Wendy Stevens
A5101703309- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Transgenic Plants and Applications
- HIV/AIDS Research and Interventions
- vaccines and immunoinformatics approaches
- Single-cell and spatial transcriptomics
- HIV/AIDS drug development and treatment
- Cancer Genomics and Diagnostics
- Viral Infections and Outbreaks Research
University of the Witwatersrand
2020-2022
National Health Laboratory Service
2020
Abstract The SARS-CoV-2 epidemic in southern Africa has been characterized by three distinct waves. first was associated with a mix of lineages, while the second and third waves were driven Beta (B.1.351) Delta (B.1.617.2) variants, respectively 1–3 . In November 2021, genomic surveillance teams South Botswana detected new variant rapid resurgence infections Gauteng province, Africa. Within days genome being uploaded, it designated concern (Omicron, B.1.1.529) World Health Organization and,...
Background : The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has swept the world and poses a significant global threat to lives livelihoods, with 115 million confirmed cases at least 2.5 deaths from Coronavirus disease 2019 (COVID-19) in first year of pandemic. Developing tools measure seroprevalence understand protective immunity SARS-CoV-2 is priority. We aimed develop serological assay using plant-derived recombinant viral proteins, which represent important...
Abstract Background Circulating tumor DNA present in cell-free (cfDNA) serves as diagnostic, predictive, and prognostic markers cancer patients. In lymphoma patients, clonal immunoglobulin (cIg) sequences enable accurate identification of disease states. Despite its importance, there is sparse literature surrounding the preanalytical analytical conditions that affect cIg detection cfDNA. This study evaluates effects cfDNA isolation next-generation sequencing (NGS) library preparation on IGH...
Clonal immunoglobulin and T-cell receptor rearrangements serve as tumor-specific markers that have become mainstays of the diagnosis monitoring lymphoid malignancy. Next-generation sequencing (NGS) techniques targeting these loci been successfully applied to lymphoblastic leukemia multiple myeloma for minimal residual disease detection. However, adoption NGS primary remains limited.
The tiered laboratory framework for HIV viral load monitoring accommodates a range of testing platforms, with quality assessment critical to ensure patient testing. plasma is challenged by the instability RNA. An approach using an RNA stabilizing buffer described Xpert HIV-1 Viral Load (Cepheid) assay and was tested in remote laboratories South Africa. EDTA-plasma panels known titres prepared PrimeStore molecular transport medium per-module verification per-instrument external assessment....