A5100382271- Metabolism, Diabetes, and Cancer
- Melanoma and MAPK Pathways
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Pancreatic function and diabetes
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Silk-based biomaterials and applications
- dental development and anomalies
- Protein Kinase Regulation and GTPase Signaling
- Bone and Dental Protein Studies
- melanin and skin pigmentation
- Skin Protection and Aging
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- PI3K/AKT/mTOR signaling in cancer
- Periodontal Regeneration and Treatments
- TGF-β signaling in diseases
- interferon and immune responses
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
University College Dublin
2022-2023
Xuan Wu Hospital of the Capital Medical University
2022
First Affiliated Hospital of Xi'an Jiaotong University
2020
Institute of Molecular and Cell Biology
2012
Indiana University School of Medicine
2005
Hospital for Sick Children
1993-2002
Like neuronal synaptic vesicles, intracellular GLUT4-containing vesicles must dock and fuse with the plasma membrane, thereby facilitating insulin-regulated glucose uptake into muscle fat cells. GLUT4 colocalizes in part vesicle SNAREs VAMP2 VAMP3. In this study, we used a single-cell fluorescence-based assay to compare functional involvement of VAMP3 translocation. Transient transfection proteolytically active tetanus toxin light chain cleaved both proteins L6 myoblasts stably expressing...
DNA damage and the elicited cellular response underlie etiology of tumorigenesis ageing. Yet, how this integrates inputs from cells' environmental cues remains underexplored. Here we report that BMP-Smad1 pathway, which is essential for embryonic development tissue homeostasis, has an important role in oncogenesis. On genotoxic stress, Atm phosphorylates BMPs-activated Smad1 nucleus on S239, disrupts interaction with protein phosphatase PPM1A, leading to enhanced activation upregulation...
There is a need to understand whether the amount of GLUT4 at cell surface determines extent glucose uptake in response insulin. Thus, we created heterozygous mouse expressing modest levels myc-tagged (GLUT4myc) insulin-sensitive tissues under control human promoter. Insulin stimulated 2-deoxyglucose 6.5-fold isolated brown adipocytes. GLUT1 did not contribute insulin response. The stimulation by was completely blocked wortmannin and partly (55 ± 2%) p38 mitogen-activated protein kinase...
Kinase Suppressor of RAS 1 (KSR1) is a scaffolding protein for the RAS-RAF-MEK-ERK pathway, which one most frequently altered pathways in human cancers. Previous results have shown that KSR1 has critical role mutant RAS-mediated transformation. Here, we examined BRAF We used CRISPR/Cas9 to knock out BRAFV600E-transformed melanoma cell line. loss produced complex phenotype characterised by impaired proliferation, cycle defects, decreased transformation, invasive migration, increased cellular...
Abstract KSR1 is a scaffolding protein for the RAS-RAF-MEK-ERK pathway, which one of most frequently altered pathways in human cancers. Previous results have shown that has critical role mutant RAS mediated transformation. Here, we examined BRAF We used CRISPR/Cas9 to knock out BRAFV600E transformed melanoma cell line. loss produced complex phenotype characterized by impaired proliferation, cycle defects, decreased transformation, invasive migration, increased cellular senescence, and...
With the increasing emphasis on use of nonanimal ingredients in clinical care, studies have proposed TrypLE™ as an alternative to trypsin. However, previous research has reported insufficient cell yield and viability when using TrypLE isolate skin cells compared dispase/trypsin-EDTA method. This study aimed propose improved method for isolated by evaluate keratinocytes melanocytes. Foreskin tissues were melanocytes trypsin-EDTA protocol our modified protocol. The freshly compared, epidermal...
Induced pluripotent stem (iPS) cells could be induced into ameloblast-like by ameloblasts serum-free conditioned medium (ASF-CM), and bone morphogenetic proteins (BMPs) might essential during the regulation of this process. The present study investigates signal transduction that regulates ameloblastic differentiation iPS ASF-CM. Mouse were characterized then cultured for 14 days in epithelial cell (control) or Bone protein receptor II (BMPR-II) siRNA, inhibitor Smad1/5 phosphorylation...
Abstract Background This study aims to explore the expression of circRNA-0008302 in adipose stem cells derived exosome (ADSCs-Exo), and investigate function as well mechanism H 2 O -induced cardiomyocyte injury. Methods Differentially expressed circRNAs between ADSCs ADSCs-Exo were identified by circRNA chip screening, further validated qRT-PCR. CircRNA-0008302 siRNA was used silence M6200 mouse cardiac myocytes. divided into four groups: group, + group circ−0008302 group. Thereafter, cell...