A5039286227- Immunotherapy and Immune Responses
- Blood transfusion and management
- Xenotransplantation and immune response
- Immune Response and Inflammation
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- T-cell and B-cell Immunology
- Prostate Cancer Treatment and Research
- Acute Myeloid Leukemia Research
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Phagocytosis and Immune Regulation
- Organ Transplantation Techniques and Outcomes
- Autophagy in Disease and Therapy
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Blood groups and transfusion
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Cell death mechanisms and regulation
- Hemostasis and retained surgical items
National Specialized Hospital for Active Treatment of Hematologic Diseases
2009-2023
George Washington University
2002-2006
Washington University Medical Center
2002-2006
Washington University in St. Louis
2005
Massachusetts General Hospital
2002
University of Genoa
2002
Harvard University
2002
Biomedical Research Institute
2001
Naval Medical Research Command
1998
American Red Cross
1989-1995
Objective: Chronic myelogenous leukemia (CML) is a disease, that in cases with high leukocyte numbers, treated hydration and cytoreductive therapy. Materials Methods:A 20-year-old female patient, during routine examination for pregnancy, presented elevated white blood cells (WBC) count (over 275x109/L) splenomegaly without any complaints. The diagnosis of Ph-positive CML was confirmed by cytology PCR [presence p210 type BCR::ABL1 [e13a2 (b2a2)] transcripts. Since she her 6th month abortion...
Abstract To induce an immunogenic response in vivo, antigen‐presenting (stimulator) cell must present both antigen‐specific (class II MHC) and accessory signal to the CD4 T cell. Failure express has been shown vitro a state of specific unresponsiveness (anergy) We have that although stimulator cells blood continue class MHC molecules during refrigerated storage, their ability diminishes, reaching zero all units tested by about 13 days. This implies excess 2 weeks old should not alloimmunize...
Abstract Sertoli cells, can function as non-professional tolerogenic antigen-presenting cells and sustain the blood-testis barrier formed by their tight junctions. The NOD-like receptor family members NALP3 inflammasome play a key role in pro-inflammatory innate immunity signalling pathways. Limited data exist on NOD1 NOD2 expression human mouse cells. Currently, there is no or We found that primary pre-pubertal adult line, TLR4\NOD1 crosstalk converged NFκB activation elicited activation,...
Abstract The 1997–2005 tularemia outbreak in Bulgaria affected 285 people. Ten strains were isolated from humans, a tick, hare, and water. Amplified fragment length polymorphism typing of the present isolates strain 1962 suggests that new genetic variant caused outbreak.
Abstract Freshly drawn peripheral blood granulocytes, monocytes and NK cells express Fas‐L following activation. Fully functional soluble (sFas‐L) is released found in fresh plasma samples from healthy volunteers coupled to a binding factor (PFBF). In the absence of leukocytes undergo rapid apoptosis with 15% granulocytes 25% showing signs immediately separation. Apoptosis progresses during refrigerated storage even presence all disintegrate by day 15 storage. The increases rate apoptosis....
During cancer progression, the genome instability incurred rearrangement could possibly turn some of tumor suppressor micro‐RNAs into pro‐oncogenic ones. We aimed to investigate miR‐204 in context prostate progression using a cell line model different levels (LNCaP, PC3, VCaP and NCI H660), as demonstrated by availability ERG fusion. studied effect modulation on master transcription factors important for lineage development, differentiation bone marrow metastasis. followed c‐MYB, ETS1 RUNX2...
Study of Fas and ligand (Fas-L) expression, as well sFas-L release, by fresh human peripheral blood leukocytes.Flow cytometry, cytotoxicity, immunofluorescence staining smears. Western blotting.Granulocytes monocytes express a low level receptor, but no Fas-L. These cells, NK contain presynthesized depots Fas-L which they following activation brief storage (60 min) at room temperature or during separation from whole blood. Such also leads to receptor upregulation. cells do not receptor. Once...
Two separate signals are required for induction of T cell proliferation. In an attempt to identify them we used polyclonal activation with Con A, which requires costimulation autologous accessory cells. The co-stimulatory activity is not constitutively expressed on cells since such fixed immediately after separation from whole blood unable provide the necessary signal(s), although readily by incubation and subsequently will support A-induced Addition recombinant IL-1 plus IL-6 cultures in...
Abstract Objectives : Study of Fas and ligand(Fas‐L) expression, as well sFas–L release, by fresh human peripheral blood leukocytes. Methods Flow cytometry, cytotoxicity, immunofluorescence staining smears, Western blotting, Results Granulocytes monocytes express a low level receptor, but no Fas–L. These cells, NK contain presynthesized depots Fas–L which they following activation brief storage (60 min) at room temperature or during separation from whole blood. Such also leads to receptor...
During refrigerated storage leukocytes in donor blood progressively undergo apoptosis followed by secondary necrosis. Using an inbred rodent transfusion model, recipient animals received viable, necrotic, or apoptotic cells. While of viable MNCs stimulated production IgM, IgG1 (Th2 type) and IgG2a (Th1-type) antidonor antibodies, leading to a suppression subsequent DTH antigens, cells led neither alloimmunization nor immunosuppression. On the other hand lysed resulted IgM (Th2-type)...
Treatment of stimulator cells with 0.1% paraformaldehyde for 60 sec or ultraviolet-B (UV-B) irradiation (1000 J/m) eliminates their ability to elicit T cell pro liferation in a primary mixed leukocyte reaction. How ever, response equal 20–40% control value could be elicited by fixed UV-B irradiated providing the latter are incubated at 37°C 18 hr prior treatment. The incubation also induces one-log increase density fluorescence when stained monoclonal antibodies against class II molecules DR...
To induce an immunogenic response in vivo, antigen-presenting (stimulator) cell must present both antigen-specific (class II MHC) and accessory signal to the CD4 T cell. Failure express has been shown vitro a state of specific unresponsiveness (anergy) We have that although stimulator cells blood continue class MHC molecules during refrigerated storage, their ability diminishes, reaching zero all units tested by about 13 days. This implies excess 2 weeks old should not alloimmunize but could...