Zinhle Makatini
A5080397482- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- HIV/AIDS Research and Interventions
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Animal Virus Infections Studies
- HIV/AIDS drug development and treatment
- COVID-19 epidemiological studies
- HIV/AIDS Impact and Responses
- HIV, Drug Use, Sexual Risk
- vaccines and immunoinformatics approaches
- Bacillus and Francisella bacterial research
- Chronic Disease Management Strategies
- COVID-19 diagnosis using AI
- Influenza Virus Research Studies
- Autoimmune and Inflammatory Disorders
- Clinical Laboratory Practices and Quality Control
- Syphilis Diagnosis and Treatment
- Infectious Diseases and Tuberculosis
- Adolescent Sexual and Reproductive Health
- HIV/AIDS oral health manifestations
- Viral gastroenteritis research and epidemiology
- Orthopedic Infections and Treatments
- Tuberculosis Research and Epidemiology
National Health Laboratory Service
2021-2024
University of the Witwatersrand
2019-2024
Johannesburg Hospital
2021-2024
Sefako Makgatho Health Sciences University
2019-2022
Royal Hallamshire Hospital
1999
Abstract The SARS-CoV-2 epidemic in southern Africa has been characterized by three distinct waves. first was associated with a mix of lineages, while the second and third waves were driven Beta (B.1.351) Delta (B.1.617.2) variants, respectively 1–3 . In November 2021, genomic surveillance teams South Botswana detected new variant rapid resurgence infections Gauteng province, Africa. Within days genome being uploaded, it designated concern (Omicron, B.1.1.529) World Health Organization and,...
Abstract Three lineages (BA.1, BA.2 and BA.3) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant concern predominantly drove South Africa’s fourth Coronavirus Disease 2019 (COVID-19) wave. We have now identified two new lineages, BA.4 BA.5, responsible for a fifth wave infections. The spike proteins BA.5 are identical, similar to except addition 69–70 deletion (present in Alpha BA.1 lineage), L452R Delta variant), F486V wild-type amino acid at Q493. differ...
ABSTRACT Background The SARS-CoV-2 Omicron variant of concern (VOC) almost completely replaced other variants in South Africa during November 2021, and was associated with a rapid increase COVID-19 cases. We aimed to assess clinical severity individuals infected Omicron, using S Gene Target Failure (SGTF) on the Thermo Fisher Scientific TaqPath PCR test as proxy. Methods performed data linkages for (i) laboratory tests, (ii) case data, (iii) genome (iv) DATCOV national hospital surveillance...
Abstract Omicron lineages BA.4 and BA.5 drove a fifth wave of COVID-19 cases in South Africa. Here, we use the presence/absence S-gene target as proxy for SARS-CoV-2 variant/lineage infections diagnosed using TaqPath PCR assay between 1 October 2021 26 April 2022. We link national individual-level data including case, laboratory test hospitalisation data. assess severity multivariable logistic regression comparing risk severe disease, once hospitalised, Delta, BA.1, BA.2 BA.4/BA.5...
The SARS-CoV-2 epidemic in southern Africa has been characterized by three distinct waves. first was associated with a mix of lineages, while the second and third waves were driven Beta (B.1.351) Delta (B.1.617.2) variants, respectively1–3. In November 2021, genomic surveillance teams South Botswana detected new variant rapid resurgence infections Gauteng province, Africa. Within days genome being uploaded, it designated concern (Omicron, B.1.1.529) World Health Organization and, within...
Abstract The Beta variant of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in South Africa late 2020 and rapidly became dominant variant, causing over 95% infections country during after second epidemic wave. Here we show rapid replacement by Delta a highly transmissible concern (VOC) that India subsequently spread around world. was imported to primarily from India, large monophyletic clusters all provinces, within three months introduction. This associated with...
Global genomic surveillance of SARS-CoV-2 has identified variants associated with increased transmissibility, neutralization resistance and disease severity. Here we report the emergence PANGO lineage C.1.2, detected at low prevalence in South Africa eleven other countries. The a high substitution rate, includes changes within spike protein that have been transmissibility or reduced sensitivity VOC/VOIs. Like Beta Delta, C.1.2 shows significantly to plasma from vaccinees individuals infected...
