A5005576901- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- HIV/AIDS drug development and treatment
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- DNA Repair Mechanisms
- HIV Research and Treatment
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Genetic Neurodegenerative Diseases
- Tumors and Oncological Cases
University of Science and Technology of China
2024
University of Houston
1997-2005
Oligonucleotide microarrays or oDNA chips are effective decoding and analytical tools for genomic sequences useful a broad range of applications. Therefore, it is desirable to have synthesis methods DNA that highly flexible in sequence design provide high quality general adoptability. We report herein, microarray based on biochip method. Our method simply uses photogenerated acid (PGA) solution trigger deprotection the 5′-OH group conventional nucleotide phosphoramidite monomers (i.e....
There is substantial interest in implementing a bioinformatics tool that allows the design of oligonucleotides to support development vitro gene synthesis. Current protocols make long synthetic DNA molecules rely on assembly set short oligonucleotides, either by ligase chain reaction (LCR) or PCR. Ideally, such should represent both strands final molecule. They be adjacent same strand and overlap complementary from second ensure good hybridization during assembly. This implies thermodynamic...
Abstract Spectroscopic, thermal denaturation and kinetic studies have revealed that DNA oligonucleotides 5′-d(GGGTGGGTGGGTGGGT) (T30695) 5′-d(GTGGTGGGTGGGTGGGT) (T30177) form extremely stable intramolecular G-tetrads via a two-step process involves the binding of one K+ ion to central pair G-quartets two additional ions, presumably, loops (Jing et al., (1997) Biochemistry in press). In these are potent HIV-1 inhibitors among most active integrase yet identified, we sought further...
Extrachromosomal circular DNA (eccDNA) is crucial in oncogene amplification, gene transcription regulation, and intratumor heterogeneity. While various analysis pipelines experimental methods have been developed for eccDNA identification, their detection efficiencies not systematically assessed. To address this, we evaluate the performance of 7 using seven simulated datasets, terms accuracy, identity, duplication rate, computational resource consumption. We also compare efficiency through...
The mechanism by which HIV-1 reverse transcriptase (HIV-RT) discriminates between the correct and incorrect nucleotide is not clearly understood. Chemically modified nucleotides containing 1-aminonaphthalene-5-sulfonate (ANS) attached to their γ-phosphate were synthesized used probe selection this error prone polymerase. Primer extension reactions provide direct evidence that polymerase able incorporate gamma-modified nucleotides. Forward mutation assays reveal a 6-fold reduction in...
Helix 42 of Domain II Escherichia coli 23S ribosomal RNA underlies the L7/L12 stalk in ribosome and may be significant positioning this feature relative to rest 50S subunit. Unlike Haloarcula marismortui Deinococcus radiodurans examples, lower portion helix E.coli contains two consecutive G•A oppositions with both adenines on same side stem. Herein, structure an analog positions 1037–1043 1112–1118 region is reported. NMR spectra calculations support a cis Watson–Crick/Watson–Crick (cis...