A5031992531- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Parathyroid Disorders and Treatments
- Kidney Stones and Urolithiasis Treatments
- Renal and related cancers
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Biomedical Research and Pathophysiology
- Tissue Engineering and Regenerative Medicine
- TGF-β signaling in diseases
- Renal and Vascular Pathologies
- Cell Adhesion Molecules Research
- Diet and metabolism studies
- Molecular Biology Techniques and Applications
- Systemic Sclerosis and Related Diseases
- Bone and Dental Protein Studies
- Immunodeficiency and Autoimmune Disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Advanced Glycation End Products research
- Blood disorders and treatments
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Acute Kidney Injury Research
- Oral and gingival health research
- Cellular transport and secretion
University of Padua
2012-2025
TH Bingen University of Applied Sciences
1999
University of Tübingen
1999
Ludwig-Maximilians-Universität München
1996
Parietal Epithelial Cells (PECs) activation and proliferation are common to several distinct forms of glomerulopathies. Due stimuli, PECs can change a progenitor (CD24+ CD133/2+) or pro-sclerotic (CD44+) phenotype. In addition, PECs, which constantly exposed filtered albumin, known be involved in albumin internalization, but how this mechanism occurs is unknown. We hypothesized that transport via receptor-mediated endocytosis overload may affect the state PECs. Conditionally immortalized...
Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There increasing evidence of glomerular protein handling by podocytes, but little known about the mechanism behind this process. In study, we found that human podocytes vitro are committed to internalizing albumin through even after exposure low doses albumin. We show these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which partners tubular machinery. Exposing overload prompted an increase...
Abstract. Chronic induction of the prosclerotic cytokine transforming growth factor β (TGF-β) has been implicated in pathogenesis diabetic nephropathy. In a rat model diabetes mellitus-induced glomerulosclerosis, daily administration modified heparin (mH) glycosaminoglycan (GAG) preparation with low anticoagulant activity prevented glomerular and tubular matrix accumulation, as well overexpression TGF-β1 mRNA albuminuria, without obvious side effects. To elucidate molecular mechanisms GAG/mH...
Summary The origin and fate of renal interstitial myofibroblasts (MFs), the effector cells fibrosis, are still debated. Experimental evidence suggests that MFs derive from tubular epithelial throughout epithelial–mesenchymal transition (EMT) process. Primary human (HUTECs) were cultured for 4 6 days on plastic or type I collagen‐coated plates with 1, 5, 10 50 ng/ml transforming growth factor β1 (TGFβ1). EMT process was monitored by morphology immunophenotyping αSMA, cytokeratin 8–18,...
Dent disease (DD) is a rare X-linked recessive renal tubulopathy characterised by low-molecular-weight proteinuria (LMWP), hypercalciuria, nephrocalcinosis and/or nephrolithiasis. DD caused mutations in both the CLCN5 and OCRL genes. encodes electrogenic chloride/proton exchanger ClC-5 which involved tubular reabsorption of albumin LMW proteins, inositol polyphosphate 5-phosphatase, was initially associated with Lowe syndrome. In approximately 25 % patients, no were detected. The aim our...
Glomerular protein handling mechanisms have received much attention in studies of nephrotic syndrome. Histopathological findings renal biopsies from severely proteinuric patients support the likelihood endocytosis by podocytes. ClC-5 is involved albumin proximal tubule. Aim To investigate whether expressed glomerular compartment and it has a role nephropathies. expression was studied using Real-time PCR manually- laser-microdissected with type 2 diabetes (n 37) IgA nephropathy 10);...
Adjuvants, commonly used in vaccines, may be responsible for inducing autoimmunity and autoimmune diseases, both humans mice. The so-called 'ASIA' (Autoimmune/inflammatory Syndrome Induced by Adjuvants) syndrome has been recently described, which is caused the exposure to a component reproducing effect of adjuvants. aim our study was evaluate injection complete Freund's adjuvant (CFA) NZB/NZWF1 mice, lupus-prone murine model. We injected 10 mice with CFA/PBS PBS, three times, 3 weeks apart,...
Abstract Medullary nephrocalcinosis is a hallmark of medullary sponge kidney ( MSK ). We had the opportunity to study spontaneous calcification process in vitro by utilizing renal cells patient with who was heterozygous for c.‐27 + 18G>A variant GDNF gene encoding glial cell‐derived neurotrophic factor. The were obtained collagenase digestion papillary tissues from and two patients no or nephrocalcinosis. These typed immunocytochemistry, presence mineral deposits studied using von Kossa...
Nephrocalcinosis is a clinicopathological entity characterized by microscopic calcium crystals in the renal parenchyma, within tubular lumen or interstitium. Crystal binding to cells may be cause underlying nephrocalcinosis and nephrolithiasis. Pathological circumstances, such as acute cortical necrosis, induce healthy acquire crystal-binding phenotype. The present study aimed investigate whether human proximal (HK-2 cells) can form phosphate deposits under osteogenic conditions, apoptosis...
Dent disease (DD), an X-linked renal tubulopathy, is mainly caused by loss-of-function mutations in CLCN5 (DD1) and OCRL genes. encodes the ClC-5 antiporter that proximal tubules (PT) participates receptor-mediated endocytosis of low molecular weight proteins. Few studies have analyzed PT expression megalin cubilin receptors DD1 kidney biopsies. About 25% DD cases lack either or genes (DD3), no other been discovered so far. Sanger sequencing was used for gene analysis 158 unrelated males...
Dent disease is a rare X-linked renal tubulopathy due to
Dent disease (DD1) is a rare tubulopathy caused by mutations in the CLCN5 gene. Glomerulosclerosis was recently reported DD1 patients and ClC-5 protein shown to be expressed human podocytes. Nephrin actin cytoskeleton play key role for podocyte functions endocytosis seems crucial slit diaphragm regulation. The aim of this study analyze whether loss podocytes might direct consequence glomerular damage patients. Three kidney biopsies presenting focal global glomerulosclerosis four control were...
Understanding how mesenchymal cells arise from epithelial could have a strong impact in unveiling mechanisms of cell plasticity underlying kidney regeneration and repair. In primary human tubular (HUTEC) under different TGF beta 1 concentrations we had observed epithelial-to-mesenchymal transition (EMT) but not epithelial-myofibroblast transdifferentiation. We hypothesized that the process triggered by TGFbeta be dedifferentiation event. The purpose this study is to comprehensively delineate...
Glycosaminoglycan administration has favourable effects on morphological and functional renal abnormalities in different models. The possibility that exogenous glycosaminoglycans modulate glomerular matrix synthesis was explored both primary SV40-MES13 murine mesangial cell cultures. On types, low-molecular-weight heparin showed dose-dependent inhibition of proliferation increase 35SO2−4 uptake. After 36 h the compartment contained a spectrum 35S-molecules less than 200 kDa; under treatment,...