A5092006730- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- HER2/EGFR in Cancer Research
- Mechanisms of cancer metastasis
- Calcium signaling and nucleotide metabolism
- Erythrocyte Function and Pathophysiology
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
University of Stuttgart
2023-2025
Institute of Cell Biology
2023
Protein kinase D3 (PKD3) is an important regulator of triple-negative breast cancer (TNBC) progression by promoting invasion, proliferation, and stem cell maintenance. However, the mechanism underlying these cellular functions has remained unclear. Here, we report that endogenous PKD3 localizes to Rab7/Lamp1-positive vesicles in MDA-MB-231 cells cultured on stiff matrices. Notably, upon depletion size Rab7-positive smaller. This correlates with impaired endosomal acidification, which...
The protein kinase D (PKD) family members regulate the fission of cargo vesicles at Golgi complex and play a pro-oncogenic role in triple-negative breast cancer (TNBC). Whether PKD facilitates secretion tumor-promoting factors TNBC, however, is still unknown. Using pharmacological inhibition activity siRNA-mediated depletion PKD2 PKD3, we identified PKD-dependent secretome TNBC cell lines MDA-MB-231 MDA-MB-468. Mass spectrometry-based proteomics antibody-based assays revealed significant...
The Protein kinase D (PKD) family members regulate the fission of cargo vesicles at Golgi complex and play a pro-oncogenic role in triple-negative breast cancer (TNBC). Whether PKD promotes secretion tumor-promoting factors TNBC, however, is still unknown. Here, by using pharmacological inhibition activity siRNA-mediated depletion PKD2 PKD3 we identified PKD-dependent secretome TNBC cell lines MDA-MB-231 MDA-MB-468. Mass spectrometry-based proteomics antibody-based assays revealed that...