A5004704133- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Tryptophan and brain disorders
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Molecular Junctions and Nanostructures
- Chronic Lymphocytic Leukemia Research
- Cell Adhesion Molecules Research
- Bacterial Genetics and Biotechnology
- Stress Responses and Cortisol
- Molecular Biology Techniques and Applications
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Lymphoma Diagnosis and Treatment
- Diabetes and associated disorders
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- Click Chemistry and Applications
- Acute Lymphoblastic Leukemia research
- Virology and Viral Diseases
Science for Life Laboratory
2016-2025
Uppsala University
2016-2025
Mount Kenya University
2024
Pediatrics and Genetics
2014-2019
Centre d’études d’agents Pathogènes et Biotechnologies Pour la Santé
2004
University of California, San Diego
2003
Prostasomes are microvesicles (mean diameter, 150 nm) that produced and secreted by normal malignant prostate acinar cells. It has been hypothesized invasive growth of cells may cause these microvesicles, normally released into seminal fluid, to appear in interstitial space therewith peripheral circulation. The suitability prostasomes as blood biomarkers patients with cancer was tested using an expanded variant the proximity ligation assay (PLA). We developed extremely sensitive specific...
Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because the heterogeneity it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay profile surface individual using antibody-DNA conjugates next-generation sequencing. We first validate method artificial...
The neurotransmitter γ-aminobutyric acid (GABA) is an extracellular signaling molecule in the brain and pancreatic islets. Here, we demonstrate that GABA regulates cytokine secretion from human peripheral blood mononuclear cells (PBMCs) CD4+ T cells. In anti-CD3 stimulated PBMCs, (100 nM) inhibited release of 47 cytokines patients with type 1 diabetes (T1D), but only 16 nondiabetic (ND) individuals. ND individuals were grouped into responder or non-responder according to effects nM, 500 on...
Extracellular vesicles (EVs) are membrane-coated objects such as exosomes and microvesicles, released by many cell-types. Their presence in body fluids the variable surface composition content render them attractive potential biomarkers. The ability to determine their cellular origin could greatly move field forward. We used multiplex proximity extension assays (PEA) identify with high specificity sensitivity protein profiles of different origins, including seven cell lines two fluids. By...
Abstract Sensitive detection of protein interactions and post-translational modifications native proteins is a challenge for research diagnostic purposes. A method this, which could be used in point-of-care devices high-throughput screening, should reliable, cost effective robust. To achieve here we design (proxHCR) that combines the need proximal binding with hybridization chain reaction (HCR) signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind close...
An important motivation for the construction of biobanks is to discover biomarkers that identify diseases at early, potentially curable stages. This will require from large numbers individuals, preferably sampled repeatedly, where samples are collected and stored under conditions preserve potential biomarkers. Dried blood attractive biobanking because ease low cost collection storage. Here we have investigated their suitability protein measurements. Ninety-two proteins with relevance...
Detection of proteins released in the bloodstream from tissues damaged by disease can promote early detection pathological conditions, differential diagnostics, and follow-up therapy. Despite these prospects a plethora candidate biomarkers, efforts recent years to establish new protein diagnostic assays have met with limited success. One important limiting factor has been challenge detecting present at trace levels complex bodily fluids. To achieve robust, sensitive, specific detection, we...
Despite intense interest, methods that provide enhanced sensitivity and specificity in parallel measurements of candidate protein biomarkers numerous samples have been lacking. We present herein a multiplex proximity ligation assay with readout via realtime PCR or DNA sequencing (ProteinSeq). demonstrate improved over conventional sandwich assays for simultaneous analysis sets 35 proteins 5 µl blood plasma. Importantly, we observe minimal tendency to increased background multiplexing,...
Gastric cancer (GC) is the third leading cause of death. Early detection a key factor to reduce its mortality.We retrospectively collected pre- and postoperative serum samples as well tumour tissues adjacent normal from 100 GC patients. Serum non-cancerous patients were served controls (n = 50). A high-throughput protein technology, multiplex proximity extension assays (PEA), was applied measure levels over 300 proteins. Alteration each analysed by univariate analysis. Elastic-net logistic...
