A5056103535- Leptospirosis research and findings
- Gut microbiota and health
- Immunodeficiency and Autoimmune Disorders
- Diet and metabolism studies
- Veterinary Oncology Research
- Veterinary medicine and infectious diseases
- Pancreatic and Hepatic Oncology Research
- Muscle metabolism and nutrition
- Genomics and Phylogenetic Studies
- Pancreatitis Pathology and Treatment
- Microbial Community Ecology and Physiology
- Bioinformatics and Genomic Networks
- Ion channel regulation and function
- Probiotics and Fermented Foods
- Genomics and Rare Diseases
- Plant Genetic and Mutation Studies
- interferon and immune responses
- Peptidase Inhibition and Analysis
- Extracellular vesicles in disease
- Drug-Induced Hepatotoxicity and Protection
- Gene expression and cancer classification
- Genomic variations and chromosomal abnormalities
- Cellular transport and secretion
- Ion Transport and Channel Regulation
- Clostridium difficile and Clostridium perfringens research
Biomolecular Research Genomics (Italy)
2020-2024
University of Padua
2016-2022
Abstract Context We compared the efficacy, safety, and effect of 45-day isocaloric very-low-calorie ketogenic diets (VLCKDs) incorporating whey, vegetable, or animal protein on microbiota in patients with obesity insulin resistance to test hypothesis that source may modulate response VLCKD interventions. Subjects Methods Forty-eight (19 males 29 females, homeostatic model assessment (HOMA) index ≥ 2.5, aged 56.2 ± 6.1 years, body mass [BMI] 35.9 4.1 kg/m2) were randomly assigned three...
Lysosomal storage disorders (LSDs) are monogenic diseases, due to accumulation of specific undegraded substrates into lysosomes. LSD diagnosis could take several years because both poor knowledge these diseases and shared clinical features. The diagnostic approach includes evaluations, biochemical tests, genetic analysis the suspected gene. In this study, we evaluated an targeted sequencing panel as a tool capable potentially reverse classic route. 50 genes 230 intronic sequences conserved...
Cancer is considered a high-risk condition for severe illness resulting from COVID-19. The interaction between acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and human metabolism key to elucidating the risk posed by COVID-19 cancer patients identifying effective treatments, yet it largely uncharacterised on mechanistic level. We present genome-scale map of short-term metabolic alterations triggered SARS-CoV-2 infection cells. Through transcriptomic- proteomic-informed modelling, we...
Abstract OBJECTIVE To evaluate the fecal bacterial microbiota at time of diagnosis (T0) and after 1 month therapy (T1) in cats diagnosed with lymphoplasmacytic enteritis (LPE) or low-grade intestinal T-cell lymphoma (LGITL) to compare these findings those healthy cats. ANIMALS 5 cats, 13 LPE, 7 LGITL were prospectively enrolled between June 2020 2021. METHODS Fecal samples collected T0 T1, DNA was extracted for 16S ribosomal amplicon sequencing. Alpha diversity beta computed. The taxonomic...
The early identification of a subclinical rejection (SCR) can improve the long-term outcome transplanted kidney through intensified immunosuppression. However, only approved diagnostic method is protocol biopsy, which remains an invasive and not without minor and/or major complications. biopsy defined as sampling allograft tissue at pre-established times even in absence impaired renal function; however, it does avoid histological damage. Therefore, discovery new possible biomarkers useful...
Whole genome and exome sequencing are contributing to the extraordinary progress in study of human genetic variants. In this fast developing field, appropriate easily accessible tools required facilitate data analysis.Here we describe QueryOR, a web platform suitable for searching among known candidate genes as well finding novel gene-disease associations. QueryOR combines several innovative features that make it comprehensive, flexible easy use. Instead being designed on specific datasets,...
Dent disease (DD), an X-linked renal tubulopathy, is mainly caused by loss-of-function mutations in CLCN5 (DD1) and OCRL genes. encodes the ClC-5 antiporter that proximal tubules (PT) participates receptor-mediated endocytosis of low molecular weight proteins. Few studies have analyzed PT expression megalin cubilin receptors DD1 kidney biopsies. About 25% DD cases lack either or genes (DD3), no other been discovered so far. Sanger sequencing was used for gene analysis 158 unrelated males...
Antibiotic-responsive enteropathy (ARE) is diagnosed by excluding other causes of diarrhea and when there a short-term response to administration antibiotics.To characterize the gut microbiota clinical trend dogs with suspected ARE evaluate variation in before (T0), after 30 days (T30) tylosin treatment, discontinuation treatment (T60). A further objective was whether changes are related relapses therapy tapered.Study sample (group A) composed 15 chronic diarrhea, group B healthy dogs. Group...
