A5056274442- Inflammatory Bowel Disease
- Gut microbiota and health
- Microscopic Colitis
- Clostridium difficile and Clostridium perfringens research
- Immunodeficiency and Autoimmune Disorders
- Biosimilars and Bioanalytical Methods
- Eosinophilic Esophagitis
- Diet and metabolism studies
- Fungal and yeast genetics research
- Clinical Nutrition and Gastroenterology
- Gastroesophageal reflux and treatments
- Esophageal Cancer Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Pharmacological Effects of Natural Compounds
- Systemic Lupus Erythematosus Research
- Gastrointestinal motility and disorders
- Enzyme Structure and Function
- Colorectal Cancer Screening and Detection
- Medical and Biological Ozone Research
- Digestive system and related health
- Protein Structure and Dynamics
- Autoimmune and Inflammatory Disorders Research
- Tuberculosis Research and Epidemiology
- Chronic Kidney Disease and Diabetes
University of Padua
2013-2024
Azienda Ospedaliera di Padova
2019
Universidade Federal de Minas Gerais
2010
University of Tübingen
1999-2003
Veneto Institute of Molecular Medicine
2002
TH Bingen University of Applied Sciences
1999
Abstract Background Butyrate has shown anti‐inflammatory and regenerative properties, providing symptomatic relief when orally supplemented in patients suffering from various colonic diseases. We investigated the effect of a colonic‐delivery formulation butyrate on fecal microbiota with inflammatory bowel diseases (IBDs). Methods In this double‐blind, placebo‐controlled, pilot study, 49 IBD (n = 19 Crohn's disease, CD n 30 ulcerative colitis, UC) were randomized to oral administration...
The gastrointestinal tract is home to trillions of diverse microorganisms collectively known as the gut microbiota, which play a pivotal role in breaking down undigested foods, such dietary fibers. Through fermentation these food components, short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced, offering numerous health benefits host. production absorption SCFAs occur through various mechanisms within human intestine, contingent upon types fibers reaching specific...
The gastrointestinal tract is colonized by trillions of different microorganisms, named the gut microbiota, which key to degrade undigested food such as dietary fibers. fermentation these components leads production short-chain fatty acids (SCFA) acetate, propionate, and butyrate, exploit several beneficial roles for host’s health. Their absorption happen in ways human intestine depend on type fiber reaching microorganisms involved fermentation. supplementation SCFAs, mostly...
Ulcerative colitis (UC) is a complex immune-mediated disease in which the gut microbiota plays central role, and may determine prognosis progression. We aimed to assess whether specific profile, as measured by machine learning approach, can be associated with severity patients UC. In this prospective pilot study, consecutive active or inactive UC healthy controls (HCs) were enrolled. Stool samples collected for fecal assessment analysis 16S rRNA gene sequencing approach. A approach was used...
Background/Aim . Inflammatory bowel disease (IBD) is a chronic disorder affecting patients’ quality of life and increasing their disability. The aim our study was to evaluate clinical pharmacological factors associated with impaired disability in large cohort IBD patients during treatment. Methods We consecutively prospectively recruited all referred the Unit “Azienda Ospedaliera” Padua. Demographics information were collected, completed questionnaire (IBDQ) IBD-Disability Index (IBD-DI)...
Abstract. Chronic induction of the prosclerotic cytokine transforming growth factor β (TGF-β) has been implicated in pathogenesis diabetic nephropathy. In a rat model diabetes mellitus-induced glomerulosclerosis, daily administration modified heparin (mH) glycosaminoglycan (GAG) preparation with low anticoagulant activity prevented glomerular and tubular matrix accumulation, as well overexpression TGF-β1 mRNA albuminuria, without obvious side effects. To elucidate molecular mechanisms GAG/mH...
Recent experimental work indicates that the hyperglycemia-induced increase in mesangial matrix production, which is a hallmark development of diabetic nephropathy, mediated by increased expression GLUT1. Mesangial cells stably transfected with human GLUT1 mimic effect hyperglycemia on production extracellular proteins, particularly fibronectin, when cultured under normoglycemic conditions. Our investigation molecular mechanism this has revealed enhanced fibronectin was not prosclerotic...
The Saccharomyces cerevisiae piD261/Bud32 protein and its structural homologues, which are present along the Archaea-Eukarya lineage, constitute a novel kinase family (the piD261 family) distantly related in sequence to eukaryotic superfamily. It has been demonstrated that yeast displays Ser/Thr phosphotransferase activity vitro contains all invariant residues of family. This appears play an important cellular role as deletion gene encoding results alteration fundamental processes such cell...
Epsilon toxin produced by Clostridium perfringens types B and D causes enterotoxemia in sheep, goats calves.Enterotoxemia can cause acute or superacute disease, with sudden death of the affected animal.It provokes huge economic losses when large numbers livestock are affected.Therapeutic intervention is challenging, because disease progresses very rapidly.However, it be prevented immunization specific immunogenic vaccines.We cloned etx gene, encoding epsilon toxin, into vector pET-11a;...
