A5100454565- Advanced biosensing and bioanalysis techniques
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Crustacean biology and ecology
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Regulation of Appetite and Obesity
- Aquaculture disease management and microbiota
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- Ethics and Legal Issues in Pediatric Healthcare
- melanin and skin pigmentation
- Tumors and Oncological Cases
- Cancer-related gene regulation
- Heat shock proteins research
- Reproductive biology and impacts on aquatic species
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- MicroRNA in disease regulation
Peking University
2008-2025
Chengdu Second People's Hospital
2025
Fujian Provincial People's Hospital
2024
Fujian Provincial Hospital
2022-2024
East China Normal University
2013-2024
Chungnam National University
2024
The University of Texas Southwestern Medical Center
2023
First Affiliated Hospital of Xi'an Jiaotong University
2023
Mianyang Central Hospital
2023
Hunan Agricultural University
2022
A new approach to ultrasensitive detection of DNA hybridization based on nanoparticle-amplified surface plasmon resonance (SPR) is described. Use the Au nanoparticle tags leads a greater than 10-fold increase in angle shift, corresponding more 1000-fold improvement sensitivity for target oligonucleotide as compared unamplified binding event. This enhanced shift SPR reflectivity combined result greatly increased mass, high dielectric constant particles, and electromagnetic coupling between...
One manifestation of fluorescence resonance energy transfer (FRET) is an increase in donor after photobleaching the acceptor. Published acceptor-photobleaching methods for FRET have mainly used wide-field microscopy. A laser scanning confocal microscope enables faster and targeted bleaching within field view, thereby improving speed accuracy. Here we demonstrate approach with CFP YFP, most versatile fluorescent markers now available FRET. CFP/YFP imaging has been accomplished a single...
Integrin alpha(v)beta(3) plays a critical role in tumor angiogenesis and metastasis. Suitably radiolabeled cyclic arginine-glycine-aspartic (RGD) peptides can be used for noninvasive imaging of expression targeted radionuclide therapy. In this study, we developed (64)Cu-labeled multimeric RGD peptides, E{E[c(RGDyK)](2)}(2) (RGD tetramer) E(E{E[c(RGDyK)](2)}(2))(2) octamer), PET integrin expression.Both tetramer octamer were synthesized with glutamate as the linker. After conjugation...
mahoganoid is a mouse coat-color mutation whose pigmentary phenotype and genetic interactions resemble those of Attractin (Atrn). Atrn mutations also cause spongiform neurodegeneration. Here, we show that null for causes similar age-dependent neuropathology includes many features prion diseases but without accumulation protease-resistant protein. The gene mutated in encodes RING-containing protein with E3 ubiquitin ligase activity vitro. Similarities phenotype, expression, suggest genes are...
One-dimensional Ni/Ni3C core-shell nanoball chains with an average diameter by around 30 nm were synthesized means of a mild chemical solution method using soft template trioctylphosphine oxide (TOPO). It was revealed that the uniform Ni nanochains capped Ni3C thin shells about 1-4 in thickness and each core consists polygrains. The coercivity is much enhanced (600 Oe at 5 K) comparable single nanowires due to one-dimensional shape anisotropy. Deriving from distinctive structure 3C shell,...
A novel signal amplification strategy for electrochemical detection of DNA and proteins based on the amplification-by-polymerization concept is described. Specifically, a controlled radical polymerization reaction triggered after capture target molecules electrode surface. Growth long chain polymeric materials provides numerous sites subsequent aminoferrocene coupling, which in turn significantly enhances output. Activators generated electron transfer atom (AGET ATRP) used this study its...
Summary The replication of CoIE1‐related plasmids is controlled by an unstable antisense RNA, RNAI, which can interfere with the successful processing RNAII primer replication. We show here that a host protein, PcnB, supports promoting decay RNAI. In bacterial strains deleted for pcnB stable, active form RNAI*, appears to be identical product 5′‐end RNAse E, accumulates. This leads reduction in plasmid copy number. show, using GST‐ PcnB fusion does not RNAI/RNAII binding vitro. like has...
