A5103041464- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Barrier Structure and Function Studies
- Aluminum Alloy Microstructure Properties
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Traditional Chinese Medicine Analysis
- Immune Cell Function and Interaction
- Acute Ischemic Stroke Management
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Circadian rhythm and melatonin
- Nuclear Receptors and Signaling
- Circular RNAs in diseases
- Adenosine and Purinergic Signaling
- Neurological Disorders and Treatments
- Alkaloids: synthesis and pharmacology
- Microstructure and Mechanical Properties of Steels
- Metallurgical Processes and Thermodynamics
- Nutritional Studies and Diet
- Chronic Disease Management Strategies
- Cancer-related molecular mechanisms research
Nanjing University
2017-2025
Nanjing Drum Tower Hospital
2018-2025
University of Chinese Academy of Sciences
2025
Inner Mongolia University of Science and Technology
2023-2024
Army Medical University
2021-2024
Nanjing Brain Hospital
2022-2024
Southwest Hospital
2021-2024
Jiangsu Province Blood Center
2020-2023
ShanghaiTech University
2023
Jiangsu Provincial Hospital of Traditional Chinese Medicine
2023
Microglia is the major contributor of post-stroke neuroinflammation cascade and crucial cellular target for treatment ischemic stroke. Currently, endogenous mechanism underlying microglial activation following stroke remains elusive. Serglycin (SRGN) a proteoglycan expressed in immune cells. Up to now, role SRGN on largely unexplored.
Activation of microglial cells plays an important role in neuroinflammation after ischemic stroke. Inhibiting the activation has been suggested as a potential therapeutic approach treatment stroke.Oxygen-glucose deprivation primary and transient middle cerebral artery occlusion (MCAO) C57BL/6 mice were used vitro vivo stroke models. Microarray analysis was performed to investigate overall impact long non-coding RNAs (lncRNAs) on inflammation status cells. RT-qPCR evaluate lncRNA levels mRNA...
Abstract Ischemic stroke leads to white matter damage and neurological deficits. However, the characteristics of injury repair after are unclear. Additionally, precise molecular communications between microglia during rehabilitation phase remain elusive. In this current study, MRI DTI scan immunofluorescence staining were performed trace in mouse transient middle cerebral artery occlusion (tMCAO) model. We found that most serious was on Day 7 ischemic stroke, then it recovered gradually from...
Abstract Background Damage in the ischemic core and penumbra after stroke affects patient prognosis. Microglia immediately respond to insult initiate immune inflammation, playing an important role cellular injury stroke. However, microglial heterogeneity mechanisms involved remain unclear. Methods We first performed single-cell RNA-sequencing (scRNA-seq) spatial transcriptomics (ST) on middle cerebral artery occlusion (MCAO) mice from three time points determine stroke-associated subclusters...
Chronic cerebral ischemia induces white matter injury (WMI) contributing to cognitive decline. Both astrocytes and microglia play vital roles in the demyelination remyelination processes, but underlying mechanism remains unclear. This study aimed explore influence of chemokine CXCL5 on WMI decline chronic mechanism.Bilateral carotid artery stenosis (BCAS) model was constructed mimic 7-10 weeks old male mice. Astrocytic Cxcl5 conditional knockout (cKO) mice were with overexpressing generated...
Multimorbidity is common among the middle-aged and elderly residents. And it associated to reduction of health-related quality life (HRQoL), including physical psychological dimensions. However, there are few studies that have paid attention HRQoL residents with multimorbidity in China. Therefore, this study aims investigate relationships between different patterns adults China.Based on a cross-sectional survey, information regarding 18,137 adults, who were at least 45 years age, was...
Alzheimer's disease (AD) is the most common neurodegenerative disorder leading to dementia in elderly, and mechanisms of AD are not fully defined. MicroRNAs (miRNAs) have been shown contribute memory deficits AD. In this study, we identified that miR-204-3p was downregulated hippocampus plasma 6-month-old APPswe/PS1dE9 (APP/PS1) mice. overexpression attenuated synaptic APP/PS1 The amyloid levels oxidative stress were decreased mice after overexpression. Nicotinamide adenine dinucleotide...
Ischemic stroke is a severe and acute neurological disorder with limited therapeutic strategies currently available. Oxidative stress one of the critical pathological factors in ischemia/reperfusion injury, high levels reactive oxygen species (ROS) may drive neuronal apoptosis. Rescuing neurons penumbra potential way to recover from ischemic stroke. Endogenous potent ROS quencher glutathione (GSH) decrease significantly after cerebral ischemia. Here, we aimed investigate neuroprotective...
