A5081682501- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Healthcare and Venom Research
- Nicotinic Acetylcholine Receptors Study
- Marine Invertebrate Physiology and Ecology
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Essential Oils and Antimicrobial Activity
- Insect and Pesticide Research
- Fish Biology and Ecology Studies
- Leech Biology and Applications
- Natural product bioactivities and synthesis
- Phytase and its Applications
- Microbial Metabolites in Food Biotechnology
- Research on Leishmaniasis Studies
- Diphtheria, Corynebacterium, and Tetanus
- Phytochemistry and Biological Activities
- Spider Taxonomy and Behavior Studies
- Antimicrobial Resistance in Staphylococcus
- Fish biology, ecology, and behavior
- Genetic diversity and population structure
Centro Universitário de Sete Lagoas
2016-2020
Universidade Federal de Minas Gerais
2008-2017
Ministry of Education
2015
The Tityus serrulatus scorpion is considered the most dangerous in Brazil and responsible for several cases of human envenomation annually.In this study, we performed transcriptome profiling T. venom gland.In addition to transcripts with housekeeping functions, such as those related protein synthesis, energy supply structural processes, from thirty-five families peptides or proteins were identified.These included three new complete sequences toxins more than a dozen putative gland...
Scorpionism is a public health problem in Brazil, and Tityus serrulatus (Ts) primarily responsible for severe accidents. The main toxic components of Ts venom are low-molecular-weight neurotoxins; however, the also contains poorly characterized high-molecular-weight enzymes. Hyaluronidase one such enzyme that has been characterized.We examined clones from cDNA library gland described two novel isoforms hyaluronidase, TsHyal-1 TsHyal-2. 83% identical, alignment their predicted amino acid...
Wissadula periplocifolia (L.) C. Presl (Malvaceae) is commonly used in Brazil to treat bee stings and as an antiseptic. The antioxidant properties of its extracts have been previously demonstrated, thus justifying a phytochemical investigation for bioactive phenolic constituents. This has yielded five new sulphated flavonoids: 8-O-sulphate isoscutellarein (yannin) (1a); 4′-O-methyl-7-O-sulphate (beltraonin) (1b); 7-O-sulphate acacetin (wissadulin) (2a); 4′-O-methyl-8-O-sulphate (caicoine)...
Tityus serrulatus is a Brazilian scorpion species with great medical significance. While the effects of neurotoxins have been extensively studied, little known about proteases expressed in venom gland this arthropod. In study, clones from T. (Ts) cDNA library were selected according to homology proteases. The sequences aligned database and classified by homology. Similarity identity analyses carried out, phylogenetic tree was constructed other These correspond ten different metalloproteases,...
The hyaluronidase enzyme is generally known as a spreading factor in animal venoms. Although its activity has been demonstrated several organisms, deeper knowledge about and the venom process from bite/sting site until elimination victim's body still need. Herein, we further pursued goal of demonstrating effects inhibition T. serrulatus (TsV) on biodistribution.We used technetium-99m radiolabeled Tityus (99mTc-TsV) to evaluate distribution kinetics mice. To understand hyaluronidase's role...
Envenoming resulting from Loxosceles spider bites (loxoscelism) is a recognized public health problem in Brazil. However, the pathophysiology of loxoscelism caused by L. similis bites, which widespread Brazil, remains poorly understood. In present work, RNA sequencing (RNA-Seq - Next Generation NGS) venom gland was performed to identify and analyze sequences key component phospholipase D. The were aligned based on their classical domains, phylogenetic tree constructed. bioinformatics...
Epsilon toxin produced by Clostridium perfringens types B and D causes enterotoxemia in sheep, goats calves.Enterotoxemia can cause acute or superacute disease, with sudden death of the affected animal.It provokes huge economic losses when large numbers livestock are affected.Therapeutic intervention is challenging, because disease progresses very rapidly.However, it be prevented immunization specific immunogenic vaccines.We cloned etx gene, encoding epsilon toxin, into vector pET-11a;...
Detection of microbial enzymes in natural environments is important to understand biochemical activities and verify the biotechnological potential microorganisms. In present report, 346 isolates from soil, water, plants were screened for enzyme production (caseinase, gelatinase, amylase, carboxymethyl cellulase, esterase). Our results showed that 89.6% produced at least one tested enzyme. A predominance amylase soil samples, cellulase plants, as well esterase gelatinase water was observed....
Scorpion sting envenoming impacts millions of people worldwide, with cardiac effects being one the main causes death on victims. Here we describe first Ca2+ channel toxin present in Tityus serrulatus (Ts) venom, a cell penetrating peptide (CPP) named CPP-Ts. We show that CPP-Ts increases intracellular release through activation nuclear InsP3R cardiomyocytes, thereby causing an increase contraction frequency these cells. Besides proposing novel subfamily active toxins, investigated its...
Members of the spider genus Loxosceles pose a marked health risk to humans because seriousness necrotic and systemic effects their bite, known as loxoscelism. The recent confirmation similis in residences Belo Horizonte Minas Gerais Province, Brazil increases local potential loxoscelism at higher levels. first characterization venom from this species showed that its main biological had similar intensity other (e.g. intermedia, laeta, gaucho). Therefore, we wished further analyse activity L....
Hyaluronidases (HYALs) are enzymes ubiquitously found in venoms from diverse animals and seem to be related venom spreading. HYAL activity might important Tityus spp. envenoming, since anti-Tityus serrulatus (TsHYAL) rabbit antibodies neutralize T. (TsV) lethality. The present work aimed verify compare of other Brazilian (Tityus bahiensis, stigmurus obscurus) test whether anti-TsHYAL horse therapeutic scorpion antivenom (produced by Fundação Ezequiel Dias (FUNED), Butantan Vital Brazil...
Curimat?-pioa (Prochilodus costatus) and curimat?-pacu argenteus) are migratory fish species endemic to the S?o Francisco River Basin in Brazil. Both play important roles local fisheries ecology Paraopeba River. A dam was recently constructed on this river help development conservation programs, we characterized genetic variation of both before construction. Complex hypervariable repeats micro-satellite used asses for within between five collection sites order detect population...
Members of the spider genus Lasiodora are widely distributed in Brazil, where they commonly known as caranguejeiras. venom is slightly harmful to humans. The bite this causes local pain, edema and erythema. However, sp. may be a source important pharmacological tools. Our research group has described previously that produces bradycardia isolated rat heart. In present work, we sought evaluate vascular effect isolate vasoactive compounds from venom. results showed induced...
Background: The hyaluronidase enzyme is generally known as a spreading factor in animal venoms. Although its activity has been demonstrated several organisms, deeper knowledge about and the venom process from bite/sting site until elimination victim's body still need. Methods principal findings: We used technetium-99m radiolabeled Tityus serrulatus (99mTc-TsV) to evaluate distribution kinetics mice. To understand hyaluronidase’s role venom’s biodistribution, 99mTc-TsV was immunoneutralized...