We report here a new DNA detection method in which polymer growth atom transfer radical polymerization (ATRP) is used as means to amplify signals. In this method, hybridization and ligation reactions led the attachment of ATRP initiators on solid surface where specific sequences were located. These subsequently triggered poly(hydroxyethyl methacrylate) (PHEMA) at end immobilized molecules formed brushes. The formation PHEMA altered substrate opacity, rendering corresponding spots readily...
A label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods is demonstrated. Different capture probes were encoded by the different reflectivities of Au and Ag stripe patterns. Successful induced an optically detectable conformational change in polythiophene derivatives from forming single-stranded DNA−polymer complexes double-stranded ones. The results show attomole sensitivity single-mutation...
The accessory sex gland (ASG) is an important component of the male reproductive system, which functions to enhance fertility spermatozoa during reproduction. Certain proteins secreted by ASG are known bind membrane and affect its function. gene expression profile in Chinese mitten crab (Eriocheir sinensis) has not been extensively studied, limited genetic research conducted on this species. advent high-throughput sequencing technologies enables generation genomic resources within a short...
Neural stem cells (NSCs) play essential roles in nervous system development and postnatal neuroregeneration their deregulation underlies the of neurodegenerative disorders. Yet how NSC proliferation differentiation are controlled is not fully understood. Here we present evidence that tumor suppressor p53 regulates via bone morphogenetic proteins (BMP)-Smad1 pathway its target gene inhibitor DNA binding 1 (Id1). deficiency led to increased neurogenesis vivo, biased neuronal augmented ex vivo...
Rectal schwannoma (RS) is a rare subtype of that presents diagnostic challenges owing to its clinical rarity. The absence typical symptoms, specific signs, and distinctive radiographic findings often hinders clinicians from reaching definitive diagnosis before surgical intervention. Herein, we report case RS who underwent complete resection through endoscopic full-thickness (EFTR) discuss the clinical, imaging, pathological features for differential diagnosis. A 71-year-old Chinese woman...
Radiotherapy (RT) is a primary treatment for breast cancer, but its effectiveness often compromised by hypoxia and intrinsic resistance mechanisms. The F-box protein JFK overexpressed in cancer associated with reduced radiosensitivity, specific inhibitors are currently unavailable. Herein, we developed spherical nanoparticles (SNP-JC) designed to co-deliver small interfering RNA targeting catalase the tumor, aiming silence alleviate overcome RT resistance. Positron emission tomography...
A double-hydrophilic multiarm hyperbranched polymer with a poly(amidoamine) (HPAMAM) core and many poly(ethylene glycol) monomethyl ether (MPEG) arms connected by pH-sensitive acylhydrazone bonds (HPAMAM-g-MPEG) was successfully prepared. Benefiting from the cationic dendritic PEGylation shell, used as nanoreactor for CdS quantum dots (CdS QDs) synthesis in aqueous solution. The obtained HPAMAM-g-MPEG CdS/HPAMAM-g-MPEG nanocomposites were carefully characterized 1H NMR, 13C Fourier transform...
Mutations that affect the balance between synthesis of eumelanin and pheomelanin provide a powerful set tools with which to understand general aspects cell signaling. Previous work from our laboratory has demonstrated is triggered by ability Agouti protein inhibit signaling through Melanocortin 1 receptor (Mc1r). In bioassay based on Xenopus Mc1r, two effects, competitive inhibition occupancy α‐MSH down‐regulation signaling, are mediated separately domains in amino‐ carboxy‐terminal regions...
Amplification-by-Polymerization reportedly offers a sensitive and detector-free approach for DNA detection. However, the requirement an oxygen-free environment when classic radical polymerization reactions are used in signal amplification significantly limits mobility of this point-of-need applications. We report here employment purge-free controlled/"living" reaction, activators generated by electron transfer atom (AGET ATRP), to achieve upon hybridization. Its aptitude simplifying assay...