Dysregulated long non-coding RNAs (lncRNAs) have been shown to contribute the pathogenesis of ischemic stroke. However, potential role lncRNAs in post-stroke microglial activation remains largely unknown. Here, we uncovered that lncRNA-U90926 was significantly increased microglia exposed ischemia/reperfusion both vivo and vitro. In addition, adenovirus-associated virus (AAV)-mediated U90926 silencing alleviated neurological deficits reduced infarct volume experimental stroke mice. Microglial...
Abstract White matter injury (WMI), which reflects myelin loss, contributes to cognitive decline or dementia caused by cerebral vascular diseases. However, because pharmacological agents specifically for WMI are lacking, novel therapeutic strategies need be explored. It is recently found that adaptive myelination required homeostatic control of brain functions. In this study, myelination‐related applied explore the treatment ischemic WMI‐related dysfunction. Here, bilateral carotid artery...
Peripheral neutrophil infiltration can exacerbate ischemia-reperfusion injury. We focused on the relationship between various peripheral immune cells and cerebral (I/R) injury.In this study, we investigated effects of dauricine neuronal injury induced by after ischemic stroke in mouse model, explored undefined mechanisms regulating through RNA sequencing biochemical verification vitro vivo.We found that improved neurological deficits I/R injury, reduced infarct volume, scores. Furthermore,...
Overactivated glial cells, especially microglia, are core components in the progression of pathologic neuroinflammation, and application anti-inflammatory reagents has been regarded as a potential therapy management infarction/reperfusion (I/R) brain injury. This research aims to clarify efect novel lipophilic compound N-(2-[4-tert-butylphenyl]-2-[pyrrolidine-1-yl]ethyl)-7-methyl-4-oxo-4H-chromene-2-carboxamide (named CP-07 this study) LPS-stimulated BV2 cell line primary mouse its...
Due to various factors, there is still a lack of effective neuroprotective agents for ischemic stroke in clinical practice. Neuroinflammation and neuronal apoptosis mediated by endoplasmic reticulum stress are some the important pathological mechanisms stroke. Linarin has been reported have anti-inflammation, antioxidant, anti-apoptotic effects myocardial ischemia, osteoarthritis, kidney disease. Whether it exerts functions not investigated. The results showed that linarin could reduce...
Abstract Background Adenosine A3 receptor (ADORA3) belongs to the adenosine families and role of ADORA3 in vascular dementia (VaD) is largely unexplored. The present study sought determine therapeutic antagonist a mouse model VaD. Methods GSE122063 dataset was selected screen differential expression genes pathways between VaD patients controls. A bilateral carotid artery stenosis (BCAS) established. cognitive functions were examined by novel object recognition test, Y maze fear conditioning...
The inflammatory response plays a pivotal role in blood-brain barrier (BBB) destruction following ischemic brain injury. Enhanced leukocyte adhesion to vascular endothelial cells is an essential event the process. TMEM16A, newly discovered protein regulating calcium-activated chloride channels, widely expressed eukaryotes. Recent studies have suggested that upregulated expression of TMEM16A associated with occurrence and development many diseases. However, BBB integrity after stroke has not...
Abstract Lipid droplets (LDs) were reported to play an important role in the modulation of inflammation and various cellular processes among multiple cell types. However, LDs accumulation, its function mechanisms formation during ischemic stroke remained poorly-identified. In this study, we observed increased accumulation microglia at acute stage by immunofluorescence flow cytometry. Transcriptomic analysis indicated that accumulated with associated phagocytosis. Both inflammatory activation...
To explore the neuroprotective effect of Eugenol Acetate (EA) on post-stroke neuroinflammation and investigate underlying mechanisms. For in vitro experiments, primary microglia were pre-incubated with EA for 2 hours, followed by lipopolysaccharide (LPS) stimulation 24 hours or Oxygen-Glucose Deprivation (OGD) treatment 4 hours. Real-time quantitative PCR, enzyme-linked immunosorbent assay (ELISA) Western blot performed to examine expression levels inflammatory cytokines microglia. The...
Abstract Spatially resolved transcriptomics (SRT) has emerged as a transformative technology for elucidating cellular organization and tissue architecture. However, significant challenge remains in identifying pathology‐relevant spatial functional landscapes within the microenvironment, primarily due to limited integration of cell–cell communication dynamics. To address this limitation, SpaDCN, Spa tially D ynamic graph C onvolutional N etwork framework is proposed, which aligns...
Abstract In the production of aluminum castings, grain refiners are extensively used to achieve desired refinement. However, high cost associated with these materials significantly increases overall expenses casting process. As a result, one primary goals in foundry operations is implement more cost-effective methods This study focuses on innovative use low-energy pulsed electromagnetic energy as an alternative approach for refinement during 5356 alloy. The research conclusively demonstrates...