A5023502226- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Liver Disease Diagnosis and Treatment
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Advanced Proteomics Techniques and Applications
- Diabetes and associated disorders
- Wnt/β-catenin signaling in development and cancer
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Cancer-related gene regulation
- ATP Synthase and ATPases Research
- Drug Transport and Resistance Mechanisms
- Lymphoma Diagnosis and Treatment
- Salivary Gland Disorders and Functions
- Blood properties and coagulation
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- Pancreatic and Hepatic Oncology Research
- Advanced biosensing and bioanalysis techniques
- Chronic Lymphocytic Leukemia Research
- Cancer, Lipids, and Metabolism
Uppsala University
2016-2025
Science for Life Laboratory
2016-2023
University of North Carolina at Chapel Hill
2022
Institute of Hematology & Blood Diseases Hospital
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
National Clinical Research
2021
Pediatrics and Genetics
2019
Renji Hospital
2012-2016
Shanghai Jiao Tong University
2010-2016
Shanghai Cancer Institute
2012-2016
Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because the heterogeneity it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay profile surface individual using antibody-DNA conjugates next-generation sequencing. We first validate method artificial...
The neurotransmitter γ-aminobutyric acid (GABA) is an extracellular signaling molecule in the brain and pancreatic islets. Here, we demonstrate that GABA regulates cytokine secretion from human peripheral blood mononuclear cells (PBMCs) CD4+ T cells. In anti-CD3 stimulated PBMCs, (100 nM) inhibited release of 47 cytokines patients with type 1 diabetes (T1D), but only 16 nondiabetic (ND) individuals. ND individuals were grouped into responder or non-responder according to effects nM, 500 on...
An important motivation for the construction of biobanks is to discover biomarkers that identify diseases at early, potentially curable stages. This will require from large numbers individuals, preferably sampled repeatedly, where samples are collected and stored under conditions preserve potential biomarkers. Dried blood attractive biobanking because ease low cost collection storage. Here we have investigated their suitability protein measurements. Ninety-two proteins with relevance...
Abstract BACKGROUND: Dickkopf‐related protein 1 (DKK1) has been reported involved in metastasis and invasion several tumors. This study sought to investigate the prognostic value of DKK1 intrahepatic cholangiocarcinoma (ICC) its role promoting ICC metastasis. METHODS: Tissue microarrays 138 patient samples were employed detect DKK1, vascular endothelial growth factor C (VEGF‐C), matrix metalloproteinase 9 (MMP9) expression using immunohistochemistry. The significances assessed by...
Gastric cancer (GC) is the third leading cause of death. Early detection a key factor to reduce its mortality.We retrospectively collected pre- and postoperative serum samples as well tumour tissues adjacent normal from 100 GC patients. Serum non-cancerous patients were served controls (n = 50). A high-throughput protein technology, multiplex proximity extension assays (PEA), was applied measure levels over 300 proteins. Alteration each analysed by univariate analysis. Elastic-net logistic...
Abstract Background A number of factors regarding blood collection, handling and storage may affect sample quality. The purpose this study was to assess the impact on plasma protein profiles by delayed centrifugation separation multiple freeze-thaw cycles. Methods Blood samples drawn from 16 healthy individuals were collected into ethylenediaminetetraacetic acid tubes kept either at 4 °C or 22 for 1–36 h prior centrifugation. Plasma prepared 1 after venipuncture also subjected two eight...
Hypoxic microenvironment is a powerful driving force for the invasion and metastasis of hepatocellular carcinoma (HCC). Hypoxia-inducible factor 1α (HIF-1α), as crucial regulator transcriptional responses to hypoxia, induces expression multiple target genes involved in different steps HCC metastatic process. It critical find associated with under hypoxia shedding new light on molecular mechanism metastasis. In this study, we uncovered that could induce upregulation Rab11-family interacting...
Traumatic brain injury (TBI) is followed by secondary mechanisms strongly involving neuroinflammation. To monitor the complex inflammatory cascade in human TBI, we used cerebral microdialysis (MD) and multiplex proximity extension assay (PEA) technology simultaneously measured levels of 92 protein biomarkers inflammation MD samples every three hours for five days 10 patients with severe TBI under neurointensive care. One μL were incubated paired oligonucleotide-conjugated antibodies binding...
Immunomodulation is increasingly being recognised as a part of mental diseases. Here, we examined whether levels immunological protein markers changed with depression, age, or the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). An analysis plasma samples from patients major depressive episode and control blood donors (CBD) revealed expression 67 inflammatory markers. Thirteen these displayed augmented in compared to CBD. Twenty-one correlated age patients, whereas 10...
Abstract Extracellular vesicles (EVs) are mediators of intercellular communication and a promising class biomarkers. Surface proteins EVs play decisive roles in establishing connection with recipient cells, they putative targets for diagnostic assays. Analysis the surface can thus both illuminate biological functions help identify potential We developed strategy combining high-resolution mass spectrometry (HRMS) proximity ligation assays (PLA) to first then validate discovered on EVs....
Summary In chronic lymphocytic leukaemia ( CLL ) patients, treatment with the Bruton tyrosine kinase inhibitor ibrutinib induces a rapid shift of tumour cells from lymph nodes LN to peripheral blood PB ). Here, we characterized in depth dynamics ibrutinib‐induced inflammatory, transcriptional and cellular changes different compartments immediately after initiation seven relapsed/refractory patients. Serial samples were taken before start during first 29 days treatment. Changes plasma...
Surgery on the arch or descending aorta is associated with significant risk of neurological complications. As a consequence intubation and sedation, early neurologic injury may remain unnoticed. Biomarkers to aid in initial diagnostics could prove great value as immediate intervention critical. Twenty-three patients operated thoracic perioperative were included. Cerebrospinal fluid (CSF) serum obtained preoperatively first second postoperative days assessed panel 92 neurological-related...
Aim: To evaluate prognostic significance of DKK1 for hepatocelluar carcinoma. Materials & methods: We enrolled a test cohort consisting 266 hepatitis virus B-related carcinoma patients who had undergone hepatectomy and validation 95. Associations with overall survival time to recurrence were determined by Cox proportional hazards regression model. Results: High levels preoperative serum associated poor higher rate was an independent predictor. Moreover, maintained ability predict low risk....
We analyse and compare MUC7<italic>O</italic>-glycosylation inflammatory biomarkers in saliva from female patients with burning mouth syndrome (BMS) gender/age-matched controls.