A5101649201- Vasculitis and related conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sarcoidosis and Beryllium Toxicity Research
- Wnt/β-catenin signaling in development and cancer
- Otitis Media and Relapsing Polychondritis
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Hemoglobin structure and function
- Complement system in diseases
- Autoimmune Bullous Skin Diseases
- Luminescence and Fluorescent Materials
- Renal Diseases and Glomerulopathies
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Atherosclerosis and Cardiovascular Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tendon Structure and Treatment
- Fibroblast Growth Factor Research
- Mast cells and histamine
- Hydrogen's biological and therapeutic effects
- PI3K/AKT/mTOR signaling in cancer
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Polydiacetylene-based materials and applications
University of North Carolina at Chapel Hill
2011-2025
University of British Columbia
2023-2024
University of Minnesota System
2019
Arkana Laboratories
2019
Ludong University
2015
Indiana University School of Medicine
2005
University of Iowa
1995
Antineutrophil cytoplasmic autoantibodies (ANCAs) are identified in the circulation of approximately 80% patients with pauci-immune necrotizing and crescentic glomerulonephritis systemic small vessel vasculitis, such as microscopic polyangiitis Wegener granulomatosis. The most common antigen target for ANCAs is myeloperoxidase (MPO), which found neutrophils monocytes. We report definitive experimental animal evidence that pathogenic. MPO knockout (Mpo(-/-)) mice were immunized mouse MPO....
Antineutrophil cytoplasmic autoantibodies (ANCAs) are identified in the circulation of approximately 80% patients with pauci-immune necrotizing and crescentic glomerulonephritis systemic small vessel vasculitis, such as microscopic polyangiitis Wegener granulomatosis. The most common antigen target for ANCAs is myeloperoxidase (MPO), which found neutrophils monocytes. We report definitive experimental animal evidence that pathogenic. MPO knockout (Mpo–/–) mice were immunized mouse MPO....
Necrotizing and crescentic GN (NCGN) with a paucity of glomerular immunoglobulin deposits is associated ANCA. The most common ANCA target antigens are myeloperoxidase (MPO) proteinase 3. In manner that requires activation the alternative complement pathway, passive transfer antibodies to mouse MPO (anti-MPO) induces model NCGN closely mimics human disease. Here, we confirm importance C5aR/CD88 in mediation anti-MPO-induced report C6 not required. We further demonstrate deficiency C5a-like...
In the human autoimmune blistering disease pemphigus vulgaris (PV) pathogenic antibodies bind desmosomal cadherin desmoglein-3 (dsg3), causing epidermal cell-cell detachment (acantholysis). Pathogenic PV dsg3 autoantibodies were used to initiate desmosome signaling in keratinocyte cell cultures. Heat shock protein 27 (HSP27) and p38MAPK identified as proteins rapidly phosphorylated response IgG. Inhibition of activity prevented IgG-induced HSP27 phosphorylation, keratin filament retraction,...
Pemphigus vulgaris (PV) is a life-threatening autoimmune blistering skin disease characterized by detachment of keratinocytes (acantholysis). It has been proposed that PV IgG might trigger signaling and this process may lead to acantholysis. Indeed, we recently identified rapid dose-dependent phosphorylation p38 mitogen-activated protein kinase (p38MAPK) heat shock (HSP) 27 after binding antibodies cultured keratinocytes. In human keratinocyte cultures, inhibitors p38MAPK prevented...
ConspectusThermally activated delayed fluorescence (TADF) materials are widely used in organic light-emitting diodes, but their long emission lifetimes also make them ideal for use bioimaging probes, fluorescent sensors, and phototheranostics. Unfortunately, development toward these applications has been restricted by the poor compatibility of most TADF with aqueous conditions. This problem can be addressed encapsulating dyes into nanoparticles that form stable suspensions, while...
Antineutrophil cytoplasmic autoantibodies (ANCAs) are identified in the circulation of approximately 80% patients with pauci-immune necrotizing and crescentic glomerulonephritis systemic small vessel vasculitis, such as microscopic polyangiitis Wegener granulomatosis. The most common antigen target for ANCAs is myeloperoxidase (MPO), which found neutrophils monocytes. We report definitive experimental animal evidence that pathogenic. MPO knockout (Mpo–/–) mice were immunized mouse MPO....
Drug-induced autoimmune diseases are increasingly recognized although mechanistic insight into disease causation is lacking. Hydralazine exposure has been linked to diseases, including anti-neutrophil cytoplasmic autoantibody (ANCA) vasculitis. Our hypothesis posits that hydralazine covalently binds myeloperoxidase (MPO), triggering the response in ANCA We vitro observed formation of carbonyl derivatives on amine groups presence acrolein. This facilitated subsequent binding heme-containing...
There is accumulating evidence that complement activation important in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) pathogenesis. This study was undertaken to investigate AAV with myeloperoxidase (MPO) positivity and proteinase 3 (PR3) after determining optimal methods for measuring activated factors circulation.Participants included 98 patients (45 MPO-ANCA positive, 53 PR3-ANCA positive) 35 healthy controls. Plasma obtained from blood collected using EDTA tubes,...
Application of adeno-associated virus (AAV) vector in large animal studies and clinical trials often requires high-titer high-potency vectors. A number currently used production methods, based on either transient transfection or helper infection cell lines, have their advantages limitations. We previously developed a 293-cell-based producer line method for AAV2 Similar to several other however, it multiple cloning steps the packaging plasmids two-step selection stable lines. Here we report...
Luminescent glassy organic dots (g-Odots) are emerging as promising nanoparticles for bioimaging, exhibiting excellent brightness and photostability attributed to their glassy, rigid matrix. Emission color tuning in g-Odots is also easily accessible, since different hydrophobic fluorophores can be encapsulated within the matrix of particle. particularly useful when combined with dyes phosphorescence or thermally activated delayed fluorescence (TADF), facilitating time-gated imaging while...
<i>O</i>-Glycosylation modifies and regulates a variety of intracellular proteins. Plakoglobin, which functions in both cell-cell adhesion signal transduction, is modified by <i>O</i>-glycosylation; however, the significance unknown. To investigate functional consequence plakoglobin <i>O</i>-glycosylation, we cloned overexpressed keratinocytes murine <i>O</i>-GlcNAc transferase (mOGT). Over expression mOGT resulted (i) glycosylation (ii) increased levels due to post-translational...
The most promising strategies to overcome bacterial drug resistance are the use of nanoparticles (NPs). In this paper, extract Gynostemma pentaphyllum Makino was prepared and used as reducing well stabilising agent obtain silver NPs (AgNPs). active phytochemicals present in herb were responsible for quick reduction ion (Ag+) metallic AgNPs (Ag0). method is green rapid, whole synthesis process can be finished within 15 min at 60°C. An intense surface plasmon resonance band 420–450 nm range...
Myeloperoxidase-specific ANCA (MPO-ANCA) are implicated in the pathogenesis of vasculitis and GN. Kinins play a major role during acute inflammation by regulating vasodilatation vascular permeability modulating adhesion migration leukocytes. Kinin system activation occurs patients with vasculitis. Previous studies animal models GN sclerosing kidney diseases have demonstrated protective effects bradykinin receptor 1 (B1R) blockade