A5033099597- Muscle Physiology and Disorders
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Pesticide Exposure and Toxicity
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Nerve injury and regeneration
- Muscle activation and electromyography studies
- Gastrointestinal Tumor Research and Treatment
- Adipose Tissue and Metabolism
- Gastric Cancer Management and Outcomes
- Metastasis and carcinoma case studies
- Pain Mechanisms and Treatments
- Osteoarthritis Treatment and Mechanisms
- Sulfur Compounds in Biology
- Nutrition, Genetics, and Disease
- Colorectal and Anal Carcinomas
- Pesticide and Herbicide Environmental Studies
- Nicotinic Acetylcholine Receptors Study
- Medicinal Plants and Neuroprotection
- Proteoglycans and glycosaminoglycans research
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Computational Drug Discovery Methods
University of Ljubljana
2013-2024
Institute of Oncology Ljubljana
2000-2017
University Clinical Centre Maribor
2015
Ljubljana University Medical Centre
2008
Tallinn University of Technology
2008
Myoblast proliferation and myotube formation are critical early events in skeletal muscle regeneration. The attending inflammation cytokine signaling involved regulation of cell differentiation. Secretion muscle‐derived cytokines upon exposure to inflammatory factors may depend on the differentiation stage regenerating cells. Cultured human myoblasts myotubes were exposed 24‐hour treatment with tumor necrosis factor (TNF)‐ α or lipopolysaccharide (LPS). interleukin 6 (IL‐6), a major...
Corticosteroids are frequently used during anesthesia to provide substitution therapy in patients with adrenal insufficiency, as a first-line treatment of several life-threatening conditions, prevent postoperative nausea and vomiting, component multimodal analgesia. For these last 2 indications, dexamethasone is most used. Due the structural resemblance between aminosteroid muscle relaxants dexamethasone, concerns have been raised about possible corticosteroid inhibition reversal...
Effects of low‐load blood flow restricted (LL‐BFR) training remain unexplored in patients with ACL rupture. Our hypothesis was that LL‐BFR triggers augmented gains knee muscle strength and size, which are paralleled transcriptional responses hypoxia‐regulated genes myokines. Eighteen volunteers (age 37.5 ± 9 years) planned for reconstruction, participated the study. Twelve were divided between BFR group, performing sessions exercise, SHAM‐BFR group equal sham vascular occlusion. Six subjects...
Abstract Motor axons extending from embryonic rat spinal cord explants form fully mature neuromuscular junctions with cocultured human muscle. This degree of maturation is not observed in muscle innervated by dissociated motor neurons. Glial cells present the seem to be, besides remaining interneurons, major difference between two culture systems. In light this observation and well documented role glia neuronal development, it can be hypothesized that differentiated long‐lived vitro only if...
The aim of this study was to elucidate the mechanisms responsible for effects innervation on maturation excitation-contraction coupling apparatus in human skeletal muscle. For purpose, we compared establishment mechanism myotubes differentiated four different experimental paradigms: 1) aneurally cultured, 2) cocultured with fetal rat spinal cord explants, 3) cultured medium conditioned by cocultures, and 4) supplemented purified recombinant chick neural agrin. Ca(2+) imaging indicated that...
The cardiotonic steroids (CTS), such as ouabain and marinobufagenin, are thought to be adrenocortical hormones secreted during exercise the stress response. catalytic α-subunit of Na,K-ATPase (NKA) is a CTS receptor, whose largest pool located in skeletal muscles, indicating that muscles major target for CTS. Skeletal contribute adaptations by secreting interleukin-6 (IL-6) plethora other cytokines, which exert paracrine endocrine effects non-muscle tissues. Here, we determined whether...
A frequent finding in patients surviving critical illness myopathy is chronic muscle dysfunction. Its pathogenesis mostly unknown; one explanation could be that regeneration, which normally follows myopathy, insufficient these because of a high glucocorticoid level their blood. Glucocorticoids can prevent stimulatory effects proinflammatory factors on the interleukin (IL)-6 secretion, diminishing this way autocrine and paracrine IL-6 actions known to stimulate proliferation at earliest,...
