A5017001558- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Skin and Cellular Biology Research
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Neurological disorders and treatments
- Nuclear Structure and Function
- Occupational exposure and asthma
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Parkinson's Disease Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Genetics, Bioinformatics, and Biomedical Research
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Cardiomyopathy and Myosin Studies
- Hippo pathway signaling and YAP/TAZ
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Occupational and environmental lung diseases
- RNA Research and Splicing
Åbo Akademi University
2024
Tel Aviv University
2019-2024
Philipps University of Marburg
2018
In life sciences, tracking objects from movies enables researchers to quantify the behavior of single particles, organelles, bacteria, cells, and even whole animals. While numerous tools now allow automated video, a significant challenge persists in compiling, analyzing, exploring large datasets generated by these approaches. Here, we introduce CellTracksColab, platform tailored simplify exploration analysis cell data. CellTracksColab facilitates compiling results across multiple fields...
The potential of microtubule-associated protein targets for cancer therapeutics remains largely unexplored due to the lack target-specific agents. Here, we explored therapeutic targeting cytoskeleton-associated 5 (CKAP5), an important protein, with CKAP5-targeting siRNAs encapsulated in lipid nanoparticles (LNPs). Our screening 20 solid cell lines demonstrated selective vulnerability genetically unstable response CKAP5 silencing. We identified a highly responsive chemo-resistant ovarian...
ABSTRACT Planar cell polarity (PCP) is essential for tissue morphogenesis and homeostasis; however, the mechanisms that orchestrate shape packing dynamics required to establish PCP are poorly understood. Here, we identified a major role globular (G)-actin-binding protein thymosin-β4 (TMSB4X) in establishment adhesion developing epidermis. Depletion of Tmsb4x mouse embryos hindered eyelid closure hair-follicle angling owing defects. depletion did not preclude epidermal vivo or vitro; it...
Contractile arrays of actin and myosin II filaments drive many essential processes in nonmuscle cells, including migration adhesion. Sequential organization along one dimension is followed by expansion into a two-dimensional network parallel actomyosin fibers, which are aligned to form stacks. The process stack formation has been studied detail. However, factors that oppose have not yet described. Here, we show tropomyosins act as negative regulators formation. Knockdown any or all...
De novo formation of epithelial cell-cell contacts relies on actin-based protrusions as well tightly controlled turnover junctional actin once cells encounter each other and adhesion complexes assemble. The specific contributions individual regulators either protrusion or remain largely unexplored. Based our previous findings Formin-like 2 (FMNL2)-mediated control dynamics, we investigated its potential role in membrane impact newly forming contacts. CRISPR/Cas9-mediated loss FMNL2 human...
Abstract Biallelic pathogenic variants in PRKN (PARK2), encoding the E3 ubiquitin ligase parkin, lead to early-onset Parkinson's disease. Structural variants, including duplications or deletions, are common due their location within fragile site FRA6E. These readily detectable by copy number variation analysis. We studied four siblings with levodopa-responsive dystonia exome sequencing followed genome sequencing. Affected individuals developed juvenile subsequent appearance of parkinsonism...
Abstract In life sciences, tracking objects from movies enables researchers to quantify the behavior of single particles, organelles, bacteria, cells, and even whole animals. While numerous tools now allow automated video, a significant challenge persists in compiling, analyzing, exploring large datasets generated by these approaches. Here, we introduce CellTracksColab, platform tailored simplify exploration analysis data. CellTracksColab facilitates compiling results across multiple fields...
The term 'fibroblast' covers a heterogeneous cell population in idiopathic pulmonary fibrosis (IPF). fibroblasts are considered as main effector cells, because they promote disease progression by releasing exaggerated amounts of extracellular matrix proteins and modifying microenvironment. As IPF-derived human lung (IPF-HLFs) were shown to express higher levels integrin alpha-5 (ITGA5) than normal derived HLFs (N-HLFs), we explored the importance ITGA5 IPF progression.IPF-HLF N-HLF primary...
Focal adhesion kinase (FAK) is well established as a regulator of cell migration, but whether and how the closely related proline-rich tyrosine 2 (Pyk2) regulates fibroblast motility still under debate. Using mouse embryonic fibroblasts (MEFs) from Pyk2-/- mice, we show here, for first time, that lack Pyk2 significantly impairs both random directed motility. MEFs reduced cell-edge protrusion dynamics, which dependent on protein-protein binding activities Pyk2. bioinformatics analysis in...
Abstract Cell mitosis is an attractive target for cancer therapy. Presently, anti-mitotic drugs are limited to tubulin binding agents and kinase inhibitors. While there a long list of potential microtubule associated protein targets that play important role in function, their therapeutic remains largely unexplored due the lack specific agents. Herein, we explored targeting Cytoskeleton Associated Protein 5 (CKAP5), microtubule-associated protein, with CKAP5 siRNAs entrapped lipid...