A5031390110- Monoclonal and Polyclonal Antibodies Research
- Prostate Cancer Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Cell Image Analysis Techniques
- Organ Transplantation Techniques and Outcomes
- Viral Infections and Immunology Research
- Geriatric Care and Nursing Homes
- Advanced Biosensing Techniques and Applications
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Syphilis Diagnosis and Treatment
- Liver Disease and Transplantation
- Influenza Virus Research Studies
- Genital Health and Disease
- Long-Term Effects of COVID-19
- Advanced biosensing and bioanalysis techniques
- Renal Transplantation Outcomes and Treatments
- COVID-19 and healthcare impacts
- Transplantation: Methods and Outcomes
- 3D Printing in Biomedical Research
- Molecular Biology Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Urologic and reproductive health conditions
- Palliative Care and End-of-Life Issues
- Biosensors and Analytical Detection
Science for Life Laboratory
2021-2025
Uppsala University
2021-2025
The Ohio State University
2023
Massachusetts General Hospital
2022-2023
Harvard University
2022-2023
University of Chicago
2019-2022
University of Illinois Chicago
2019
Background A definitive diagnosis of prostate cancer requires a biopsy to obtain tissue for pathologic analysis, but this is an invasive procedure and associated with complications. Purpose To develop artificial intelligence (AI)‐based model (named AI‐biopsy) the early using magnetic resonance (MR) images labeled histopathology information. Study Type Retrospective. Population Magnetic imaging (MRI) data sets from 400 patients suspected histological (228 acquired in‐house 172 external...
An electrochemical biosensor based on modified carbon screen-printed electrodes was developed for the detection of hemagglutinin influenza A H1N1 virus (H1). Gold nanoflowers were electrodeposited electrode to increase conductivity and surface area. The signal amplified by functionalization gold with 4-aminothiophenol, which resulted in a 100-fold decrease charge transfer resistance due tunneling effect. Subsequently, monoclonal antibodies against H1 immobilized via covalent amide bond...
An electrochemical biosensor based on modified carbon screen-printed electrodes (CSPE) was developed for the detection of hemagglutinin influenza A H1N1 virus (H1). Gold nanoflowers were electrodeposited electrode to increase conductivity and surface area. The signal amplified by functionalization gold with 4-aminothiophenol, which resulted in a 100-fold decrease charge transfer resistance due tunneling effect. Subsequently, monoclonal antibodies against H1 immobilized via covalent amide...
Normothermic machine perfusion (NMP) allows viability assessment and potential resuscitation of donor livers prior to transplantation. The immunological effect NMP on liver allografts is undetermined, with implications allograft function, rejection outcomes overall survival. In this study we define the changes in immune profile human during NMP.Six were placed a device. Tissue perfusate samples obtained cold storage at 1, 3, 6 hours perfusion. Flow cytometry, immunohistochemistry, bead-based...
BackgroundAs the COVID-19 pandemic moves into survivorship phase, questions regarding long-term lung damage remain unanswered. Previous histopathologic studies are limited to autopsy reports. We studied specimens from survivors who underwent elective resections determine whether postacute changes present.MethodsThis multicenter observational study included 11 adult had recovered but subsequently unrelated resection for indeterminate nodules or cancer. compared these against an age- and...
Difficulty achieving complete, specific, and homogenous staining is a major bottleneck preventing the widespread use of tissue clearing techniques to image large volumes human tissue. In this manuscript, we describe procedure rapidly design immunostaining protocols for antibody labeling cleared brain We prepared libraries 0.5-1.0 mm thick sections that are fixed, pre-treated, via similar, but different procedures optimize conditions panel antibodies. Results from library mouse correlate well...
Ex vivo lung perfusion (EVLP) is used to assess and preserve lungs prior transplantation. However, its inherent immunomodulatory effects are not completely understood. We examine perfusate tissue compartments determine the change in immune cell composition human maintained on EVLP.Six unsuitable for transplantation underwent EVLP. Tissue samples were obtained during cold storage at 1-, 3- 6-h perfusion. Flow cytometry, immunohistochemistry, bead-based immunoassays measure leukocyte...
Abstract Magnetic Resonance Imaging (MRI) is routinely used to visualize the prostate gland and manage cancer. The Prostate Reporting And Data System (PI-RADS) evaluate clinical risk associated with a potential tumor. However PI-RADS score subjective its assessment varies between physicians. As result, definite diagnosis of cancer requires biopsy obtain tissue for pathologic analysis. A an invasive procedure complications, including hematospermia, hematuria, rectal bleeding. We hypothesized...
Influenza A virus belongs to the Orthomyxoviridae family and, date, is one of most important pathogens causing acute respiratory infections, such as recent pandemic 2009. Hemagglutinin (HA) surface proteins that allow it interact with cellular molecules. Due fact abundant protein in capsule, best target detection H1N1 through biosensing devices. Our aim develop an electrochemical biosensor detect H1 by modifying carbon screen-printed electrodes (CSPE) gold nanoparticles and add further...
Abstract Difficulty achieving complete, specific, and homogenous staining is a major bottleneck preventing the widespread use of tissue clearing techniques to image large volumes human tissue. In this manuscript, we describe procedure rapidly design immunostaining protocols for antibody labeling cleared brain We prepared libraries .5-1.0 mm thick sections that are fixed, pre-treated, via similar, but different procedures optimize conditions panel antibodies. Results from library mouse...