A5032816665- Medical Imaging and Pathology Studies
- Glioma Diagnosis and Treatment
- Meningioma and schwannoma management
- Brain Metastases and Treatment
- Neurological disorders and treatments
- Single-cell and spatial transcriptomics
- Epilepsy research and treatment
- Parkinson's Disease Mechanisms and Treatments
- Soft tissue tumor case studies
- Vascular Malformations Diagnosis and Treatment
- Cerebrospinal fluid and hydrocephalus
- Pharmacological Effects and Toxicity Studies
- Barrier Structure and Function Studies
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- Tendon Structure and Treatment
- Congenital Anomalies and Fetal Surgery
- Autoimmune Neurological Disorders and Treatments
- Transcranial Magnetic Stimulation Studies
- Cerebrovascular and genetic disorders
- Bone Tumor Diagnosis and Treatments
- Sarcoidosis and Beryllium Toxicity Research
- Immune cells in cancer
- Dermatological and COVID-19 studies
- Neuroscience and Neuropharmacology Research
University of California, San Francisco
2023-2024
Broad Center
2023
University of California, Los Angeles
2023
City College of San Francisco
2023
Baby Memorial Hospital
2016
Sree Chitra Thirunal Institute for Medical Sciences and Technology
2012
Christian Medical College & Hospital
1991-2002
Christian Medical College
1991-2001
Sultan Qaboos University
1992-1993
Women's and Children's Hospital
1977
Abstract Meningiomas are the most common primary intracranial tumors. Treatments for patients with meningiomas limited to surgery and radiotherapy, systemic therapies remain ineffective or experimental. Resistance radiotherapy is in high-grade cell types signaling mechanisms that drive meningioma tumorigenesis resistance incompletely understood. Here, we report NOTCH3 drives find perivascular NOTCH3+ stem cells conserved across from humans, dogs, mice. Integrating single-cell transcriptomics...
Thirty consecutive Indian patients with focal or generalised seizures and single, small (less than 10 mm), enhancing lesions on CT scans (SSECTL) were studied. Five (Group A) treated anticonvulsants alone did not have a biopsy. In ten B) guided stereotaxic biopsy of the lesion was carried out in remainder (15-Group C) excision following localisation. all Group B reported as "chronic nonspecific inflammation". seven 15 C showed cysticercus granuloma further five pathology that "parasitic...
Plasticity of primary motor cortex is severely impaired in Parkinson's disease and chronic dopaminergic treatment reported not to rescue it. The effect an acute dose levodopa on cortical plasticity so far variable. In this study, it was hypothesized that would be restored as a long duration response stable responders while those with complications have reduction or loss similar the decay signs. Patients were carefully stratified based their into (n = 17), fluctuating non-dyskinetics 18)...
Non-operative management of splenic trauma is not a widely accepted method treatment. During the period 1964 to 1976, 39 children were treated in Adelaide Children's Hospital for trauma. Twenty-four managed without operation, while 15 by splenectomy. Recently, scan has helped diagnosis and injuries. From our observations, it appears that non-operative treatment proven injury safe ideal initial paediatric age group, when spleen only intraabdominal organ injured, vital signs are stable with...
Meningiomas are the most common primary intracranial tumors
Stiff-man syndrome is a disease of sporadic occurrence. An autoimmune pathogenesis thought to involve the spinal interneuronal circuits, resulting in isolation motor neurons from their inhibitory influence1. Most reported cases have been adults.
Capillary hemangioma is a rare tumor in spinal intradural location. Despite the rarity, early recognition important because of risk hemorrhage. This case report woman who had capillary cauda equina.A 54 -year-old presented with low backache, radiating to left leg for 2 months. She extensor hallucis weakness, sensory impairment L5 dermatome, and mild tenderness lower lumbar spine. Magnetic resonance imaging (MRI) LS spine showed L4/5 tumor, completely occluding canal myelogram, enhancing...
Abstract Background Meningeal solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms that associated with local recurrence and hematogenous metastasis. The cell states spatial transcriptomic architecture underlying the unique clinical behavior of meningeal SFTs unknown. Methods Single-cell (n = 4), 8), bulk RNA sequencing 22) were used to define across histological grades in patient-matched pairs primary/recurrent or intracranial/metastatic samples. Immunofluorescence,...
