitefly, Bemisia tabaci. In this study, 137 sugar (STs) were identified based on analysis genome transcriptome B. tabaci MEAM1. MEAM1 encodes a larger number STs than other selected insects. Phylogenetic molecular evolution showed that formed three expanded clades genes Sternorrhyncha had accelerated rates evolution. (BTSTs) divided into groups their expression profiles...
The whitefly (Bemisia tabaci), an important invasive pest that causes severe damage to crops worldwide, has developed resistance a variety of insecticides. Carboxylesterases (COEs) are multifunctional enzymes involved in the growth, development, and xenobiotic metabolism insects. However, systematic studies on COEs B. tabaci scarce. Here, 42 putative different functional categories were identified Mediterranean species (B. MED) based genome database neighbor-joining phylogeny. expression...
An allele of the cytochrome P450 gene, CYP6AE14, named CYP6AE25 (GenBank accession no. EU807990) was isolated from Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae) by RT-PCR. The cDNA sequence is 2315 bp in length and contains a 1569 nucleotides open reading frame encoding putative protein with 523 amino acid residues predicted molecular weight 59.95 kDa theoretical pI 8.31. classic heme-binding motif F××G×××C×G (residues 451-460) conserved among all enzymes as well...
Atrazine has been extensively used in China’s agricultural production for a long time and the potential risks to environment have received widespread attention. The purpose of this study was examine effect continuous application atrazine on soil herbicide residues, enzyme activity, microbial community structure, as well provide theoretical reference appropriate improvement soil. Previous studies focused effects microorganisms, but experiments higher doses than recommended. To reveal actual...
Bradysia odoriphaga (Diptera: Sciaridae) is the most serious root maggot pest which causes substantial damage to Chinese chive. Organophosphate (OP) and neonicotinoid insecticides are widely used chemical pesticides play important roles in controlling B. . However, a strong selection pressure following repeated pesticide applications has led development of resistant populations this insect. To understand insecticide resistance mechanism , gene expression analysis might be required....
Glutathione-S-transferases (GST) comprise a multifunctional protein superfamily, which plays important roles as detoxifiers and antioxidants in insects. The GST Asian corn borer has not been previously characterized. In this study, we cloned, characterized, expressed the complete genes from midgut of borer. Furthermore, designed htL4440-OfGST vector to exploit gene for RNA interference (RNAi) strategy control pest. A cDNA sequence was obtained by reverse transcription polymerase chain...
The whitefly (Bemisia tabaci) is a cosmopolitan and devastating pest of agricultural crops ornamentals.B. tabaci causes extensive damage by feeding on phloem transmitting plant viruses.Like many other organisms, insects depend amino acid transporters (AATs) to transport acids into out its cells.We present genome-wide transcriptome-wide investigation the following two families AATs in B. biotype B: acid/auxin permease (AAAP) acid/polyamine/organocation (APC).A total 14 putative APCs 25 AAAPs...
Herbicidal residues of imazamox are hazardous to some sensitive rotational aftercrops. The aim the study was isolate and identify a microbial strain capable degrading imazamox. IB5, efficiently imazamox, isolated from an imazamox-contaminated soybean field in Heilongjiang Province, China. It found degrade 98.61% 400 mg L−1 within 48 h by high-performance liquid chromatograph. Through morphological, physiological biochemical characterization, 16S rDNA sequencing, identified as Acinetobacter...
Atrazine is a long residual herbicide commonly used in maize fields. Although atrazine can effectively control weeds and improve crop yield, long-term application leads to continuous pollution the agricultural ecological environment, especially soil ecosystem, its impact on microorganisms still not clear. Four methods were experiment clarify effect of bacterial populations cultivated layers chernozem cold region different periods: high-performance liquid chromatography (HPLC), colorimetry,...
Soil salinization is currently one of the main abiotic stresses that restrict plant growth. Plant endophytic bacteria can alleviate stress. The aim current study was to isolate, characterize, and assess growth-promoting saline alkaline stress-alleviating traits
Based on fomesafen residue toxicity in soil, the strain FB5, which can utilize as sole carbon source, was isolated from a fomesafen-contaminated soybean field Heilongjiang province, China. It found to degrade 88.32% of 500 mg/L within 96 h. Conbined with morphological, physiological, biochemical characteristics and 16S rDNA sequence, identified Shigella flexneri. After evaluating effects environmental factors such temperature, concentration, primary pH, optimum growth conditions were...