A5108110774- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Characterization and Applications of Magnetic Nanoparticles
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Anesthesia and Neurotoxicity Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Microbial Inactivation Methods
- Digital Imaging for Blood Diseases
- Bladder and Urothelial Cancer Treatments
- Air Quality and Health Impacts
- Electrical and Bioimpedance Tomography
- Protein Interaction Studies and Fluorescence Analysis
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Neuroscience and Neural Engineering
- Orthopaedic implants and arthroplasty
- Health, Environment, Cognitive Aging
- Redox biology and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- Medical Imaging Techniques and Applications
- Cancer-related gene regulation
Ljubljana University Medical Centre
2025
University of Ljubljana
2014-2022
Highlights:•Most laboratory parameters returned to reference values 6 months after COVID-19 infection•Values of certain still correlated with severity•Values suggest incomplete resolution renal, biliary tract, and immune system functionAbstractObjectivesDespite extensive study disease, only a few studies also addressed the aftermath disease potential long-term consequences. The aim this was assess through cross-sectional analysis an range haematological biochemical find markers associated...
Abstract: Magnetic nanoparticles (NPs) are a special type of NP with ferromagnetic, electron-dense core that enables several applications such as cell tracking, hyperthermia, and magnetic separation, well multimodality. So far, superparamagnetic iron oxide NPs (SPIONs) the only clinically approved metal NPs, but cobalt ferrite have properties suitable for biomedical well. In this study, we analyzed cellular responses to coated polyacrylic acid (PAA) in three types: Chinese Hamster Ovary...
Alongside physiochemical properties (PCP), it has been suggested that the protein corona of nanoparticles (NPs) plays a crucial role in response immune cells to NPs. However, due great variety NPs, target cells, and exposure protocols, there is still no clear relationship between PCP, composition, immunotoxicity In this study, we correlated PCP composition NPs THP-1 macrophage response, focusing on selected toxicological endpoints: cell viability, reactive oxygen species (ROS), cytokine...
Introduction of genetic material into muscle tissue has been extensively researched, including isolation and in vitro expansion primary myoblasts as a potential source cells for skeletal heart engineering applications. In this study, we optimized the electroporation protocol introduction short interfering ribonucleic acid (siRNA) against messenger RNA Hypoxia Inducible Factor 1α (HIF‐1α) cultured human myoblasts. We established optimal pulsing siRNA electro transfection, theoretically...
Summary Cell counting in microscopic images is one of the fundamental analysis tools life sciences, but usually tedious, time consuming and prone to human error. Several programs for automatic cell have been developed so far, most them demand additional training or data input from user. Most do not allow users online monitor results, either. Therefore, we designed two straightforward, simple‐to‐use cell‐counting that also correct detection results. In this paper, present Cellcounter Learn...
RT-qPCR is the gold standard and most commonly used method for measuring gene expression. Selection of appropriate reference gene(s) normalization a crucial part experimental design, which allows accurate quantification reliability results. Because there no universal even housekeeping genes’ expression can vary under certain conditions, careful selection an internal control must be performed each cell type or tissue design. The aim this study was to identify stable genes during osteogenic...
Despite several methods that exist in different fields of life sciences, certain biotechnological applications still require microscopic analysis the samples and many instances, counting cells. Some those are drug delivery, transfection or mechanism fluorescent probes used to detect cell viability, efficiency a specific delivery some other effect. For quantification these results it is necessary either manually automatically count analyze microscope images. However, everyday use researchers...
Many studies evaluated the short-term in vitro toxicity of nanoparticles (NPs); however, long-term effects are still not adequately understood. Here, we investigated potential toxic biomedical (polyacrylic acid and polyethylenimine coated magnetic NPs) two industrial (SiO2 TiO2) NPs following different exposure protocols on physiologically models that able to differentiate: L6 rat skeletal muscle cell line biomimetic normal porcine urothelial (NPU) cells. We show cells more sensitive NP then...
Environmental or biomedical exposure to nanoparticles (NPs) can results in translocation and accumulation of NPs the brain, which lead health-related problems. have been shown induce toxicity neuronal cells through several direct mechanisms, but only a few studies also explored indirect effects NPs, consequences due neighboring NPs. In this study, we analysed possible (polyacrylic acid (PAA) coated cobalt ferrite NP, TiO2 P25 maghemite NPs) on immortalized mouse microglial differentiated CAD...
Manual cell counting in microscopic images is usually tedious, time consuming and prone to human error. Several programs for automatic have been developed so far, but most of them demand some specific knowledge image analysis and/or manual fine tuning various parameters. Even if a set filters found tuned the application, small changes attributes might make counter very unreliable. The goal this article present new application that overcomes problem by learning parameters each thus making it...
Normal porcine urothelial cells have been shown to a much lower rate of endocytosis than papillary neoplasm cells. This could be used as mechanism for selective delivery toxic compounds, such polyethyleneimine coated nanoparticles (NPs). However, these NPs induce nonselective toxicity through direct membrane disruption. can reduced by functionalization with L-glutathione or bovine serum albumin reducing their surface charge. Functionalization was confirmed Fourier Transform Infrared...