<p>Supplementary Fig. 2. Single-cell RNA sequencing of dog meningiomas. A, counts across UMAP clusters from meningioma samples analyzed using single-cell sequencing. B, showing shaded by sample origin. C, cell cycle analysis samples. D-E, Heatmapt and feature plots differentially expressed genes Colors as in A. F, Magnetic resonance imaging (MRI, left) or H&E images (right) spontaneous Scale bars, 100µm. See also Supplementary Table 2.</p>
<p>Supplementary Fig. 3. NOTCH3 immunohistochemistry across meningioma WHO grades. expression is predominantly restricted to the perivascular niche in grade 1 meningiomas A, but extends beyond 2 B and 3 C. SF numbers indicate individual meningiomas. defined using histological criteria. Representative of n=10 per grade. Scale bar, 100µm.</p>
<p>Supplementary Fig. 16. NOTCH3 inhibition blocks meningioma xenograft growth without causing toxicity. A, A NOTCH1 negative regulatory region neutralizing antibody (αNRR1), but not αNRR3, causes diarrhea and rash resulting in weight loss mice harboring xenografts. Antibodies were delivered using biweekly IP injection. Student’s <i>t</i> tests. B, The -secretase inhibitor LY-411575 (GSI) daily injection C, attenuates the of CH-175MN (left) or IOMM-Lee (right) xenografts...
<p>Supplementary Fig. 4. WHO grade 1 meningioma immunofluorescence microscopy for NOTCH3 and the mural cell marker SMA. expression is restricted to perivascular niche colocalizes with cells in meningiomas histology. SF numbers indicate individual meningiomas. defined using histological criteria. Representative of n=10 Scale bars, 10µm.</p>
<p>Supplementary Fig. 1. Single-cell RNA sequencing of human meningiomas with loss chromosome 22q. A, counts across UMAP clusters from meningioma samples 22q analyzed using single-cell sequencing. B, showing shaded by sample origin. C, Stacked bar plots the distribution (red) samples. D, and stacked plot cell cycle analysis E-G, Heatmap feature differentially expressed genes Colors as in A. See also Supplementary Table G, Feature expression (SSTR2, PTGDS) or cancer stem-cell markers...
<div>Abstract<p>Meningiomas are the most common primary intracranial tumors. Treatments for patients with meningiomas limited to surgery and radiotherapy, systemic therapies remain ineffective or experimental. Resistance radiotherapy is in high-grade cell types signaling mechanisms that drive meningioma tumorigenesis resistance incompletely understood. Here, we report NOTCH3 drives find perivascular NOTCH3<sup>+</sup> stem cells conserved across from humans, dogs,...
<p>Supplementary Fig. 14. Meningioma invasion of Virchow-Robin spaces. A, H&E low magnification (top) and high (box, bottom) images human meningioma into perivascular fluid-filled cavities surrounding perforating vasculature the brain. Densely cellular is shown at top each image, islands tumor within brain parenchyma (dashed lines, are bottom image. Scale bars, 100µm. B, Low IHC for marker GFAP validates unlabeled cells spaces without direct itself. C, mural cell SMA or SSTR2A...
<p>Supplementary Fig. 12. Single-cell RNA sequencing of human and mouse cells from meningioma xenografts. A, counts across experimental conditions, biological replicates (samples), UMAP clusters CH-157MN xenograft analyzed using single-cell sequencing. B, replicates, phases the cell cycle Colors rows (samples) as in A. C, D, Stacked bar plots showing distribution replicates. E, C.</p>
<p>Supplementary Fig. 6. WHO grade 3 meningioma immunofluorescence microscopy for NOTCH3 and the mural cell marker SMA. is expressed in out of perivascular niche, colocalizes with cells meningiomas histology. SF numbers indicate individual meningiomas. Representative n=10 defined using histological criteria. Scale bars, 10µm.</p>
<p>Supplementary Fig. 7. WHO grade 1 meningioma immunofluorescence microscopy for NOTCH3 and the endothelial cell marker VWF. expression is restricted to perivascular niche adjacent cells in meningiomas with histology. SF numbers indicate individual meningiomas. Representative of n=10 defined using histological criteria. Scale bars, 10µm.</p>
<p>Supplementary Fig. 11. Ptgds is expressed throughout the meninges. Confocal microscopy of whole mount mouse convexity meningeal samples at P7, P30, or P90 after recombination ROSAmT/mG allele using Ptgds-Cre shows PTGDS cells are diffusely perivascular and non-perivascular cells. DAPI marks DNA. Representative n=3 biological replicates per timepoint. Scale bar, 100µm.</p>
<p>Supplementary Fig. 5. WHO grade 2 meningioma immunofluorescence microscopy for NOTCH3 and the mural cell marker SMA. expression is primarily restricted to perivascular niche colocalizes with cells in meningiomas histology. SF numbers indicate individual meningiomas. defined using histological criteria. Representative of n=10 Scale bars, 10µm.</p>