A5045757553- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Mass Spectrometry Techniques and Applications
- Plant Reproductive Biology
- Insect and Pesticide Research
- Macrophage Migration Inhibitory Factor
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Protein Kinase Regulation and GTPase Signaling
- Marine and coastal ecosystems
- Microbial bioremediation and biosurfactants
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Toxin Mechanisms and Immunotoxins
- Microbial Metabolic Engineering and Bioproduction
- Venomous Animal Envenomation and Studies
- Hydrocarbon exploration and reservoir analysis
- Neurobiology and Insect Physiology Research
- Galectins and Cancer Biology
- Petroleum Processing and Analysis
- Enhanced Oil Recovery Techniques
- Microtubule and mitosis dynamics
- Colorectal Cancer Screening and Detection
University of Essex
2015-2025
Genomics (United Kingdom)
2024
Medical University Pleven
2024
Inserm
2024
Institut des Maladies Génétiques Imagine
2024
Inspection Technologies (United Kingdom)
2023
Colchester Hospital
2016
University of Illinois Chicago
2001-2013
Park University
2012
University of Oxford
2011
Drugs that inhibit estrogen receptor-α (ER) activity have been highly successful in treating and reducing breast cancer progression ER-positive disease. However, resistance to these therapies presents a major clinical problem. Recent genetic studies shown mutations the ER gene are found >20% of tumours progress on endocrine therapies. Remarkably, great majority localize just few amino acids within or near critical helix 12 region hormone binding domain, where they likely be single allele...
A Calvin cycle multiprotein complex including phosphoribulokinase (PRK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and a small protein, CP12, has previously been identified. In this article, we have studied in leaves shown that dissociation reassociation of the PRK/GAPDH/CP12 occurs time frame minutes, allowing for rapid regulation enzyme activity. Furthermore, extent formation correlates with quantity light. These data provide evidence linking status subtle GAPDH PRK activities...
Summary Mechanistic understanding of the costs and benefits photoacclimation requires knowledge how photophysiology is affected by changes in molecular structure chloroplast. We tested hypothesis that light dependencies photosynthesis, nonphotochemical quenching PSII photoinactivation arises from abundances chloroplast proteins Emiliania huxleyi strain CCMP 1516 grown at 30 (Low Light; LL ) 1000 (High HL μmol photons m −2 s −1 photon flux densities. Carbon‐specific light‐saturated gross...
Covalent hemoglobin binding to membranes leads band 3 (AE1) clustering and the removal of erythrocytes from circulation; it is also implicated in blood storage lesions. Damaged hemoglobin, with heme being a redox oxygen-binding inactive hemichrome form, has been as species. However, previous studies used strong non-physiological oxidants. In vivo constantly oxidised methemoglobin (ferric), around 1% this form at any one time. study we tested ability natural (methemoglobin) presence or...
During mating, budding yeast cells reorient growth toward the highest concentration of pheromone. Bni1p, a formin homologue, is required for this polarized by facilitating cortical actin cable assembly. Fus3p, pheromone-activated MAP kinase, pheromone signaling and cell fusion. We show that Fus3p phosphorylates Bni1p in vitro, phosphorylation vivo during response dependent on Fus3p. fus3 mutants exhibited multiple phenotypes similar to bni1 mutants, including defects polarization, as well...
The mating response of the budding yeast Saccharomyces cerevisiae is mediated by a prototypical heterotrimeric GTP-binding protein (G protein) and mitogen-activated kinase (MAPK) cascade. Although signal transmission such pathways has been modeled in detail, postreceptor down-regulation less well understood. pheromone-responsive G α subunit (Gα) down-regulates signal, but its targets are unknown. We have found that Gα binds directly to mating-specific MAPK cells responding pheromone. This...
Limitation of marine primary production by the availability nitrogen or phosphorus is common. Emiliania huxleyi, a ubiquitous phytoplankter that plays key roles in production, calcium carbonate precipitation and dimethyl sulfide, often blooms mid-latitude at beginning summer when inorganic nutrient concentrations are low. To understand physiological mechanisms allow such blooms, we examined how proteome E. huxleyi (strain 1516) responds to N P limitation. We observed modest changes much...
Microarray technology, as well other functional genomics experiments, allow simultaneous measurements of thousands genes within each sample. Both the prediction accuracy and interpretability a classifier could be enhanced by performing classification based only on selected discriminative genes. We propose statistical method for selecting overlapping analysis expression data across classes. This results in novel measure, called proportional score (POS), feature’s relevance to task. apply POS,...
MGVB is a collection of tools for proteomics data analysis. It covers processing from in silico digestion protein sequences to comprehensive identification post-translational modifications and solving the inference problem. The toolset developed with efficiency mind. enables analysis at fraction resources cost typically required by existing commercial free tools. MGVB, as it native application, faster than such MaxQuant and, same time, finds very similar, some cases even larger, numbers...
We have conducted proteome-wide analysis of fresh surgery specimens derived from breast cancer patients, using an approach that integrates size-based intact protein fractionation, nanoscale liquid separation peptides, electrospray ion trap mass spectrometry, and bioinformatics. Through this approach, we acquired a large amount peptide fragmentation spectra size-resolved fractions the proteomes several tumors, tissue peripheral to tumor, samples patients undergoing noncancer surgery....
