A5074445923- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Cancer Research and Treatments
- RNA Research and Splicing
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Metabolism and Genetic Disorders
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Nuclear Receptors and Signaling
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glioma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
University of Padua
2016-2022
Veneto Institute of Molecular Medicine
2016-2022
Dulbecco Telethon Institute
2016-2017
Medical Research Council
2011-2013
University of Leicester
2011-2013
Institut thématique Génétique, génomique et bioinformatique
2012
Vlaams Instituut voor Biotechnologie
2012
Ghent University Hospital
2012
Ghent University
2012
Innsbruck Medical University
2008-2011
The discovery of the multiple roles mitochondria-endoplasmic reticulum (ER) juxtaposition in cell biology often relied upon exploitation Mitofusin (Mfn) 2 as an ER-mitochondria tether. However, this established Mfn2 function was recently questioned, calling for a critical re-evaluation Mfn2's role cross-talk. Electron microscopy and fluorescence-based probes organelle proximity confirmed that reduced by constitutive or acute deletion. Functionally, mitochondrial uptake Ca2+ released from ER...
p63 inhibits metastasis. Here, we show that (both TAp63 and ΔNp63 isoforms) regulates expression of miR-205 in prostate cancer (PCa) cells, is essential for the inhibitory effects on markers epithelial-mesenchymal transition (EMT), such as ZEB1 vimentin. Correspondingly, effect EMT cell migration reverted by anti-miR-205. p53 mutants inhibit both miR-205, migration, a line expressing endogenous mutated p53, can be abrogated pre-miR-205 or silencing p53. In accordance with this vitro data,...
p73 is a tumor suppressor belonging to the p53 family of transcription factors. Distinct isoforms are transcribed from locus. The use 2 promoters at N-terminus allows expression an isoform containing (TAp73) or not (ΔNp73) complete N-terminal transactivation domain, with latter capable dominant negative effect over former. In addition, both variants alternatively spliced C-terminus. TAp73 bona fide suppressor, being able induce cell death and cycle arrest; conversely, ΔNp73 shows oncogenic...
In autosomal dominant optic atrophy (ADOA), caused by mutations in the mitochondrial cristae biogenesis and fusion protein 1 (Opa1), retinal ganglion cell (RGC) dysfunction visual loss occur unknown mechanisms. Here, we show a role for autophagy ADOA pathogenesis. RGCs expressing mutated Opa1, active 5' AMP-activated kinase (AMPK) its effector ULK1 accumulate at axonal hillocks. This AMPK activation triggers localized hillock autophagosome accumulation mitophagy, ultimately resulting reduced...
Mitochondrial fusion and fission proteins have been nominated as druggable targets in cancer. Whether their inhibition is efficacious triple negative breast cancer (TNBC) that almost invariably develops chemoresistance unknown.
Long non-coding RNAs (lncRNAs) are emerging as important players in the regulation of several aspects cellular biology. For a better comprehension their function, it is fundamental to determine tissue or cell specificity and identify subcellular localization. In fact, activity lncRNAs may vary according compartmentalization. Myofibers smallest complete contractile system skeletal muscle influencing its contraction velocity metabolism. How expressed different myofibers, participate metabolism...
Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference regulated in these post-mitotic cells largely unknown, making this issue a key question the fields and whole-body metabolism. Here, we show microRNAs (miRNAs) play role defining metabolic profiles. mRNA miRNA signatures all obtained at single-cell level unveiled fiber-specific regulatory networks identified two master miRNAs coordinately control...
p63 is a p53 family transcription factor, which besides unique roles in epithelial development, shares tumor suppressive activity with its homolog p53. The gene has different transcriptional start sites, generate two N-terminal isoforms (transactivation domain (TA)p63 and amino terminal truncated protein(ΔN)p63); addition alternative splicing at the 5′-end give rise to least five C-terminal isoforms. This complexity of structure probably fostered debate controversy on function cancer,...
The transcription factor p73 belongs to the p53 family of tumour suppressors and similar other members, transcribed as different isoforms with opposing pro- anti-apoptotic functions. Unlike p53, mutations are extremely rare in cancers. Instead, pro-apoptotic activities transcriptionally active commonly inhibited by over-expression dominant negative isoforms. Therefore relative ratio is critical for cellular response a chemotherapeutic agent. Here, we analysed expression N-terminal cell lines...
p73 is a p53 family transcription factor. Due to the presence in 5' flanking region of two promoters, there are N-terminal variants, TAp73, which retains fully active transactivation domain (TA), and ΔNp73, N terminus truncated. In addition, extensive 3' splicing gives rise at least seven distinctive isoforms; TAp73-selective knockout highlights its role as regulator cell death, senescence tumor suppressor. ΔNp73-selective knockout, on other hand, anti-apoptotic function ΔNp73 involvement...
Abstract The tumour suppressor p53 plays an important role in somatic cell reprogramming. While wild-type reduces reprogramming efficiency, mutant exerts a gain of function activity that leads to increased efficiency. Furthermore, induced pluripotent stem cells expressing lose their pluripotency vivo and form malignant tumours when injected mice. It is therefore great interest identify targets (wild type mutant) are responsible for this phenotype during reprogramming, as these could be...
P73 is a member of the p53 transcription factors family with prominent role in neurobiology, affecting brain development as well controlling neuronal survival. Accordingly, p73 has been identified key player many age-related neurodegenerative diseases, such Alzheimer's disease, neuroAIDS and Niemann-Pick type C disease. Here we investigate possible correlations Parkinson Tyrosine hydroxylase crucial disease being enzyme necessary for dopamine synthesis. In this work show that levels tyrosine...
Different bacteria-derived systems for regulatable gene expression have been developed the use in mammalian cells and some were also successfully adopted vivo vertebrate model organisms. However, certain limitations apply to most of these systems, including leakiness transgene expression, inefficient silencing or activation, as well limited tissue accessibility transgene-inducers their unfavourable pharmacokinetics. In this study, we evaluated suitability lac-operon/lac-repressor (lacO/lacI)...
SUMMARY Skeletal muscle is composed by different myofiber types that can preferentially use glycolysis or lipids for ATP production. How fuel preference specified in these post-mitotic cells unknown. Here we show miRNAs are important players defining the metabolic profile. mRNA and miRNA signatures of all obtained at single cell level unveiled fiber-specific regulatory networks identified two master coordinately control mitochondrial morphology. Our work provides a complete integrated...