Long term effects of different doses ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.Human myoblasts obtained from biopsies cultured irradiated with a Darpac 2000 X-ray unit at 4, 6 8 Gy. Acute by interleukin - (IL-6) release detected the heat shock protein (HSP) level, while long followed proliferation death.Compared non-irradiated control cells treated inhibitor Ara C, decreased 72 h...
Neuronal agrin, a heparan sulphate proteoglycan secreted by the α-motor neurons, promotes formation and maintenance of neuromuscular junction binding to Lrp4 activating muscle-specific kinase (MuSK). agrin also myogenesis enhancing differentiation maturation myotubes, but its effect on proliferating human myoblasts, which are often considered be unresponsive remains unclear. Using primary we determined that neuronal induced transient dephosphorylation ERK1/2, while c-Abl, STAT3, focal...
Injury of skeletal muscle is followed by regeneration in which new tissue formed from the proliferating mononuclear myoblasts, and systemic response to stress that exposes myoblasts increased glucocorticoid (GC) concentration. Because its various causes, hypoxia a frequent condition affecting muscle, therefore both processes, importantly determine outcome injury, often proceed under hypoxic conditions. It important identify characterize human myoblasts: 1) generally organized...
Previous animal and human studies have suggested that total plasma sulfide plays a role in the pathophysiology of shock. This study's aim was to determine value as marker shock severity nonsurgical adult patients admitted ICU. Forty-one patients, with various types (septic, cardiogenic, obstructive, hypovolemic), were included study, an average concentration 23.2 ± 26.3 µM. Survivors (of shock) had lower concentrations than nonsurvivors (13.0 vs. 31.9 31.5 µM; P = 0.02). Total correlated...
Sugammadex reverses neuromuscular block (NMB) through binding aminosteroid blocking agents. Although sugammadex appears to be highly selective, it can interact with other drugs, like corticosteroids. A prospective single-blinded randomized clinical trial was designed explore the significance of interactions between dexamethasone and sugammadex. Sixty-five patients who were anesthetized for elective abdominal or urological surgery included. NMB assessed using train-of-four stimulation (TOF),...
Synaptic basal lamina is interposed between the pre- and postsynaptic membrane of neuromuscular junction (NMJ). This position permits deposition lamina-bound NMJ components both neuronal muscle fibre origin. One such molecule acetylcholinesterase (AChE). The origin AChE has been investigated previously as answer would elucidate relative contributions fibers motor neurons to formation. However, in experimental models used prior investigations either or muscular NMJs were removed, poorly...
Contraction-induced adaptations in skeletal muscles are well characterized vivo, but the underlying cellular mechanisms still not completely understood. Cultured human myotubes represent an essential model system for muscle that can be modulated ex they quiescent and do contract unless being stimulated. Stimulation achieved by innervation of vitro co-culturing with embryonic rat spinal cord, or replacing motor neuron activation electrical pulse stimulation (EPS). Effects these two...
Denervation reduces the abundance of Na+,K+-ATPase (NKA) in skeletal muscle, while reinnervation increases it. Primary human muscle cells, most widely used model to study vitro, are usually cultured as myoblasts or myotubes without neurons and typically do not contract spontaneously, which might affect their ability express regulate NKA. We determined how differentiation, de novo innervation, electrical pulse stimulation expression NKA (α β) subunits regulators FXYD1 (phospholemman) FXYD5...
Abstract Background The association between hepatitis C virus (HCV) infection and lichen planus (LP) is a subject of controversy. Prevalence studies HCV in LP patients various countries reveal diverse results. Slovenian population rather homogenous with specific geographic epidemiological characteristics. Lack data or contradictory results from neighboring urged the need for case‐controlled study our patients. Methods retrospective was performed on 173 Control group included 218...
Chondrocytes in human articular cartilage remain viable post-mortem. It has however not been established yet how the storage temperature affects their survival, which is essential information when post-mortem used for toxicologic studies. Our aim was to construct a simple model of explanted knee and test influences time on viability chondrocytes ex vivo conditions. Osteochondral cylinders were procured from cadaveric femoral condyles. The embedded water-tight rubber tubes, formed separate...