A5110038237- Neuroscience of respiration and sleep
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Prion Diseases and Protein Misfolding
- Nitric Oxide and Endothelin Effects
- Neurological diseases and metabolism
- Trace Elements in Health
- Neonatal and fetal brain pathology
- Neonatal Health and Biochemistry
- Porphyrin and Phthalocyanine Chemistry
- Congenital Diaphragmatic Hernia Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- bioluminescence and chemiluminescence research
- Adenosine and Purinergic Signaling
- Neuroendocrine regulation and behavior
- Drug Transport and Resistance Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Vagus Nerve Stimulation Research
- Garlic and Onion Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
University of Pittsburgh
2024
Case Western Reserve University
2000-2014
Western University of Health Sciences
2012
Rainbow Babies & Children's Hospital
2006-2011
Mayo Clinic
2011
Howard University
2004-2006
University Hospitals of Cleveland
1992-1993
Abstract Very little is known about the applicability of metabolic and biochemical events observed in cell culture systems to vivo tumor shrinkage following photodynamic therapy (PDT). The purpose this study was assess whether PDT induces apoptosis during ablation . We treated radiation‐induced fibrosarcoma (RIF‐1) tumors grown C3H/HeN mice with employing three photosensitizers, Photofrin‐II, chloroaluminum phthalocyanine tetrasulfonate, or Pc IV (a promising developed laboratory). Each...
The conversion of soluble prion protein into an insoluble, pathogenic, protease-resistant isoform is a key event in the development diseases. Although mechanism by which engenders pathogenic unclear, there increasing evidence to suggest that this may depend on function preventing oxidative damage. Therefore, study, we assessed interrelationship between redox-sensitive cysteine, glycosylation, and metabolism. Cells were treated with thioreductant, dithiothreitol, assess effect cellular...
Abstract Background and Aim: Our previous study of Helicobacter pylori ‐induced apoptosis showed the involvement Bcl‐2 family proteins cytochrome c release from mitochondria. Here, we examine other factors mitochondria, such as apoptosis‐inducing factor (AIF), upstream events involving caspase‐8 Bid. Methods: Human gastric adenocarcinoma (AGS) cells were incubated with a cagA‐positive H. strain for 0, 3, 6, 24 hours either total protein or cytoplasmic, nuclear, mitochondrial membrane...
<i>Background:</i> Prolonged exposure of immature lungs to hyperoxia contributes neonatal lung injury and airway hyperreactivity. We have previously demonstrated that rat pups ≧95% O<sub>2</sub> impairs relaxation due disruption nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) signaling. <i>Objective:</i> now hypothesize these impaired responses are secondary hyperoxia-induced upregulation arginase, which competes with NO synthase for...
The abnormal form of the prion protein has increased resistance to protease digestion and is insoluble in non-ionic detergents. normal modified by non-obligatory addition two N-linked glycans. One pathogenic mutation, Thr Ala at residue 183 human protein, blocks first glycan Asp 181. This mutation been reported result intracellular retention mutant its acquisition properties, presumably due lack glycan. We report that 181 not responsible for block transport or pathogen-like rather, itself...
Abstract— Photodynamic therapy (PDT) of cancer is a modality that relies upon the irradiation tumors with visible light following selective uptake photosensitizer by tumor tissue. There considerable emphasis to define new photosensitizers suitable for PDT cancer. In this study we evaluated six phthalocyanines (Pc) their photodynamic effects utilizing rat hepatic microsomes and human erythrocyte ghosts as model membrane sources. Of newly synthesized Pc, two showed significant destruction...
Airway hyperreactivity is one of the hallmarks hyperoxic lung injury in early life. As neurotrophins such as brain-derived neurotrophic factor (BDNF) and nerve growth (NGF) are potent mediators neuronal plasticity, we hypothesized that neurotrophin levels pulmonary system may be disturbed by exposure. We therefore evaluated effects hyperoxia on expression BDNF, NGF, their corresponding high-affinity receptors, TrkB TrkA, respectively, rat pups. Five-day-old Sprague-Dawley pups were...
Neurally derived tachykinins such as substance P (SP) play a key role in modulating airway contractility (especially with inflammation). Separately, the neurotrophin brain-derived neurotrophic factor (BDNF; potentially from nerves well smooth muscle; ASM) and its tropomyosin-related kinase receptor, TrkB, are involved enhanced contractility. In this study, we hypothesized that neurokinins neurotrophins linked enhancing intracellular Ca(2+) concentration ([Ca(2+)](i)) regulation ASM. rat ASM...
Exposure of immature lungs to hyperoxia for prolonged periods contributes neonatal lung injury and airway hyperreactivity. We studied the role disrupted nitric oxide-guanosine 3',5'-cyclic monophosphate (NO-cGMP) signaling in impairing relaxant responses tissue from hyperoxia-exposed rat pups. Pups were exposed >/=95% O(2) or room air 7 days starting 1, 5, 14. The animals killed, removed, 1-mm-thick parenchymal strips prepared. Lung hyperoxic pups preconstricted using bethanechol then graded...
Prolonged hyperoxic exposure contributes to neonatal lung injury, and airway hyperreactivity is characterized by enhanced contraction impaired relaxation of smooth muscle. Our previous data demonstrate that hyperoxia in rat pups upregulates expression brain-derived neurotrophic factor (BDNF) mRNA protein, disrupts NO-cGMP signaling, impairs cAMP production We hypothesized BDNF-tyrosine kinase B (TrkB) signaling plays a functional role via upregulation cholinergic mechanisms hyperoxia-exposed...
Prion diseases are associated with the accumulation of a misfolded, protease resistant form prion protein, PrPres. In humans there variety different related that sporadic, inherited, or acquired by infection. Gerstmann-Straussler-Sheinker syndrome (GSS) is an inherited disease in which PrPres accumulates as amorphous aggregates well amyloid plaques. GSS has been point mutations protein: 102, 105, 117, 131, 145, 187, 198, 202, 212, 217, and 232. The F198S mutation was discovered large Indiana...
Mechanisms of cell death have been explored in cells and tumors treated with photodynamic therapy (PDT). Photosensitizers used for these studies were Photofrin, tetrasulfonated nonsulfonated aluminum phthalocyanine, a new silicon phthalocyanine [SiPc(OH)OSi(CH<SUB>3</SUB>)<SUB>2</SUB>(CH<SUB>2</SUB>)<SUB>3</SUB>N(CH<SUB>3</SUB>)<SUB>2</SUB>], referred to as PcIV. In mouse lymphoma L5178Y cells, dose PDT sensitized by PcIV which causes 90% loss survival induces apoptosis (programmed death)...
Abstract Photodynamic therapy (PDT) of cancer is an experimental tumor which based on the combined use a systemically administered photo‐sensitizer to tumor‐bearing host and local illumination lesion by high‐intensity visible light source, typically tunable argon dye laser. Human squamous cell carcinoma (HSCC) most frequently encountered malignancy head neck. In this study, responses HSCC cells PDT were examined in vitro vivo systems. studies, showed positive photodynamic response with...