A5073336032- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- FOXO transcription factor regulation
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Mathematical Biology Tumor Growth
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immune cells in cancer
- RNA modifications and cancer
- Cancer Research and Treatments
- vaccines and immunoinformatics approaches
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Microtubule and mitosis dynamics
- Circular RNAs in diseases
Erasmus MC
2020-2022
State Key Laboratory of Oncogene and Related Genes
2014-2017
Renji Hospital
2014-2017
Shanghai Jiao Tong University
2014-2017
Shanghai Cancer Institute
2014-2017
BACKGROUND: It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have reported in hepatocellular carcinoma (HCC).OBJECTIVE: To estimate clinical values of a RNA, Hsa_circ_0001649, HCC.METHODS: Expression level hsa_circ_0001649 was detected HCC and paired adjacent liver tissues by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs).Differences expression were analyzed using the t-test.Tests performed between...
Cancer-associated fibroblasts (CAFs) play a key role in the cancer process, but research progress is hampered by paucity of preclinical models that are essential for mechanistic dissection cell-CAF interactions. Here, we aimed to establish 3-dimensional (3D) organotypic co-cultures primary liver tumor-derived organoids with CAFs, and understand their interactions response treatment.Liver tumor CAFs were cultured from murine human tumors. 3D co-culture Transwell culture systems established...
Background & AimsTIGIT is a co-inhibitory receptor, and its suitability as target for cancer immunotherapy in HCC unknown. PD1 blockade clinically effective about 20% of advanced patients. Here we aim to determine whether co-blockade TIGIT/PD1 has added value restore functionality tumor-infiltrating T cells (TILs).MethodsMononuclear leukocytes were isolated from tumors, paired tumor-free liver tissues (TFL) peripheral blood patients, used flow cytometric phenotyping functional assays....
Abstract The use of cytokines for immunotherapy shows clinical efficacy but is frequently accompanied by severe adverse events caused excessive and systemic immune activation. Here, we set out to address these challenges engineering a fusion protein single, potency-reduced, IL15 mutein PD1-specific antibody (anti-PD1-IL15m). This immunocytokine was designed deliver PD1-mediated, avidity-driven IL2/15 receptor stimulation PD1+ tumor-infiltrating lymphocytes (TIL) while minimally affecting...
Abstract Background Immunotherapy with immune checkpoint inhibitors (ICIs) is being explored to improve cholangiocarcinoma (CCA) therapy. However, it remains difficult predict which ICI will be effective for individual patients. Therefore, the aim of this study develop a co-culture method patient-derived CCA organoids and cells, could represent anti-cancer immunity in vitro. Methods were co-cultured peripheral blood mononuclear cells or T cells. Flow cytometry, time-lapse confocal imaging...
Reprogrammed metabolism has been identified as an emerging hallmark in cancer cells. It demonstrated that fructose-1, 6-bisphosphatase 1 (FBP1) a rate-limiting enzyme gluconeogenesis plays critical roles tumor initiation and progression several types. However, function of FBP1 hepatocellular carcinoma (HCC) is still not clear. In this study, we observed the expression was obviously downregulated cell lines tissues HCC. Downregulation HCC correlated with lower overall survival rate had...
Emerging evidence has indicated that deregulation of long non‐coding RNA s (lnc s) can contribute to the progression and metastasis human cancer, including hepatocellular carcinoma ( HCC ). However, roles most lnc in remain largely unknown. Here we found a noncoding termed Sch LAH (seven chromosome locus associated with ; also called BC 035072) was generally downregulated . Low expression significantly correlated shorter overall survival patients. In vitro vivo assays overexpression...
Background/Aims: Non-small cell lung carcinoma (NSCLC) is the most common type of cancer and cause cancer-related deaths. The molecular mechanisms that are involved in NSCLC development currently not well understood. Accumulating evidence shows histone demethylases play important roles regulation pathological developmental processes many diseases, including various types cancers. Methods: Mitochondrial membrane potential assays, migration invasion caspase-3 caspase-9 activity assays western...
Circulating tumor cells (CTCs) or circulating DNA (ctDNA) may be used for diagnostic prognostic purposes in patients with hepatocellular carcinoma (HCC). We aim to determine whether CTCs ctDNA are suitable oncogenic mutations HCC patients. Twenty-six mostly advanced were enrolled. 30 mL peripheral blood from each patient was obtained. CellSearch system CTC detection. A sequencing panel covering 14 cancer-relevant genes identify mutations. TERT promoter C228T and C250T determined by droplet...
High recurrence rates after resection of hepatocellular carcinoma (HCC) with curative intent impair clinical outcomes HCC. Cancer/testis antigens (CTAs) are suitable targets for cancer immunotherapy if selectively expressed in tumor cells. The aims were to identify CTAs that frequently and HCC-tumors, investigate whether could serve as biomarkers occult metastasis. Tumor paired tumor-free liver (TFL) tissues HCC-patients healthy assessed mRNA expression 49 by RT-qPCR protein five...
Abstract Background: High recurrence rates after resection of hepatocellular cancer (HCC) with curative intent and lack effective therapy for advanced disease impair clinical outcomes HCC. Cancer/testis antigens (CTAs) are suitable targets immunotherapy if selectively expressed in tumor cells. The aims this study were to establish a panel CTAs that frequently tumors HCC-patients, investigate whether might be tumor-free liver tissues HCC-patients. Methods: Surgically-resected paired (TFL) HCC...