Recent research suggest that gut microbiome may play a fundamental role in athlete's health and performance. Interestingly, nutrition can affect athletic performance by influencing the composition. Among different dietary patterns, ketogenic diet represents an efficient nutritional approach to get adequate body composition athletes, however, some concerns have been raised about its potential detrimental effect on microbiome. To best of our knowledge, only one study investigated athletes...
In mammalian cells, the catabolic activity of dNTP triphosphohydrolase SAMHD1 sets balance and concentration four dNTPs. Deficiency leads to unequally increased pools marked imbalance. Imbalanced increase mutation frequency in cancer but it is not known if SAMHD1-induced imbalance favors accumulation somatic mutations non-transformed cells. Here, we have investigated how fibroblasts from Aicardi-Goutières Syndrome (AGS) patients with mutated react constitutive pool characterized by a huge...
Abstract Natural whey starters (NWS) are undefined bacterial communities produced daily from of the previous cheese-making round, by application high temperature. As a result, in any dairy plant, NWS continuously evolving, mixtures several strains and/or species lactic acid bacteria, whose composition and performance strongly depend on selective pressure acting during incubation. While is critical to assure consistency process, little known about composition, functional features,...
Saprophytic leptospires are spirochetes enclosed within the non-pathogenic clade of genus Leptospira, which in turn is subdivided into two subclades S1 and S2. To date, microorganisms included these have been isolated from environment various parts world, believed to no known animal reservoirs. After a case Leptospira interrogans serovar Pomona was notified owner farm Sardinia, all animals (11 pigs 3 donkeys) were examined for presence Leptospira. Sera tested resulted positive antibodies...
Canine herpesvirus 1 (CaHV-1) infects dogs, causing neonatal death and ocular, neurological, respiratory, reproductive problems in adults. Although CaHV-1 is widespread canine populations, only four studies have focused on the whole genome. In such context, two strains from both kidney spleen of 20-day-old deceased French Bulldog puppies were recently isolated Sardinia, Italy. The extracted viral DNA underwent whole-genome sequencing using Illumina MiSeq platform. Italian genomes nearly...
Aim of this study was to evaluate, the presence and diversity Leptospira spp. in blood urine samples collected from 175 owned-dogs Sardinia, Italy. After determination leptospiral infection by microscopic agglutination test (MAT), MAT-positive dogs were examined real-time polymerase chain reaction (lipL32 rt-PCR) then isolated culture. In order characterize obtained serovars, positive cultures subjected 16S rRNA secY sequencing, phylogenetic analysis Multilocus Sequence Typing (MLST)....
More than one million cases of leptospirosis occur across the globe annually, resulting in about 59,000 deaths. Dogs are most important reservoirs Leptospira species and play an role transmitting pathogen to humans. Many these infections controlled by routine vaccination that has reduced possible reintroduction leptospiral serovars into human population. However, it is still not clear how a vaccinated dog can become infected with or more contained vaccine formulation thus against which...
Leptospirosis is a neglected zoonosis caused by pathogenic spirochetes classified within the genus Leptospira. Among saprophytic species, Leptospira montravelensis was previously reported only from environmental samples. Here, we report whole-genome sequence and annotation of strain that isolated donkey urine during leptospirosis outbreak in Sardinia, Italy.
Background: Oxidative stress, understood as the alteration of physiological equilibrium between production oxygen and nitrogen free radicals their metabolic neutralization (redox imbalance), is a typical condition several pathologies, including neurodegenerative ones.In Parkinson's disease (PD), oxidative phenomena typically interest dopaminergic neurons mesencephalic substantia nigra. Although it unlikely that redox imbalance represents primary event neurodegeneration, certain participates...
MicroRNAs (miRNAs) are a class of non-coding molecules involved in the regulation variety biological processes. They have been identified and characterized several plant species, but only limited data available for Arundo donax L., one most promising bioenergy crops. Here we identified, first time, A. conserved novel miRNAs together with their targets, through combined analysis high-throughput sequencing small RNAs, transcriptome degradome data. A total 134 miRNAs, belonging to 45 families,...
Abstract Down syndrome is a common genetic disorder caused by partial or complete triplication of chromosome 21. This shows an overall and progressive impairment olfactory function, detected early in adulthood. The neuronal cells are located the nasal mucosa represent first sensory neurons pathway. Herein, we applied swabbing procedure to allow gentle collection epithelial seven individuals with ten euploid controls. aim this research was investigate peripheral gene expression pattern...
64 Background: At present, the clinical management of resected GC is only based on risk stratification according to AJCC staging. This project evaluates molecular factors formalin-fixed, paraffin-embedded (FFEP) specimens primary tumor radically in an omogeneous group patients considered at poor prognosis by nodal involvement (pN3a/b 7 th edition) and included ITACA-S trial. Methods: Matched pairs tumor-normal FFPE collected from 15 were subjected whole-exome sequencing using TruSeq Exome...