A5022654896- Advanced Proteomics Techniques and Applications
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Nutrition, Genetics, and Disease
- BRCA gene mutations in cancer
- Nanoplatforms for cancer theranostics
- RNA Research and Splicing
- Macrophage Migration Inhibitory Factor
- Stress Responses and Cortisol
- Biosensors and Analytical Detection
- Meta-analysis and systematic reviews
- Medical Imaging Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Prion Diseases and Protein Misfolding
- Signaling Pathways in Disease
- CRISPR and Genetic Engineering
- Health, psychology, and well-being
- Cancer-related Molecular Pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Cell Adhesion Molecules Research
- Tryptophan and brain disorders
- Advanced Chemical Physics Studies
- Breast Cancer Treatment Studies
- Galectins and Cancer Biology
Anglia Ruskin University
2007-2016
Cancer Research UK
2010
University of Cambridge
2001-2009
Zero to Three
2009
University of Essex
2008
Broomfield Hospital
2008
Chelmsford and Essex Hospital
2008
The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency known be variable, little attention has been paid the practical implications that variability.We investigated reproducibility with commercial transcriptases samples variable quality concentration. We quantified several mRNA targets either singleplex SYBR Green I or dualplex probe-based real-time quantitative PCR (RT-qPCR), latter used...
ZnO nanoparticles (NPs) are reported to show a high degree of cancer cell selectivity with potential use in imaging and therapy. Questions remain about the mode by which NPs cause death, whether they exert an intra‐ or extracellular effect, resistance among different types NP exposure. The present study quantifies variability between cellular toxicity, dynamics uptake, dissolution bare RGD (Arg‐Gly‐Asp)‐targeted MDA‐MB‐231 cells. Compared NPs, RGD‐targeting integrin αvβ3 receptors expressed...
We have conducted proteome-wide analysis of fresh surgery specimens derived from breast cancer patients, using an approach that integrates size-based intact protein fractionation, nanoscale liquid separation peptides, electrospray ion trap mass spectrometry, and bioinformatics. Through this approach, we acquired a large amount peptide fragmentation spectra size-resolved fractions the proteomes several tumors, tissue peripheral to tumor, samples patients undergoing noncancer surgery....
Retrospective studies on male breast cancer (MBC) have suff ered from small numbers of cases available any one centre; thus a signifi cant problem in eff ectively studying this disease is accruing suffi ciently large to allow comparative analysis biomarkers associated with response.Using coordinated multicentre approach, we present the fi rst large-scale study address relevance expression hormone receptors MBC and female (FBC) using immunohistochemistry combined novel bioinformatics...
The γ subunit of the major histocompatibility complex (MHC) class II complex, CD74, is overexpressed in a significant proportion metastatic breast tumors, but mechanistic foundation and biologic significance this phenomenon are not fully understood. Here, we show that when CD74 human cancer noncancerous epithelial cells, it interacts interferes with function Scribble, product well-known tumor suppressor gene. Furthermore, using cell lines expressing under control tetracycline-inducible...
Application of allostatic theory to stress during the 1990s refocused attention on internal responses a perceived hazard, and last 20 years has seen considerable developments in biological contexts stress. Evidence from neuroscience now suggests that secretion hormone cortisol is not only stimulated by outcomes cognitive transaction but it also feeds back contributes positively adaptation feature resilience. More recently, operative intracellular mechanisms are beginning be understood...
The proximity ligation assay (PLA) detects proteins via their interaction with pairs of probes, which are antibodies coupled to noncomplementary DNA oligonucleotides. binding both probes epitopes on the target protein brings oligonucleotides together, allowing them be bridged by a third oligonucleotide complementarity other two. This enables and detection resulting amplicon real-time quantitative PCR (qPCR), acts as surrogate marker for interest. Hence PLA has potential clinically relevant...
We report an approach for multiplex analysis of cancer biomarkers based on the measurement diagnostic peptides in whole tissue protein digests. Label-free quantitation with MS3 multiple reaction monitoring (MRM) was developed to afford accurate prospective marker a panel breast tumors. This provides economical and robust alternative stable isotope-based methods. It is equally applicable samples derived from biopsy, aspirate, or plasma can be easily translated clinic.
Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge use tissue for proteomics studies the small sample size and difficulties extract preserve proteins. choice of buffer compatible with applications also challenge. Here we describe protocol optimized most efficient extraction proteins from human breast two-dimensional gel electrophoresis (2D-GE). This based on mechanically assisted disintegration tissues directly 2D-GE buffer....
The normal cellular form of the prion protein PrP(C) is a glycosylphosphatidylinositol-linked cell-surface glycoprotein expressed primarily by cells nervous and immune systems. There evidence to suggest that involved in cell signalling homeostasis. We have investigated composition peripheral lymphoid tissue PrP-/-, wild-type, tg19 tga20 strains mice, which express 0, 1-, 3-5- 4-7-fold higher levels PrP(C), respectively, relative wild-type mice. Our data show mice reduced number spleen T-cell...
On page 1310 J. S. Merzaban, A. E. Porter, and co-workers present fluorescently labeled RGD-targeted ZnO nanoparticles (NPs; green) for the targeted delivery of cytotoxic to integrin αvβ3 receptors expressed on triple negative breast cancer cells. Correlative light-electron microscopy shows that NPs dissolve into ionic Zn2+ (blue) upon uptake cause apoptosis (red) with intra-tumor heterogeneity, thereby providing a possible strategy therapy. Cover design by Ivan Gromicho.