This study describes the formulation optimization and body-cell distribution clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as potential advancement classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely bloodstream; it is slowly captured by filter organs; efficiently cleared within 24-48 h, poorly immunogenic. system displays more favorable properties than its...
To investigate by immunostaining glucose transporter expression in human colorectal mucosa controls and patients with inflammatory bowel disease (IBD).Colorectal samples were obtained from undergoing lower endoscopic colonoscopy or recto-sigmoidoscopy. Patients diagnosed ulcerative colitis (n = 18) Crohn's 10) scheduled for diagnostic enrolled. who underwent prevention screening of cancer followed-up after polypectomy had a history gastrointestinal symptoms designated as the control group...
Anti-drug antibodies can interfere with the activity of anti-tumor necrosis factor (TNF) agents by increasing drug clearance via direct neutralization. The presence anti-drug is clinically relevant when trough concentrations are undetectable or sub-therapeutic. However, traditional immunoassay not easily and rapidly accessible, making translation results into treatment adjustment difficult. availability a point-of-care (POC) test for therapeutic monitoring (TDM) might represent an important...
Patients with triple-negative breast cancer (TNBC) achieving a pathological complete response (pCR) after neoadjuvant chemotherapy have better event-free survival. The role of gut microbiome in early TNBC is underexplored.Microbiome was analyzed by 16SrRNA sequencing.Twenty-five patients treated anthracycline/taxane-based were included. Fifty-six percent achieved pCR. Fecal samples collected before (t0), at 1 (t1), and 8 weeks (t2) from chemotherapy. Overall, 68/75 (90.7%) suitable for...
Inflammatory bowel diseases (IBDs) are treated with anti-TNF agents. Strategies to monitor response therapy may improve clinical control of the disease and reduce economical costs. Previous evidence suggests cleavage infliximab (IFX) by Matrix Metalloproteinase 3 (MMP3) as a mechanism leading loss response. Our study aimed evaluate if MMP3 serum levels could be considered an early marker nonresponse analyze correlation other biochemical markers treatment failure such IFX trough anti-IFX...
Chronic enteropathies (CE) are gastrointestinal diseases that afflict about one in five dogs Europe. Conventional therapeutic approaches include dietary intervention, pharmacological treatment and probiotic supplements. The patient response can be highly variable the interventions often not resolutive. Moreover, strategy is usually planned (and gradually corrected) based on patient’s to empirical treatment, with few indirect gut health indicators useful drive clinicians’ decisions....
Summary Background Data on the role of microbiome in adult patients with eosinophilic oesophagitis (EoE) are limited. Aims To prospectively collect and characterise salivary, oesophageal gastric EoE, further correlating findings disease activity. Methods Adult symptoms dysfunction undergoing upper endoscopy were consecutively enrolled. Patients classified as EoE patients, case more than 15 eosinophils per high‐power field, or non‐EoE controls, lack infiltration. Before during endoscopy,...
Fecal microbiota transplantation (FMT) represents a very promising approach to decreasing disease activity in canine chronic enteropathies (CE). However, the relationship between remission mechanisms and microbiome changes has not been elucidated yet. The main objective of this study was report clinical effects oral freeze-dried FMT CE dogs, comparing fecal microbiomes three groups: pre-FMT CE-affected post-FMT healthy dogs. Diversity analysis, differential abundance machine learning...
The Saccharomyces cerevisiae YGR262c/BUD32 gene, whose disruption causes a severe pleiotropic phenotype, encodes 261-residue putative protein kinase, piD261, structural homologues have been identified in variety of organisms, including humans, and function is unknown. We demonstrated previously that expressed Escherichia coli as recombinant protein, Ser/Thr judged by its ability to autophosphorylate phosphorylate casein. Here we describe mutational analysis showing that, despite low sequence...
Yeast piD261/Bud32 belongs to the piD261 family of atypical protein kinases structurally conserved, from Archaea human. The disruption its gene is causative severely defective growth. Its human homologue, PRPK, interacts with and phosphorylates oncosuppressor p53 protein, which lacking in yeast. Here we show that on one hand vitro, other PRPK can partially complement phenotype yeast encoding piD261/Bud32. These data indicate that, despite considerable structural divergence, members distantly...
The Avidin-Nucleic-Acids-Nano-Assembly (ANANAS) is a kind of soft poly avidin nanoparticle originating from the high affinity interaction between and nucleic acids. In this work we investigated possibility transforming ANANAS cores into stoichiometrically controlled multifunctional nanoparticles through "one-pot" procedure, measured in quantitative way their ability to as reagents for enhanced immunodiagnostic detection. Initially, loading capability biotinylated proteins different nature....
Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed serological analyses. The analysis of pooled samples instead individual ones is a procedure that permits certify, with one single test, entire herds as "disease-free". However, diagnostic need particularly sensitive, especially when levels disease markers are...