In Italy, the sale of raw milk from vending machines has been allowed since 2004. Boiling treatment before its use is mandatory for consumer, because could be an important source foodborne pathogens. This study fits into this context with aim to evaluate microbiological quality 30 samples periodically collected (March 2013 July 2013) 3 located in Molise, a region southern Italy. Milk were stored 72 h at 4 °C and then subjected different treatments, such as boiling microwaving, simulate...

Lactic acid bacteria could positively affect the health of honey bees, including nutritional supplementation, immune system development and pathogen colonization resistance. Based on these considerations present study evaluated predominant Acid Bacteria (LAB) species from beebread as well social stomach midgut Apis mellifera ligustica bee foragers. In detail, for each compartment, diversity in biotypes was ascertained through multiple culture-dependent approaches, consisting Polymerase Chain...

Grape-associated microbial community is influenced by a combination of viticultural, climatic, pedological and anthropological factors, collectively known as

Chalkbrood is the manifestation of fungal disease caused by Ascosphaera apis, which affects brood developing honeybees, particularly in Apis mellifera colonies. Recently, Metschnikowia pulcherrima have been proposed as biocontrol agent winemaking and for management major postharvest soil-borne plant pathogenic fungi. In this study, antagonistic activity three M. strains against fifteen A. apis strains, isolated from contaminated hives mellifera, was evaluated, with a specific focus ona...

is an insect symbiont with documented beneficial effects on the health of honeybees. It belongs to fructophilic lactic acid bacteria (FLAB), a subgroup (LAB) notably recognized for their safe status. This fact, together its recurrent isolation from hive products that are traditionally part human diet, suggests possible use as probiotic. Our data concerning three strains

Malolactic fermentation (MLF) is a biological process that, in addition to deacidifying, also improves stability and changes the chemical sensorial characteristics of wines. However, multiple biotic abiotic factors, present must wine, make onset completion MLF by indigenous malolactic bacteria or added commercial starters difficult. This work illustrates metabolic fermentative dynamics winemaking Fiano using starter Saccharomyces cerevisiae selected strain Lactobacillus plantarum M10. In...

The COVID-19 pandemic has profoundly impacted global health, leading to extensive research focused on developing strategies enhance outbreak response and mitigate the disease’s severity. In aftermath of pandemic, attention shifted towards understanding addressing long-term health implications, particularly in individuals experiencing persistent symptoms, known as long COVID. Research into potential interventions alleviate COVID symptoms intensified, with a focus support immune function...

In our study, we dwelled upon combinations of lactobacilli/prebiotics, considering four different strains belonging to the Lactobacillus rhamnosus species, including GG (LGG), and prebiotics often found in commercial synbiotic products, such as inulin, lactulose polyols mannitol sorbitol. first step research, survival, growth kinetic parameters protein expression Lb. cultivated presence a unique carbon source were evaluated. second step, influence pre-cultivation medium added metabolizable...

Forty-two oenological strains of Lb. plantarum were assessed for their response to ethanol and pH values generally encountered in wines. Strains showed a higher variability the survival when exposed low (3.5 or 3.0) than (10 14%). The study allowed individuate highest concentration (8%) lowest value (4.0) growth strains, even if maximum specific rate (μ max ) resulted significantly reduced by these conditions. Two (GT1 LT11) preadapted 2% cultured up 14% those non-preadapted they cultivated...

The 3-Phenyllactic acid (PLA) produced by various lactic bacteria possesses a broad spectrum of antimicrobial activity. In this study, the effect PLA against Listeria innocua was studied with aim to obtain additional information about its mechanism action. pH on anti-Listeria activity investigated and pH-dependent behaviour, typical weak acid, detected. antilisterial firstly compared that than expressed phenolic acids (gallic, caffeic ferulic acids) evaluating MIC, MBC survival kinetic...

Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion wine stress conditions. Sixty-eight L. isolated from diverse sources were assessed for their ability form biofilm (pH 3.5 or 3.2) ethanol (12% 14%) The effect incubation times (24 72 h) on the formation evaluated. study highlighted that, regardless isolation source conditions, strain-dependent. Specifically, two...

In this study, 23 samples of traditional wines produced in Southern Italy were subjected to microbiological analyses with the aim identify and biotype predominant species lactic acid bacilli. For purpose, a multiple approach, consisting application both phenotypic (API 50CHL test) biomolecular methods (polymerase chain reaction-denaturing gradient gel electrophoresis 16S rRNA gene sequencing) was used. The results showed that Lactobacillus plantarum species, whereas Lb. brevis detected lower...

Abstract The effect of an antifungal culture Lactobacillus plantarum to be used in the storage at refrigeration temperature fresh black truffles was examined. strain selected among 29 lactobacilli isolated from foods and evaluated for their viability acidification activity 4 °C, as well inhibitory against 11 Penicillium strains stored temperature. Lb. showed ability hold not only growth truffles, but also that P. digitatum DSM 2750, a green mold involved spoilage truffles. observed vitro...

The present study evaluated the physico-chemical and microbiological features of Ventricina, considering for first time presence different compartments deriving from technology production. In fact meat pieces (pork muscle fat cut into cubes about 10-20cm3), mixed with other ingredients then stuffed pig bladder, are still distinguishable at end ripening. They appear delimited on outside by casing inside thin layers consisting spices (mainly red pepper powder), salt juices. Our results showed...

The present study aimed to find solutions based on the use of plant-based ingredients that would improve nutritional quality meat products as well ensure sensory and microbiological quality. Two fat replacers, lemon albedo (Citrus lemon) carob seed gum (Ceratonia siliqua), were investigated by chemical analysis panel testing evaluate their effect beef burgers. antimicrobial activity two plant extracts, from nettle (Urtica dioica) leaves medlar (Eriobotrya japonica) seeds, was studied,...

In the present study, a multiple approach was used to characterize Malpighia punicifolia extract and evaluate its inhibitory activity against several meat spoilage bacteria. First, volatile fraction, vitamins phenolic compounds of obtained by supercritical fluid extraction were determined GC-MS HPLC. Then, antimicrobial action in vitro evaluated Pseudomonas putida DSMZ 291(T), fluorescens 50009(T), fragi 3456(T), Brochothrix thermosphacta 20171(T) agar well diffusion assay dilution test....

An Universal Stress Protein (USP) expressed under acid stress condition by Listeria innocua ATCC 33090 was investigated. The USP up-regulated not only in the stationary phase but also during exponential growth phase. three dimensional (3D) structure of predicted using a combined proteomic and bioinformatics approach. Phylogenetic analysis showed that from detected our study distant USPs other bacteria (such as Pseudomonas spp., Escherichia coli, Salmonella spp.) clustered separate...

Many probiotic formulations are available in the market and advertised for several preventive or curative roles. The aim of this study was identification microorganisms composing different lactic acid bacteria based pharmaceutical ascertainment their ability to survive gastro-intestinal (GI) stresses, main requisite produce beneficial effects. For purpose, viable were enumerated by plate counts media. Denaturing Gradient Gel Electrophoresis-Polymerase Chain Reaction (PCR-DGGE) analysis...