A5017479216- Prion Diseases and Protein Misfolding
- Muscle Physiology and Disorders
- Trace Elements in Health
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Alzheimer's disease research and treatments
- Neurological diseases and metabolism
- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Cellular transport and secretion
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Biotin and Related Studies
- Genetic Neurodegenerative Diseases
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Alcoholism and Thiamine Deficiency
- X-ray Diffraction in Crystallography
- Graphene and Nanomaterials Applications
- Caveolin-1 and cellular processes
- Neurobiology and Insect Physiology Research
- Crystallization and Solubility Studies
- Tissue Engineering and Regenerative Medicine
Neuroscience Institute
2013-2023
National Research Council
2004-2023
University of Padua
2004-2020
Institute of Neurological Sciences
2020
Istituto Zooprofilattico Sperimentale delle Venezie
2020
Istituto delle Scienze Neurologiche di Bologna
2020
Instituto di Biofisica
2018
Istituto Nazionale di Fisica Nucleare, Sezione di Padova
2013
Silver(i) and gold(i)–N-heterocyclic carbene (NHC) complexes bearing a fluorescent anthracenyl ligand were examined for cytotoxicity in normal tumor cells. The silver(i) complex exhibits greater cells compared with Notably, cell extracts, this determines more pronounced inhibition of thioredoxin reductase (TrxR), but it is ineffective towards glutathione (GR). Both gold silver lead to oxidation the system, derivative being particularly effective. In addition, dimerization peroxiredoxin 3...
In this study, we have tested the hypothesis that augmented [Ca2+] in subcellular regions or organelles, which are known to play a key role cell survival, is missing link between Ca2+ homeostasis alterations and muscular degeneration associated with dystrophy. To end, different targeted chimeras of Ca2+-sensitive photoprotein aequorin been transiently expressed compartments skeletal myotubes mdx mice, animal model Duchenne Direct measurements sarcoplasmic reticulum, [Ca2+]sr, show higher...
Long non-coding RNAs (lncRNAs) are emerging as important players in the regulation of several aspects cellular biology. For a better comprehension their function, it is fundamental to determine tissue or cell specificity and identify subcellular localization. In fact, activity lncRNAs may vary according compartmentalization. Myofibers smallest complete contractile system skeletal muscle influencing its contraction velocity metabolism. How expressed different myofibers, participate metabolism...
Specifically targeted aequorin chimeras were used for studying the dynamic changes of Ca2+ concentration in different subcellular compartments differentiated skeletal muscle myotubes. For cytosol, mitochondria, and nucleus, previously described chimeric aequorins utilized; sarcoplasmic reticulum (SR), a new chimera (srAEQ) was developed by fusing an mutant with low affinity to resident protein calsequestrin. By using appropriate transfection procedure, expression recombinant proteins...
The presence of aggregated alpha-synuclein molecules is a common denominator in variety neurodegenerative disorders. Here, we show that (alpha-syn) an outstanding substrate for the protein tyrosine kinase p72syk (Syk), which phosphorylates three tyrosyl residues its C-terminal domain (Y-125, Y-133, and Y-136), as revealed from experiments with mutants where these have been individually or multiply replaced by phenylalanine. In contrast, only Y-125 phosphorylated Lyn c-Fgr. Eosin-induced...
Abstract Mitochondrial tyrosine phosphorylation is emerging as an important mechanism in regulating mitochondrial function. This article, aimed at identifying which kinases are the major agents phosphorylation, shows that this role should be attributed to Src family members. Indeed, various members of family, for example, Fgr, Fyn, Lyn, c‐Src, constitutively present internal structure mitochondria well Csk, a key enzyme regulation activity family. By means different approaches, biochemical...
It is now well established that the conversion of cellular prion protein, PrP(C), into its anomalous conformer, PrP(Sc), central to onset disease. However, both mechanism prion-related neurodegeneration and physiologic role PrP(C) are still unknown. The use animal cell models has suggested a number putative functions for including signaling, adhesion, proliferation, differentiation. Given skeletal muscles express significant amounts have been related pathophysiology, in present study, we...
Phospholipases A2 are a major component of snake venoms. Some them cause severe muscle necrosis through an unknown mechanism. Phospholipid hydrolysis is possible explanation their toxic action, but catalytic and properties PLA2s not directly connected. In addition, viperid venoms contain PLA2-like proteins, which very even if they lack activity due to critical mutation in position 49. this work, the Bothrops asper myotoxin-II, conjugated with fluorophore TAMRA, was found be internalized...
J. Neurochem. (2011) 116 , 881–890. Abstract The cellular prion protein (PrP C ) is a cell‐surface glycoprotein mainly expressed in the CNS. structural conversion of PrP generates prion, infectious agent causing transmissible spongiform encephalopathies, which are rare and fatal diseases affecting animals humans. Despite decades intensive research, mechanism prion‐associated neurodegeneration physiologic role still obscure. Recent evidence, however, supports hypothesis that may be involved...
Chronic myeloid leukaemia (CML) is driven by the fusion protein Bcr‐Abl, a constitutively active tyrosine kinase playing crucial role in initiation and maintenance of CML phenotype. Despite great efficacy Bcr‐Abl‐specific inhibitor imatinib, resistance to this drug recognized as major problem treatment. We found that LAMA84 cells, characterized imatinib‐resistance caused BCR‐ABL1 gene amplification, pro‐survival CK2 up‐regulated compared sensitive cells. exhibits higher protein‐level...
The prion protein (PrP(C)) is a cell surface glycoprotein mainly expressed in neurons, whose misfolded isoforms generate the responsible for incurable neurodegenerative disorders. Whereas PrP(C) involvement propagation well established, physiological function still enigmatic despite suggestions that it could act signal transduction by modulating phosphorylation cascades and Ca(2+) homeostasis. Because binds neurotoxic aggregates with high-affinity, has also been proposed acts as receptor...
TDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication neurodegenerative disorders. cytosolic inclusions are common neuropathologic hallmark amyotrophic lateral sclerosis (ALS) and related diseases, it now established that misfolding aggregation play key role their etiopathology. neurotoxic mechanisms not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may be promising therapeutic targets proteinopathies....
Direct monitoring of the free Ca2+ concentration in sarcoplasmic reticulum (SR) was carried out rat skeletal myotubes transfected with a specifically targeted aequorin chimera (srAEQ). Myotubes were also chimeric (erAEQ) that we have demonstrated previously is retained endoplasmic (ER). Immunolocalization analysis showed although both recombinant proteins are distributed an endomembrane network identifiable immature SR, erAEQ protein perinuclear membrane. The difficulty measuring [Ca2+]...
AimsThe cellular prion protein, PrPC, whose aberrant isoforms are related to diseases of humans and animals, has a still obscure physiological function. Having observed an increased expression PrPC in two vivo paradigms heart remodelling, we focused on isolated mouse hearts ascertain the capacity antagonize oxidative damage induced by ischaemic non-ischaemic protocols.
Thrombin stimulation of platelets triggers Tyr phosphorylation several signaling proteins, most which remain unidentified. In this study, we demonstrate for the first time that hematopoietic lineage cell-specific protein 1 (HS1) undergoes a transient in human stimulated with thrombin. The is synergistically phosphorylated by Syk and Lyn tyrosine kinases according to sequential mechanism. By means specific inhibitors (PP2, SU6656, piceatannol) phosphopeptide-specific antibodies, as well...