A5047475750- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- TGF-β signaling in diseases
- Chronic Myeloid Leukemia Treatments
- FOXO transcription factor regulation
- Hippo pathway signaling and YAP/TAZ
- Reproductive System and Pregnancy
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer, Lipids, and Metabolism
- Immune Response and Inflammation
- Kruppel-like factors research
- Renal and related cancers
- Peroxisome Proliferator-Activated Receptors
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Hedgehog Signaling Pathway Studies
University of Washington
2018-2024
University of Ottawa
2014-2018
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2018
Shanghai University of Traditional Chinese Medicine
2018
Shanghai Jiao Tong University
2012-2016
Singapore Eye Research Institute
2015
Institute of Molecular and Cell Biology
2009-2015
Shandong University
2008-2013
Ministry of Education of the People's Republic of China
2012
Agency for Science, Technology and Research
2010-2012
Abstract There is increasing evidence that self‐renewal capacity of cancer cells critical for carcinogenesis; hence, it vital to examine the expression and involvement regulatory genes in these cells. Here, we reported Oct4, a well‐known regulator embryonic stem cells, was highly expressed human gliomas glioma cell lines, levels were increased parallel with grades. In vitro cultures, Oct4 only rat C6 neural but not brain differentiated Downregulation by RNA interference associated reduced...
Abstract Stromal cells, infiltrating immune paracrine factors and extracellular matrix have been extensively studied in cancers. However, autocrine produced by tumor cells communications between intracellular signaling pathways the development of drug resistance, cancer stem-like (CSCs) tumorigenesis not well investigated, precise mechanism tangible approaches remain elusive. Here we reveal a new which cytokines breast after chemotherapy withdrawal activate both Wnt/ β -catenin NF- κ B...
Cancer stem cells (CSCs) are responsible for tumor initiation and progression. Toll-like receptors (TLRs) highly expressed in cancer associated with poor prognosis. However, a linkage between CSCs TLRs is unclear, potential intervention strategies to prevent TLR stimulation-induced CSC formation underlying mechanisms lacking. Here, we demonstrate that stimulation of toll-like receptor 3 (TLR3) promotes breast toward phenotype vitro vivo. Importantly, conventional NF-κB signaling pathway not...
The mechanisms underlying disparate roles of the canonical Wnt signaling pathway in maintaining self-renewal or inducing differentiation and lineage specification embryonic stem cells (ESCs) are not clear. In this study, we provide first demonstration that versus human ESCs (hESCs) response to is predominantly determined by a two-layer regulatory circuit involving β-catenin, E-cadherin, PI3K/Akt, Slug time-dependent manner. Short-term upregulation β-catenin does lead activation T-cell factor...
// Deyong Jia 1 , Yuan Tan Huijuan Liu 1, 2 Sarah Ooi Li Kathryn Wright Steffany Bennett Christina L. Addison 3 Lisheng Wang 4, 5 Department of Biochemistry, Microbiology and Immunology, Faculty Medicine, University Ottawa, Ontario K1H 8M5, Canada Bio-X Institutes, Key Laboratory for the Genetics Developmental Neuropsychiatric Disorders, Ministry Education, Shanghai Jiao Tong University, 200240, China Centre Cancer Therapeutics, Ottawa Hospital Research Institute, 8L6, 4 Regenerative...
Triple‐negative breast cancer ( TNBC ), the most refractory subtype of to current treatments, accounts disproportionately for majority cancer‐related deaths. This is largely due plasticity and development stem cells CSC s). Recently, distinct yet interconvertible mesenchymal‐like epithelial‐like states have been revealed in s. Thus, strategies capable simultaneously inhibiting bulk populations both mesenchymal epithelial be developed. Wnt/β‐catenin Hippo/ YAP pathways are crucial...
DNA damage triggers a network of signaling events that leads to cell cycle arrest or apoptosis. This response acts as mechanism prevent cancer development. It has been reported fatty acids (FAs) synthesis is increased in many human tumors while inhibition acid synthase (FASN) could suppress tumor growth. Here we report saturated (SFAs) play negative role response. Palmitic acid, well stearic and myristic compromised the induction p21 Bax expression double stranded breaks ssDNA, knockdown...
