A5090935103- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Glutathione Transferases and Polymorphisms
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Biochemical and Molecular Research
- Genomics, phytochemicals, and oxidative stress
- Insect behavior and control techniques
- Ovarian function and disorders
- Medical Imaging and Analysis
- Abdominal vascular conditions and treatments
- Nutrition, Genetics, and Disease
- RNA and protein synthesis mechanisms
- Vasculitis and related conditions
- Reproductive tract infections research
- Cancer, Hypoxia, and Metabolism
- Reproductive Health and Technologies
- Chromosomal and Genetic Variations
- Renal and Vascular Pathologies
- Epigenetics and DNA Methylation
- Cytomegalovirus and herpesvirus research
- Diabetic Foot Ulcer Assessment and Management
- Reproductive Health and Contraception
Peking University
2023-2024
Center for Life Sciences
2023-2024
Capital Medical University
2024
China-Japan Friendship Hospital
2024
Imperial College London
2023-2024
Zhongda Hospital Southeast University
2023
Anhui Medical University
2014
Pamela Youde Nethersole Eastern Hospital
2002
Karolinska Institutet
1994-2002
The DNA repair protein xeroderma pigmentosum complementation group D (XPD) is involved in the nucleotide excision of lesions induced by many tobacco and environmental carcinogens. In order to study functional impact common polymorphisms XPD exon 10 (G > A, Asp312Asn) 23 (A C, Lys751Gln), we have genotyped 185 Swedish lung cancer cases (97 smokers 88 never-smokers) 162 matched population controls (83 79 never-smokers). Presence one or two variant alleles was associated with increased risk for...
Homing-based gene drives are recently proposed interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set in tephritid agricultural pest species, Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that medfly is highly amenable to homing-based drive strategies. By targeting transformer gene, also demonstrate how CRISPR-Cas9 can be coupled sex conversion, whereby females transformed into fertile...
Gene drive technology has the potential to address major biological challenges. Well-studied homing suppression drives have been shown be highly efficient in Anopheles mosquitoes, but for other organisms, lower rates of conversion prevent elimination target population. To tackle this issue, we propose a gene design that two targets: site where takes place, and distant cleavage induces population suppression. We model find two-target system allows occur over much wider range efficiency....
We have genotyped 657 Norwegian men, including 282 lung cancer patients (147 non-operable and 135 operable) 375 healthy referents (210 smokers 165 non-smokers), to study the possibility that glutathione S-transferase M1 (GSTM1)-null and/or N-acetyl transferase 2 (NAT2)-slow genotypes confer susceptibility towards in smokers. Compared with smoking referents, there was a significant over-representation of GSTM1-null genotype among squamous cell carcinoma (SQ) [odds ratio (OR) = 1.7, 95%...
This study aims to understand the association between body roundness index (BRI) and female infertility prevalence. Infertility is a public health concern with significant implications for individuals' well-being rights.
The influence of the metabolic genotypes GSTM1 and NAT2 on urinary excretion mutagens in 46 coke oven workers (27 them smokers) was studied. Exposure to polycyclic aromatic hydrocarbons (PAH) estimated from 1-pyrenol levels, which varied 0.23 5.59 micromol/mol creatinine. Fourteen urine samples (30.4%), all but one belonging smokers, were positive for mutagenic activity (i.e. at least assayed doses able double number spontaneous revertants). Nine urine-positive subjects both GSTM1-null...
The hypoxanthine-guanine phosphoribosyl transferase (hprt) locus in 6-thioguanine (TG) resistant T-lymphocytes is a useful target for the study of somatic vivo mutagenesis, since it provides information about broad spectrum mutation. Mutations hprt coding region were studied 124 TG-resistant T-cell clones from 38 healthy, non-smoking male donors previously population bus maintenance workers, fine-mechanics and laboratory personnel. Their mean age was 43 years (range 23-64) their mutant...
Abstract Homing-based gene drives are novel interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set in tephritid agricultural pest species, Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that medfly is highly amenable to homing-based drive strategies. By targeting transformer gene, also demonstrate two different mechanisms by which CRISPR-Cas9 can be coupled sex conversion, whereby females...
Abstract Gene drive technology has the potential to address major biological challenges, including management of disease vectors, invasive species, and agricultural pests. After releasing individuals carrying gene in target population, suppression drives are designed spread at a rapid rate carry recessive fitness cost, thus bringing about decline population size or even complete suppression. Well-studied homing have been shown be highly efficient Anopheles mosquitoes were successful...
Provisional mutational spectra at the hypoxanthine phosphoribosyl transferase (HPRT) locus in vitro have been worked out for acetaldehyde (AA) and benzo[a]pyrene diolepoxide (BPDE) human (T)-lymphocytes ethylene oxide (EtO) diploid fibroblasts using Southern blotting polymerase chain reaction (PCR)-based DNA sequencing techniques. The results indicate that large genomic deletions are predominating hprt mutations caused by AA EO, whereas BPDE induces point mainly GC > TA transversions....
Gene drives are engineered alleles that bias their own inheritance in offspring, enabling the spread of specific traits throughout a population. Targeting female fertility genes gene drive can be an efficient strategy for population suppression. In this study, we investigated nine Drosophila melanogaster using CRISPR-based homing drives. Employing multiplexed gRNA approach to prevent formation functional resistance alleles, aimed maintain high conversion efficiency with low fitness costs...
Objective: To investigate the consistency of skeletal muscle mass by CT at 1st lumbar vertebrae (L1) and 3rd (L3) levels correlation density (SMD) L1 level with prognosis in dialysis patients. Methods: A total 1 020 patients who underwent initial had examination data four centers (Zhongda Hospital Affiliated to Southeast University, Third Soochow Taizhou People's Nanjing Medical University Yangzhou University) from January 2014 December 2019 were retrospectively collected. The index (SMI)...
Abstract CRISPR homing gene drive is a potent technology with considerable potential for managing populations of medically and agriculturally significant insects. It induces bias in the inheritance allele progeny, rapidly spreading desired genes throughout population. Homing drives operate by Cas9 cleavage followed homology-directed repair, copying to wild-type chromosome. However, resistance alleles formed end-joining repair pose obstacle spread drive. To address this challenge, we created...
Introduction: Renal artery pseudoaneurysms may arise as a complication of percutaneous nephrolithotomy (PCNL). Prompt recognition and treatment is essential to arrest haemorrhage which be life threatening. Clinical Picture: A patient with chronic aortic dissection malrotated right kidney underwent PCNL for renal calculus. He developed delayed gross haematuria. Treatment: Angiography showed pseudoaneurysm arising from one two arteries, in turn arose the false lumen dissection. The supplying...