A5011184674- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- Neuropeptides and Animal Physiology
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Trace Elements in Health
- Respiratory viral infections research
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
- Influenza Virus Research Studies
- Prion Diseases and Protein Misfolding
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Genetic Neurodegenerative Diseases
- RNA modifications and cancer
- Neurological diseases and metabolism
Université de Sherbrooke
2006-2017
PROTEO
2017
Université Laval
2005
A fully mature mRNA is usually associated to a reference open reading frame encoding single protein. Yet, mRNAs contain unconventional alternative frames (AltORFs) located in untranslated regions (UTRs) or overlapping the ORFs (RefORFs) non-canonical +2 and +3 frames. Although recent ribosome profiling footprinting approaches have suggested significant use of translation initiation sites mammals, direct evidence large-scale protein expression at proteome level still lacking. To determine...
Recent functional, proteomic and ribosome profiling studies in eukaryotes have concurrently demonstrated the translation of alternative open-reading frames (altORFs) addition to annotated protein coding sequences (CDSs). We show that a large number small proteins could fact be coded by these altORFs. The putative translated from altORFs orthologs many species contain functional domains. Evolutionary analyses indicate often more extreme conservation patterns than their CDSs. Thousands are...
Spinocerebellar ataxia type 1 is an autosomal dominant cerebellar associated with the expansion of a polyglutamine tract within ataxin-1 (ATXN1) protein. Recent studies suggest that understanding normal function ATXN1 in cellular processes essential to decipher pathogenesis mechanisms spinocerebellar 1. We found alternative translation initiation ATG codon +3 reading frame human starting 30 nucleotides downstream for and ending at nucleotide 587. This novel overlapping open (ORF) encodes...
An endoproteolytic cleavage termed α-cleavage between residues 111/112 is a characteristic feature of the cellular prion protein (PrP C ). This generates soluble N-terminal fragment (PrPN1) and glycosylphosphatidylinositol-anchored C-terminal (PrPC1). Independent studies demonstrate that modulating PrP represents potential therapeutic strategy in diseases. The regulation unclear. only known domain essential for to occur hydrophobic (HD). Importantly, HD also formation homodimers. To explore...
Influenza A virus seasonal outbreaks and occasional pandemics represent a global health threat. The high genetic instability of this permits rapid escape from the host immune system emergence resistance to antivirals. There is thus an urgent need develop novel approaches for efficient treatment newly emerging strains. Based on sequence alignment representatives every subtype known infect humans, we identified nucleic acid regions that are conserved amongst these influenza populations. We...
ABSTRACT In this study, the dipeptidyl peptidase IV (DPP IV) of swine pathogen Streptococcus suis was cloned, overexpressed in Escherichia coli , and characterized. The coding region comprises 2,268 nucleotides containing an open reading frame that codes for a 755-amino-acid protein with calculated molecular mass 85 kDa. amino acid sequence contained Gly-X-Ser-X-X-Gly, which is consensus motif flanking active-site serine shared by proteases. recombinant DPP showed high affinity synthetic...
Abstract Recent studies in eukaryotes have demonstrated the translation of alternative open reading frames (altORFs) addition to annotated protein coding sequences (CDSs). We show that a large number small proteins could fact be coded by altORFs. The putative translated from altORFs orthologs many species and evolutionary patterns indicate are particularly constrained CDSs evolve slowly. Thousands predicted detected proteomic datasets reanalysis using database containing proteins. Protein...