A5009166159- Stress Responses and Cortisol
- Immune Response and Inflammation
- Hormonal Regulation and Hypertension
- Adrenal Hormones and Disorders
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Blood groups and transfusion
- Neuroendocrine regulation and behavior
- Monoclonal and Polyclonal Antibodies Research
- Fatty Acid Research and Health
- Pneumocystis jirovecii pneumonia detection and treatment
- Chronic Lymphocytic Leukemia Research
- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- Immune responses and vaccinations
- GDF15 and Related Biomarkers
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
Novartis (United States)
2024
Boston University
2001-2003
Oregon Health & Science University
1996-2001
Colorado State University
2001
Salk Institute for Biological Studies
1992
We previously reported that gram-negative bacterial lipopolysaccharide (LPS) activates cells via Toll-like receptor (TLR) 4, whereas the mycobacterial cell wall glycolipid lipoarabinomannan (LAM) TLR2. also identified a secreted TLR2 agonist activity in short-term culture filtrates of Mycobacterium tuberculosis bacilli, termed soluble factor (STF). Here we show STF contains mannosylated phosphatidylinositol (PIM) and purified PIM possesses activity. Stimulation RAW 264.7 macrophages by LPS,...
Abstract Toll-like receptor (TLR) proteins mediate cellular activation by microbes and microbial products. To delineate the role of TLR in development host immune responses against mycobacteria, wild-type TLR-deficient mice were infected with nonpathogenic Mycobacterium bovis bacillus Calmette-Guerin (BCG). Two weeks after intraperitoneal challenge BCG, few bacilli present lungs TLR4−/− mice, whereas bacterial loads tenfold higher TLR2−/− mice. BCG vitro strongly induced proinflammatory...
BACKGROUND Mammalian Toll-like receptor (TLR) proteins are pattern recognition receptors for a diverse array of bacterial and viral products. Gram negative lipopolysaccharide (LPS) activates cells through TLR4, whereas the mycobacterial cell wall glycolipids, lipoarabinomannan (LAM) mannosylated phosphatidylinositol (PIM), activate TLR2. Furthermore, short term culture filtrates M tuberculosis bacilli contain TLR2 agonist activity, termed soluble factor (STF), that appears to be PIM. It was...
In mammals, the release of pituitary ACTH is stimulated by CRF. Two related peptides exist in nonmammalian vertebrates, sauvagine from frog skin and urotensin-I urophysis teleost fish. Their structures (approximately 50%) capacity to stimulate mammalian fish pituitaries has led proposal that are homologs However, does not appear amphibians, although CRF (ovine) induces a potent response amphibian pituitaries. This could indicate main function involve regulation suggests an additional...
Growth Differentiation Factor 15 (GDF15), a divergent member of the TGF-β superfamily, signals via hindbrain glial-derived neurotrophic factor receptor alpha-like and rearranged during transfection co-receptor (GFRAL-RET) complex. In nonclinical species, GDF15 is potent anorexigen leading to substantial weight loss. MBL949 half-life extended recombinant human dimer.
CRH is the principal mediator of stress response in mammals. In addition to pituitary and central nervous system effects, peripheral effects have been observed involving immune cardiovascular systems. Two receptor subtypes, CRH-R1 CRH-R2, cloned show significant amino acid homology (69%), but differ their tissue distribution. expressed predominantly brain pituitary, whereas CRH-R2 subtype highly heart skeletal muscle. To investigate role cardiac signaling, we analyzed effect on freshly...
Two receptors (CRH receptor type 1 and CRH 2) have been identified for the stress-induced neuropeptide, related peptides, urocortin, urocortin II. We previously found marked down-regulation of cardiac 2 expression following administration bacterial endotoxin, lipopolysaccharide, a model systemic immune activation, inflammation. postulated that inflammatory cytokines may regulate 2. show IL-1α significantly down-regulates mRNA in mouse heart. In addition, TNFα treatment also reduces...
The extent to which somatic mutation impairs the Ig complementarity-determining region (CDR) and framework (FRW) structure/function is not clear. Previously, we found that VH CDR2 of murine T15 Ab highly sensitive mutation; 56% (26 46) Abs mutated in vitro had reduced or no Ag binding capability, 9% were secretion impaired. Here test whether structure unique by mutating anti-PC-protein Ab, PCG1-1. PCG1-1 encoded M141 gene unrelated sequence VH1. majority (54%, 20 37) mutants carrying one...
CRH is the principal mediator of stress response in mammals. In addition to pituitary and central nervous system effects, peripheral effects have been observed involving immune cardiovascular systems. Two receptor subtypes, CRH-R1 CRH-R2, cloned show significant amino acid homology (69%), but differ their tissue distribution. expressed predominantly brain pituitary, whereas CRH-R2 subtype highly heart skeletal muscle. To investigate role cardiac signaling, we analyzed effect on freshly...