A5069020227- Renal Diseases and Glomerulopathies
- Kidney Stones and Urolithiasis Treatments
- Inflammatory mediators and NSAID effects
- Chronic Kidney Disease and Diabetes
- Ion Transport and Channel Regulation
- Parathyroid Disorders and Treatments
- Biomedical Research and Pathophysiology
- Peroxisome Proliferator-Activated Receptors
- Ion channel regulation and function
- Fatty Acid Research and Health
- Cellular transport and secretion
- Eicosanoids and Hypertension Pharmacology
- Bone Metabolism and Diseases
- Peptidase Inhibition and Analysis
- Acute Kidney Injury Research
- Legal and Labor Studies
- Osteoarthritis Treatment and Mechanisms
- Family and Matrimonial Law
- Heme Oxygenase-1 and Carbon Monoxide
- Metabolism and Genetic Disorders
- Glycogen Storage Diseases and Myoclonus
- Immunodeficiency and Autoimmune Disorders
- Legal processes and jurisprudence
- Cardiac electrophysiology and arrhythmias
- Blood disorders and treatments
University of Padua
2015-2025
Arcispedale Sant'Anna
2016
Società Italiana di Nefrologia
2002
University of Turku
2002
Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There increasing evidence of glomerular protein handling by podocytes, but little known about the mechanism behind this process. In study, we found that human podocytes vitro are committed to internalizing albumin through even after exposure low doses albumin. We show these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which partners tubular machinery. Exposing overload prompted an increase...
Parietal Epithelial Cells (PECs) activation and proliferation are common to several distinct forms of glomerulopathies. Due stimuli, PECs can change a progenitor (CD24+ CD133/2+) or pro-sclerotic (CD44+) phenotype. In addition, PECs, which constantly exposed filtered albumin, known be involved in albumin internalization, but how this mechanism occurs is unknown. We hypothesized that transport via receptor-mediated endocytosis overload may affect the state PECs. Conditionally immortalized...
Epidemiological, clinical and experimental evidence suggests that fatty acids have a modulatory effect on bone metabolism in animals humans. To investigate this hypothesis, we evaluated the effects of three different acids, arachidonic acid (AA), eicosapentaenoic (EPA) oleic (OA), expression cytokines involved remodelling. Cytokine mRNAs human osteoblast-like cell line MG-63 were quantified by reverse transcription-PCR. AA induced increased interleukin-1α, interleukin-1β, tumour necrosis...
There are several pieces of evidence supporting the important role that essential fatty acids (EFAs) and their metabolites play in regulating calcium bone metabolism, relevance to pathobiology disease, with particular reference modulating effects on cytokines. We found arachidonic acid (AA) triggers a cell signal osteoblasts leads expression IL-6. To explore biochemical pathways involved AA induction cytokine gene expression, we evaluated potential protein kinase C (PKC) dependent mechanism...
Abstract Medullary nephrocalcinosis is a hallmark of medullary sponge kidney ( MSK ). We had the opportunity to study spontaneous calcification process in vitro by utilizing renal cells patient with who was heterozygous for c.‐27 + 18G>A variant GDNF gene encoding glial cell‐derived neurotrophic factor. The were obtained collagenase digestion papillary tissues from and two patients no or nephrocalcinosis. These typed immunocytochemistry, presence mineral deposits studied using von Kossa...
Nephrocalcinosis is a clinicopathological entity characterized by microscopic calcium crystals in the renal parenchyma, within tubular lumen or interstitium. Crystal binding to cells may be cause underlying nephrocalcinosis and nephrolithiasis. Pathological circumstances, such as acute cortical necrosis, induce healthy acquire crystal-binding phenotype. The present study aimed investigate whether human proximal (HK-2 cells) can form phosphate deposits under osteogenic conditions, apoptosis...
Dent disease (DD), an X-linked renal tubulopathy, is mainly caused by loss-of-function mutations in CLCN5 (DD1) and OCRL genes. encodes the ClC-5 antiporter that proximal tubules (PT) participates receptor-mediated endocytosis of low molecular weight proteins. Few studies have analyzed PT expression megalin cubilin receptors DD1 kidney biopsies. About 25% DD cases lack either or genes (DD3), no other been discovered so far. Sanger sequencing was used for gene analysis 158 unrelated males...
To investigate the possible role for unsaturated free fatty acids in osteoblast adhesion, effects of two polyunsaturated (PUFAs), arachidonic (AA) and eicosapentaenoic (EPA) acids, one monounsaturated acid, oleic acid (OA), on adhesion to substrate gene expression three extracellular matrix macromolecules were investigated an vitro model system—cultured osteoblast-like human cells. AA, but neither EPA nor OA, diminished bone cell whereas both not increased type I collagen fibronectin via a...
Dent disease is a rare X-linked renal tubulopathy due to
Dent disease (DD1) is a rare tubulopathy caused by mutations in the CLCN5 gene. Glomerulosclerosis was recently reported DD1 patients and ClC-5 protein shown to be expressed human podocytes. Nephrin actin cytoskeleton play key role for podocyte functions endocytosis seems crucial slit diaphragm regulation. The aim of this study analyze whether loss podocytes might direct consequence glomerular damage patients. Three kidney biopsies presenting focal global glomerulosclerosis four control were...
A specific modulatory effect of PUFAs (polyunsaturated fatty acids) on gene expression some cytokines involved in bone remodelling has been reported previously. In particular, although a direct action AA (arachidonic acid) cytokine shown human osteoblastic cells, OA (oleic and EPA (eicosapentaenoic were ineffective. Since the NO (nitric oxide) system also to have an important activity osteoblasts, osteoclasts metabolism, present study we investigated effects iNOS (inducible synthase)...
Abstract The proteoglycan decorin inhibits TGF‐β; therefore, it could antagonize progression of fibrotic diseases associated with activation TGF‐β 1 . effect transfection in human mesangial cells (HMCs) on the expression genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli healthy portions kidneys removed due carcinoma, were histochemically typed. Decorin cDNA cloned a eukaryotic vector transfected into HMCs. Gene fibrogenetic cytokines and proteins , PDGF‐β, α...
To investigate the relationship between polyunsaturated fatty acid (PUFA) and bone metabolism in renal transplant patients, plasma phospholipid (PP) PUFA levels, biochemical markers of turnover mineral density (BMD) were determined 22 recipients a first allograft at baseline after mean 24.4 month follow-up. A significant increase PP n-3 content, [n-3 PUFA/ arachidonic acid] ratio BMD values was observed, as well close correlation content femoral neck BMD. Multivariate regression analysis...
Nephrocalcinosis involves the deposition of microscopic crystals in tubular lumen or interstitium. While clinical, biochemical, and genetic aspects diseases causing nephrocalcinosis have been elucidated, little is known about cellular events this calcification process. We previously reported a phenomenon involving spontaneous formation Ca
An anomalous plasma phospholipid polyunsaturated fatty acid composition has been reported in calcium nephrolithiasis, and was proposed to play a crucial role the pathogenesis of hypercalciuria hyperoxaluria, well-known risk factors for lithogenesis.To confirm this hypothesis, we administered rats three different diets rich coconut, soybean fish oils, evaluated their effect on urinary oxalate excretion, since quality acids represents an important factor able influence activity...