The marine obligate hydrocarbonoclastic bacterium Thalassolituus oleivorans MIL-1 metabolizes a broad range of aliphatic hydrocarbons almost exclusively as carbon and energy sources. We used LC-MS/MS shotgun proteomics to identify proteins involved in aerobic alkane degradation during growth on medium- (n-C14) or long-chain (n-C28) alkanes. During n-C14, T. expresses an monooxygenase system terminal oxidation including two 1-monooxygenases, ferredoxin, ferredoxin reductase aldehyde...
Abstract Sertoli cells, can function as non-professional tolerogenic antigen-presenting cells and sustain the blood-testis barrier formed by their tight junctions. The NOD-like receptor family members NALP3 inflammasome play a key role in pro-inflammatory innate immunity signalling pathways. Limited data exist on NOD1 NOD2 expression human mouse cells. Currently, there is no or We found that primary pre-pubertal adult line, TLR4\NOD1 crosstalk converged NFκB activation elicited activation,...
In cold marine environments, the obligate hydrocarbon-degrading psychrophile Oleispira antarctica RB-8, which utilizes aliphatic alkanes almost exclusively as substrates, dominates microbial communities following oil spills. this study, LC-MS/MS shotgun proteomics was used to identify changes in proteome induced during growth on n-alkanes and temperatures. Specifically, proteins with significantly higher relative abundance tetradecane (n-C14 ) at 16°C 4°C have been quantified. During n-C14 ,...
Abstract The thioredoxin-regulated chloroplast protein CP12 forms a multienzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK GAPDH are inactivated when present in this complex, process shown vitro to be dependent upon oxidized CP12. importance of vivo higher plants, however, has not been investigated. Here, antisense suppression tobacco (Nicotiana tabacum) was observed impact on NAD-induced formation but had...
The γ subunit of the major histocompatibility complex (MHC) class II complex, CD74, is overexpressed in a significant proportion metastatic breast tumors, but mechanistic foundation and biologic significance this phenomenon are not fully understood. Here, we show that when CD74 human cancer noncancerous epithelial cells, it interacts interferes with function Scribble, product well-known tumor suppressor gene. Furthermore, using cell lines expressing under control tetracycline-inducible...
Summary Optimality principles are often applied in theoretical studies of microalgal ecophysiology to predict changes allocation resources different metabolic pathways, and optimal acclimation is likely involve the proteome, which typically accounts for > 50% cellular nitrogen (N). We tested hypothesis that microalga Emiliania huxleyi CCMP 1516 suboptimal vs supraoptimal light involves large proteome as cells rebalance capacities absorb light, fix CO 2 , perform biosynthesis resist...
Summary Alcanivorax borkumensis SK2 T is an important obligate hydrocarbonoclastic bacterium (OHCB) that can dominate microbial communities following marine oil spills. It possesses the ability to degrade branched alkanes which provides it a competitive advantage over many other alkane degraders only linear alkanes. We used LC–MS/MS shotgun proteomics identify proteins involved in aerobic degradation during growth on ( n ‐C 14 ) or (pristane) During , A. expressed complete pathway for...
Protein phosphorylation is a ubiquitous post-translational modification that affects significant subset of the proteome and plays an especially important role in signal transduction cell cycle control eukaryotic organisms. Recently developed methods couple multidimensional liquid chromatography to electrospray mass spectrometers can be used analyze entire phosphoproteomes. However, they require considerable investments technical skills are only available few highly specialized laboratories....
Abstract Purpose: Brother of the regulator imprinted sites (BORIS) is a novel member cancer-testis antigen gene family. These genes are normally expressed only in spermatocytes but abnormally activated different malignancies, including breast cancer. The aim this study was to investigate expression BORIS leukocytes cancer patients and correlation between levels clinical/pathologic variables. Experimental Design: Leukocytes were obtained from whole blood 87 52 donors not diagnosed with...
Major histocompatibility complex (MHC) class II-associated antigen presentation involves an array of interacting molecules. CD74, the cell surface isoform MHC invariant chain, is one such molecule; its role remains poorly defined. To address this, we have employed a high-resolution single-particle imaging method for quantifying colocalization CD74 with human leukocyte (HLA)-DR molecules on fibroblast cells known their capacity to function as antigen-presenting cells. We also examined whether...
We used high-resolution mass spectrometry to measure the abundance of more than 9,000 proteins in 19 individually dissected colorectal tumors representing lymph node metastatic (n = 10) and nonmetastatic 9) phenotypes. Statistical analysis identified MX1 several other as overexpressed node-positive tumors. MX1, IGF1-R IRF2BP1 showed significantly different expression immunohistochemical validation (Wilcoxon test p 0.007 for IGF1-R, 0.04 0.02 at invasion front) cohort. Knockout by siRNA cell...
During cancer progression, the genome instability incurred rearrangement could possibly turn some of tumor suppressor micro‐RNAs into pro‐oncogenic ones. We aimed to investigate miR‐204 in context prostate progression using a cell line model different levels (LNCaP, PC3, VCaP and NCI H660), as demonstrated by availability ERG fusion. studied effect modulation on master transcription factors important for lineage development, differentiation bone marrow metastasis. followed c‐MYB, ETS1 RUNX2...