A5062174975- Microtubule and mitosis dynamics
- Hair Growth and Disorders
- Skin and Cellular Biology Research
- Psoriasis: Treatment and Pathogenesis
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Wound Healing and Treatments
- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Photosynthetic Processes and Mechanisms
- Stress Responses and Cortisol
- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Wnt/β-catenin signaling in development and cancer
- Plant Molecular Biology Research
- Dermatology and Skin Diseases
- Adipose Tissue and Metabolism
- Inflammatory Bowel Disease
- Mitochondrial Function and Pathology
- Melanoma and MAPK Pathways
- Dermatologic Treatments and Research
- Gene expression and cancer classification
- Mesenchymal stem cell research
- Fibroblast Growth Factor Research
Johnson & Johnson (United States)
2025
Janssen (United States)
2023-2024
Rockefeller University
2012-2016
Howard Hughes Medical Institute
2013-2015
University of Virginia
2009
University of Virginia Medical Center
2005-2008
Hair follicles (HFs) undergo cyclical periods of growth, which are fueled by stem cells (SCs) at the base resting follicle. HF-SC formation occurs during HF development and requires transcription factor SOX9. Whether how SOX9 functions in maintenance remain unknown. By conditionally targeting Sox9 adult HF-SCs, we show that is essential for maintaining them. SOX9-deficient HF-SCs still transition from quiescence to proliferation launch subsequent hair cycle. However, once activated, bulge...
Significance Signs of aging often first appear in our skin and hair. As animals age, hair follicles spend more time resting instead generating Here we show that this decline is rooted age-related changes systemic, local, intrinsic factors, which collaborate to reduce follicle stem cell (HFSC) activity. We uncover a unique hitherto-undescribed role for bone morphogenic protein signaling downstream effector, nuclear factor activated T-cell c1 (NFATc1). In young cells, NFATc1 on when they are...
How to grow hair or sweat glands Unlike other mammals that must pant seek shade water when overheated, humans are able self-cool tolerate extreme heat. Sweat glands, which enable run in marathons, instrumental for this remarkable feat. Lu et al. investigated skin appendage diversity during development of the furry backs and sweaty paws mice (see Perspective by Lai Chuong). They also examined human skin, is capable making both hairs same area body. Epithelialmesenchymal interactions, with...
IL-23 is implicated in the pathogenesis of immune-mediated inflammatory diseases, and myeloid cells that express Fc gamma receptor 1 (FcγRI or CD64) on their surface have been recently identified as a primary source inflamed tissue. Our complementary analyses transcriptomic datasets from psoriasis IBD showed increased expression CD64 transcripts tissue, greater abundance cell types with co-expression IL-23. These findings led us to explore potential implications binding function...
Tumor-initiating stem cells (SCs) exhibit distinct patterns of transcription factors and gene expression compared to healthy counterparts. Here, we show that dramatic shifts in large open-chromatin domain (super-enhancer) landscapes underlie these differences reflect tumor microenvironment. By vivo super-enhancer transcriptional profiling, uncover a dynamic cancer-specific epigenetic network selectively enriched for binding motifs factor cohort expressed squamous cell carcinoma SCs...
Behavior and physiology are essential readouts in many studies but have not benefited from the high-dimensional data revolution that has transformed molecular cellular phenotyping. To address this, we developed an approach combines commercially available automated phenotyping hardware with a systems biology analysis pipeline to generate readout of mouse behavior/physiology, as well intuitive health-relevant summary statistics (resilience biological age). We used this platform longitudinally...
The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined genetic interactions with four genes identify nonessential involved in chromosome segregation, individual roles of within checkpoint, and reveal potential complexity that may exist. used synthetic array (SGA) analysis using mutants mad1, mad2, mad3, bub3. found 228 substantial overlap spectrum between In contrast, there were many common bub3 not shared by mad3. pairs mutants,...
IL-23 is a cytokine produced by myeloid cells that drives the T helper 17 pathway and plays an essential role in pathophysiology of plaque psoriasis. activation initiates cascade cytokines subsequently inducing expression many psoriasis-related proteins. This study aimed to better understand underlying mechanisms driving differences between IL-17A blockade patients with psoriasis their implications for durability clinical responses. Serum and/or skin biopsies were isolated from treated...
The N-terminal tail of Ndc80 is essential for kinetochore-microtubule binding in human cells but not required viability yeast. We show that the yeast timely mitotic progression and accurate chromosome segregation. when are limited Dam1, demonstrating a redundant function Dam1 complexes vivo.
Abstract The anaphase promoting complex (APC) targets proteins for degradation to promote progression through the cell cycle. Here we show that Clb5, an APCCdc20 substrate, is degraded when spindle checkpoint active, while other substrates are stabilized, suggesting inhibition by substrate specific.
There is evidence accumulating for nonrandom segregation of one or more chromosomes during mitosis in different cell types. We use synchrony and two methods to show that all chromatids budding yeast segregate randomly there no mother-daughter bias with respect Watson Crick-containing strands DNA.
Abstract Behavior and physiology are essential readouts in many studies but have not benefited from the high-dimensional data revolution that has transformed molecular cellular phenotyping. To address this, we developed an approach combines commercially available automated phenotyping hardware with a systems biology analysis pipeline to generate readout of mouse behavior/physiology, as well intuitive health-relevant summary statistics (resilience biological age). We used this platform...
<h3>Background:</h3> IL-23 is implicated in the pathogenesis of psoriasis (PsO), and myeloid cells that express FcγRI, known as CD64, have been identified primary cellular source lesional PsO skin tissue.[1] The incidence prevalence psoriatic arthritis increases with severity PsO,[2] joint disease activity positively correlated frequency peripheral CD64<sup>+</sup> monocytes.[3] Guselkumab (GUS) risankizumab (RZB) are monoclonal antibodies (mAbs) specifically directed against IL-23p19...
Abstract Tumor-initiating cells within a cancer exhibit distinct patterns of transcription factors and gene expression compared to stem their healthy tissue. Little is known about the key that dictate chromatin remodeling accompanying transcriptional changes ultimately hardwire malignant behavior tumor-initiating cells. Here, by in vivo profiling, we show dramatic shifts large open-chromatin (super-enhancer) landscapes underlie these differences. Focusing on one most common life-threatening...