Neural stem cells (NSCs) play essential roles in nervous system development and postnatal neuroregeneration their deregulation underlies the of neurodegenerative disorders. Yet how NSC proliferation differentiation are controlled is not fully understood. Here we present evidence that tumor suppressor p53 regulates via bone morphogenetic proteins (BMP)-Smad1 pathway its target gene inhibitor DNA binding 1 (Id1). deficiency led to increased neurogenesis vivo, biased neuronal augmented ex vivo...
DNA damage and the elicited cellular response underlie etiology of tumorigenesis ageing. Yet, how this integrates inputs from cells' environmental cues remains underexplored. Here we report that BMP-Smad1 pathway, which is essential for embryonic development tissue homeostasis, has an important role in oncogenesis. On genotoxic stress, Atm phosphorylates BMPs-activated Smad1 nucleus on S239, disrupts interaction with protein phosphatase PPM1A, leading to enhanced activation upregulation...
Abstract Cancer-associated fibroblasts (CAFs) mediate an immunosuppressive effect, but the underlying mechanism remains incompletely defined. Here we show that increasing prostatic stromal Foxf2 suppresses growth and progression of both syngeneic autochthonous mouse prostate cancer models in immunocompetent context. Mechanistically, moderately attenuates CAF phenotype transcriptionally downregulates Cxcl5 , which diminish myeloid cells enhance T cell cytotoxicity. Increasing sensitizes to...
Abstract The organization of mammalian genomes within the nucleus features a complex, multiscale three-dimensional (3D) architecture. functional significance these 3D genome features, however, remains largely elusive due to limited single-cell technologies that can concurrently profile and transcriptional activities. Here, we report GAGE-seq, highly scalable, robust co-assay simultaneously measures structure transcriptome same cell. Employing GAGE-seq on mouse brain cortex human bone marrow...
Protein palmitoylation regulates many aspects of cell function and is carried out by acyl transferases that contain zf-DHHC motifs. The in vivo physiological protein largely unknown. Here we generated mice deficient the transferase Aph2 (Ablphilin 2 or zf-DHHC16) demonstrated an essential role for embryonic/postnatal survival, eye development, heart development. Aph2(-/-) embryos pups showed cardiomyopathy cardiac defects including bradycardia. We identified phospholamban, a often associated...
Tumor suppressor p53, which is activated by various stress and oncogene activation, a target for anti-cancer drug development. In this study, screening panels of protein kinase inhibitors phosphatase inhibitors, we identified 5-Iodotubercidin as strong p53 activator. purine derivative used an inhibitor kinases including adenosine kinase. We found that could cause DNA damage, verified induction breaks nuclear foci positive γH2AX TopBP1, activation Atm Chk2, S15 phosphorylation up-regulation...
"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on have been directed toward biomarker discovery for diagnosis, response treatment, mechanisms. This study employed spontaneously immortalized normal human conjunctival line, IOBA-NHC, as model investigate hyperosmotic stress-induced changes of metabolites proteins. Global targeted metabonomic analyses well...
The molecules and mechanisms pertinent to the low immunogenicity of undifferentiated embryonic stem cells (ESCs) remain poorly understood. Here, we provide evidence that milk fat globule epidermal growth factor 8 (MFG-E8) is a vital mediator in this phenomenon directly suppresses T cell immune responses. MFG-E8 enriched ESCs but diminished differentiated ESCs. Upregulation increases successful engraftment both across major histocompatibility complex barriers. activation/proliferation...
Abstract The phenotypic and functional heterogeneity of the mouse prostate epithelial cell lineages remains incompletely characterized. We show that Sca-1+ luminal cells at proximal express Sox2. These are replicative quiescent, castration resistant, do not possess secretory function. use Probasin-CreERT2 Sox2-CreERT2 models in concert with a fluorescent reporter line to label Sca-1− cells, respectively. By lineage tracing approach, we two populations independently sustained. Sox2 is...