Recent studies indicate that the immune system adaptation during pregnancy could play a significant role in pathophysiology of perinatal depression. The aim this study was to investigate if inflammation markers late plasma sample can predict presence depressive symptoms at eight weeks postpartum. Blood samples from 291 pregnant women (median and IQR for days delivery, 13 7-23days respectively) comprising 63 individuals with postpartum symptoms, as assessed by Edinburgh postnatal depression...
Abstract Postpartum depression (PPD) is a devastating disorder affecting not only more than 10% of all women giving birth, but also the baby, family, and society. Compiling evidence suggests involvement immune system in pathophysiology major depression; yet, response perinatal as well studied. The aim this study was to investigate alterations peripheral levels inflammatory biomarkers 169 Swedish with without depressive symptoms according Edinburgh postnatal scale or M.I.N.I neuropsychiatric...
Abstract Lubricin, a heavily O -glycosylated protein, is essential for boundary lubrication of articular cartilage. Strong surface adherence lubricin required given the extreme force it must withstand. Disulfide bound complexes and cartilage oligomeric matrix protein (COMP) have recently been identified in arthritic synovial fluid suggesting they may be lost from osteoarthritis inflammatory arthritis. This investigation was undertaken to localise COMP-lubricin within investigate if other...
Significance Here, we show that FilP, a bacterial cytoskeletal protein related to metazoan intermediate filament (IF) proteins, can self-assemble into regular network structure. This finding offers possible explanation for its previously characterized role in cellular rigidity and elasticity might offer insights the mechanical of human IFs. The assembly FilP cytoskeleton is coupled function polarisome, complex orchestrating polar growth characteristic Streptomyces . These results suggest...
Despite great interest, investments, and efforts, the ongoing search for plasma protein biomarkers disease so far has come up surprisingly empty-handed. Although discovery programs have revealed large numbers of biomarker candidates, clinical utility been validated only a very small number these. While this disappointing state affairs may suggest that little more to offer diagnostics, we take perspective experimental conditions might not optimal analyses will be required greater sensitivity...
Flow cytometry is a powerful method for quantitative and qualitative analysis of individual cells. However, flow cytometric extracellular vesicles (EVs), the proteins present on their surfaces has been hampered by small size EVs - in particular smallest EVs, which can be as little 40 nm diameter, limited number antigens present, low refractive index. We addressed these limitations detection characterization EV through use multiplex multicolor situ proximity ligation assays (in PLA), allowing...
Abstract Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory‐based purification techniques rely on the physical properties small‐EVs rather than their inherited fingerprints. We established highly‐selective assay, termed EV‐CATCHER, initially designed for high‐throughput analysis low‐abundance small‐RNA cargos by...
Protein-binding DNA sequence elements encode a variety of regulated functions genomes. Information about such is currently in state rapid growth, but improved methods are required to characterize the specificity DNA-binding proteins. We have established an vitro method for specific and sensitive solution-phase analysis interactions between proteins nucleic acids nuclear extracts, based on proximity ligation assay. The reagent consumption very low, excellent sensitivity assay enables as few...
The appearance of neurofibrillary tangles (NFT), one the major hallmarks Alzheimer's disease (AD), is most likely caused by inappropriate phosphorylation and/or dephosphorylation tau, eventually leading to accumulation NFTs. Enhanced
Abstract Background A number of factors regarding blood collection, handling and storage may affect sample quality. The purpose this study was to assess the impact on plasma protein profiles by delayed centrifugation separation multiple freeze-thaw cycles. Methods Blood samples drawn from 16 healthy individuals were collected into ethylenediaminetetraacetic acid tubes kept either at 4 °C or 22 for 1–36 h prior centrifugation. Plasma prepared 1 after venipuncture also subjected two eight...
Earlier studies suggest that lumbar radicular pain following disc herniation may be associated with a local or systemic inflammatory process. In the present study, we investigated serum protein profile of such patients. All 45 patients were recruited from Oslo University Hospital, Ullevål, Norway, during period 2007–2009. The new multiplex proximity extension assay (PEA) technology was used to analyze levels 92 proteins. Interestingly, data showed 12 months after different other regard many...