A5011894444- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Cancer Immunotherapy and Biomarkers
- Trace Elements in Health
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Diagnosis and treatment of tuberculosis
- Immune Response and Inflammation
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Endoplasmic Reticulum Stress and Disease
- Immune cells in cancer
- Cancer Cells and Metastasis
- Prion Diseases and Protein Misfolding
- Immune responses and vaccinations
- Immunodeficiency and Autoimmune Disorders
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer Genomics and Diagnostics
- Bacterial Genetics and Biotechnology
University of Padua
2004-2019
University of Trento
2017
École Polytechnique Fédérale de Lausanne
2010
Mycobacteria lack several of the components that are essential in model systems as Escherichia coli or Bacillus subtilis for formation divisome, a ring-like structure assembling at division site to initiate bacterial cytokinesis. Divisome assembly depends on correct placement FtsZ protein into called Z ring. Notably, early proteins assist localisation ring cytoplasmic membrane and modulate its missing so far known mycobacterial cell machinery. To find mycobacterium-relevant divisome might...
Tightly regulated gene expression systems represent invaluable tools for studying function and the validation of drug targets in bacteria. While several bacterial promoters have been characterized, few them successfully used mycobacteria. In this article we describe development a novel repressible promoter system effective both fast- slow-growing mycobacteria based on two chromosomally encoded repressors, dependent tetracycline (TetR) pristinamycin (Pip), respectively. This uniqueness...
ABSTRACT The proteins belonging to the WhiB superfamily are small global transcriptional regulators typical of actinomycetes. In this paper, we characterize role WhiB5, a Mycobacterium tuberculosis protein superfamily. A null mutant was constructed in M. H37Rv and shown be attenuated during both progressive chronic mouse infections. Mice infected with had smaller bacillary burdens lungs but larger inflammatory response, suggesting WhiB5 immunomodulation. Most interestingly, whiB5 not able...
The Saccharomyces cerevisiae piD261/Bud32 protein and its structural homologues, which are present along the Archaea-Eukarya lineage, constitute a novel kinase family (the piD261 family) distantly related in sequence to eukaryotic superfamily. It has been demonstrated that yeast displays Ser/Thr phosphotransferase activity vitro contains all invariant residues of family. This appears play an important cellular role as deletion gene encoding results alteration fundamental processes such cell...
SigE represents one of the best characterized alternative sigma factors Mycobacterium tuberculosis, playing a major role in response to several environmental stresses and essential for growth macrophages virulence. In previous work we demonstrated that mutant M. tuberculosis which sigE gene was disrupted by cassette conferring hygromycin resistance is promising vaccine candidate better protection than bovis BCG mouse model infection. this describe construction new unmarked entire order...
Pseudomonas aeruginosa pathogenicity island 1 (PAPI-1) is one of the largest genomic islands this important opportunistic human pathogen. Previous studies have shown that PAPI-1 encodes several putative virulence factors, including a major regulator biofilm formation and antibiotic-resistance traits. horizontally transferable into recipient strains lacking via conjugation mediated by specialized type IV pilus. The cluster ten genes associated with synthesis assembly acquisition mechanism...
Despite the great increase in understanding of biology and pathogenesis Mycobacterium tuberculosis achieved by scientific community recent decades, (TB) still represents one major threats to global human health.
Abstract Introduction Targeting immune checkpoint has demonstrated contrasting clinical response in non-small cell lung cancer (NSCLC) due to the lack of robust biomarkers for patients stratification. This is partially a poor understanding role different cellular players (tumor, stromal, infiltrating cells) within tumor microenvironment. Here we present an innovative workflow using DEPArray™ sorting system, isolate classes epithelial and hematopoietic viable single cells from fresh tissue...