Abstract Global genomic surveillance of SARS-CoV-2 has identified variants associated with increased transmissibility, neutralization resistance and disease severity. Here we report the emergence PANGO lineage C.1.2, detected at low prevalence in South Africa eleven other countries. The initial C.1.2 detection is a high substitution rate, includes changes within spike protein that have been transmissibility or reduced sensitivity concern interest. Like Beta Delta, shows significantly to...
The circulation of Omicron BA.1 led to the rapid increase in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases South Africa November 2021, which warranted use more detection methods. We, therefore, assessed ability detect using genotyping assays identify specific mutations SARS-CoV-2 positive samples, Gauteng province, Africa. TaqPath™ COVID-19 real-time polymerase chain reaction assay was performed on all samples selected spike gene target failure (SGTF). were used for...
Summary The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic in southern Africa has been characterised by three distinct waves. first was associated with a mix of SARS-CoV-2 lineages, whilst the second and third waves were driven Beta Delta variants respectively 1–3 . In November 2021, genomic surveillance teams South Botswana detected new variant rapid resurgence infections Gauteng Province, Africa. Within days genome being uploaded, it designated concern (Omicron)...
The rapid emergence and spread of numerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants across the globe underscores crucial need for continuous SARS-CoV-2 surveillance to ensure that potentially more pathogenic are detected early contained. Whole genome sequencing (WGS) is currently gold standard COVID-19 surveillance; however, it remains cost-prohibitive requires specialized technical skills. To increase capacity, especially in resource-scarce settings,...
Since there are currently no specific SARS-CoV-2 prognostic viral biomarkers for predicting disease severity, has been interest in using polymerase chain reaction (PCR) cycle-threshold (Ct) values to predict progression.
Abstract Omicron lineages BA.4 and BA.5 drove a fifth wave of COVID-19 cases in South Africa. We assessed the severity BA.4/BA.5 infections using presence/absence S-gene target for diagnosed TaqPath PCR assay between 1 October 2021 26 April 2022. linked national individual-level data including case, laboratory test hospitalisation data. multivariable logistic regression comparing risk severe disease, once hospitalised, Delta, BA.1, BA.2 infections. After controlling factors associated with...
Background: Despite the growing evidence for reasonable acceptance and willingness to use HIV self-testing (HIVST), South Africa has not yet fully explored HIVST.Objective: This study’s objective was determine knowledge, attitudes, practices HIVST among students aged 18 29 years from University of Witwatersrand, Johannesburg. Methods: An online cross-sectional self-administered survey used collect data 01 January 2020 31 June 2020. Chi-squared test contribution between categorical variables...
Background. Large cohorts of HIV-1 perinatally infected children with long-term follow-up in developing countries are limited. Objectives. To explore rates and predictors virological failure a paediatric cohort. Methods. A 10-year retrospective study was conducted from January 2004 to December 2013 determine the incidence factors associated among 1 659 HIV public sector setting South Africa (SA). Children aged <17 years who initiated first-line antiretroviral therapy between 31 had at least...
Gauteng province (GP) was one of the most affected provinces in country during first two pandemic waves South Africa. We aimed to describe characteristics coronavirus disease 2019 (COVID-19) patients admitted largest quaternary hospitals GP waves.Study objectives were determine factors associated with hospital admission second wave and in-hospital COVID-19 mortality.Data from a national hospital-based surveillance system hospitalisations used. Multivariable logistic regression models...
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Advances in HIV management have improved treatment outcomes the HIV-infected population. However, these advances not been without multifaceted challenges. In sub-Saharan Africa, their impact is reflected increased emergence of drug resistance mutations. With rise exposure children to protease inhibitors (PIs), possibility increasing PI remains a concern.To describe group antiretroviral-experienced with mutations after failure on first- or second-line regimens public sector setting South...
In resource-limited settings, holding regimens such as lamivudine monotherapy (LAM) have been used to manage HIV-positive children failing combination antiretroviral therapy mitigate the risk of drug resistance developing, while adherence barriers are addressed or when access second- third-line is restricted. South African HIV treatment guidelines previously advocated use LAM HIV-infected with virological failure. However, outcomes patients on compared those who continued a regimen not well...