A5035104823- Cardiomyopathy and Myosin Studies
- Force Microscopy Techniques and Applications
- Muscle Physiology and Disorders
- Genetic Neurodegenerative Diseases
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Ion Channels and Receptors
- RNA Interference and Gene Delivery
- Nicotinic Acetylcholine Receptors Study
- Plant Virus Research Studies
- Telomeres, Telomerase, and Senescence
- Wind Turbine Control Systems
- Photoreceptor and optogenetics research
- Mesenchymal stem cell research
- Energy Load and Power Forecasting
- Immune Response and Inflammation
- Transgenic Plants and Applications
- Advanced Machining and Optimization Techniques
- Hearing Loss and Rehabilitation
- thermodynamics and calorimetric analyses
- Wind Energy Research and Development
- Advanced machining processes and optimization
University of Nevada, Reno
2020-2024
R.V. College of Engineering
2022
Massachusetts General Hospital
2004-2019
Tufts University
2008-2009
Harvard University
2004
Here we describe a novel method for imaging apoptosis in cells using near-infrared fluorescent (NIRF) probe selective caspase-1 (interleukin 1beta-converting enzyme, ICE). This biocompatible, optically quenched ICE-NIRF incorporates peptide substrate, which can be selectively cleaved by caspase-1, resulting the release of fluorescence signal. The specificity this is supported various lines evidence: 1) activation purified but not another caspase vitro; 2) infection with herpes simplex virus...
Huntington's disease is initiated by the expression of a CAG repeat-encoded polyglutamine region in full-length huntingtin, with dominant effects that vary continuously size. The mechanism could involve simple gain function or more complex coupled to loss (e.g. negative-graded function). To distinguish these alternatives, we compared genome-wide gene changes correlated size across an allelic series heterozygous knock-in mouse embryonic stem (ES) cell lines (Hdh(Q20/7), Hdh(Q50/7),...
Molecular motors such as kinesin and myosin often work in groups to generate the directed movements forces critical for many biological processes. Although much is known about how individual force movement, surprisingly, little mechanisms underlying macroscopic mechanics generated by multiple motors. For example, observation that a saturating number, N, of heads move an actin filament at rate influenced actin-myosin attachment detachment kinetics accounted neither experimentally nor...
Abstract Although the synaptogenic program for cholinergic synapses of neuromuscular junction is well known, little known identity or dynamic expression patterns proteins involved in non‐neuromuscular nicotinic synapse development. We have previously demonstrated abnormal presynaptic terminal morphology following loss acetylcholine receptor (nAChR) α9 subunit adult cochleae. However, molecular mechanisms underlying these changes remained obscure. To better understand formation and role...
Rare individuals with inactivating mutations in the Huntington's disease gene (HTT) exhibit variable abnormalities that imply essential HTT roles during organ development. Here we report phenotypes produced when increasingly severe hypomorphic murine orthologue Htt, (HdhneoQ20, HdhneoQ50, HdhneoQ111), were placed over a null allele (Hdhex4/5). The most failed to rescue lethality at gastrulation, while intermediate, though still severe, alleles yielded recessive perinatal and variety of fetal...
DNA vaccines promote immune system activation in small animals and exhibit certain advantages when compared to conventional recombinant protein vaccines. However clinical trials are less effective inducing potent responses due the low delivery efficiency expression of antigens. Currently, various devices such as gene-guns, bioinjectors electroporation systems being used order increase potency However, optimal parameters required must be carefully set obtain highest levels gene strong humans....
Accurate models of muscle contraction are necessary for understanding performance and the molecular modifications that enhance it (e.g., therapeutics, posttranslational modifications, etc.). As a thermal system containing millions randomly fluctuating atoms on scale fiber generate unidirectional force power output, mechanics constrained by laws thermodynamics. According to thermodynamic model, muscle's stroke occurs with shortening an entropic spring consisting ensemble force-generating...
ABSTRACT With implications for both clean energy technologies and human health, models of muscle contraction provide insights into the inner workings one most energy-efficient engines on planet modifications to that engine lead diseases. However, only scientific methods can these insights. A binary mechanical model is a recently developed thermodynamic implies novel entropic kinetic formalism, provides solution paradox has perplexed scientists over century, accounts many energetic aspects...
Abstract Rare individuals with hypomorphic inactivating mutations in the Huntington’s Disease (HD) gene ( HTT ), identified by CAG repeat expansion eponymous neurodegenerative disorder, exhibit variable abnormalities that imply essential roles during organ development. Here we report phenotypes produced when increasingly severe Htt , murine orthologue (in Hdh neoQ20 neoQ50 neoQ111 mice), were placed over a null allele ex4/5 ). The most failed to rescue lethality at gastrulation, while...