A5110522866- Gene Regulatory Network Analysis
- TGF-β signaling in diseases
- CRISPR and Genetic Engineering
- Cinema and Media Studies
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Law in Society and Culture
- Urological Disorders and Treatments
- Media Studies and Communication
- Developmental Biology and Gene Regulation
- Pediatric Urology and Nephrology Studies
- Renal and related cancers
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Digital Storytelling and Education
- Literacy, Media, and Education
- Art Education and Development
- Participatory Visual Research Methods
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Artistic and Creative Research
- Bioinformatics and Genomic Networks
- Circadian rhythm and melatonin
- Vietnamese History and Culture Studies
Howard Hughes Medical Institute
2013-2024
California Institute of Technology
2016-2024
Fred Hutch Cancer Center
2011-2013
Cancer Research Center
2013
University of California, San Francisco
2007
University of Windsor
1977-1996
A system for programmable export of RNA molecules from living cells would enable both non-destructive monitoring cell dynamics and engineering capable delivering executable programs to other cells. We developed genetically encoded cellular exporters, inspired by viruses, that efficiently package secrete cargo mammalian within protective nanoparticles. Exporting sequencing barcodes enabled population with clonal resolution. Further, incorporating fusogens into the nanoparticles, we...
Development of the metanephric kidney crucially depends on proper interactions between cells and surrounding extracellular matrix. For example, we showed previously that in absence α8β1 integrin,invasion by ureteric bud into mesenchyme is inhibited,resulting renal agenesis. Here present genetic evidence matrix protein nephronectin an essential ligand engagesα8β1 integrin during early development. We show embryos lacking a functional gene frequently display agenesis or hypoplasia, which can...
One goal of aging research is to find drugs that delay the onset age-associated disease. Studies in invertebrates, particularly Caenorhabditis elegans, have uncovered numerous genes involved aging, many conserved mammals. However, which these encode proteins suitable for drug targeting unknown. To investigate this question, we screened a library compounds with known mammalian pharmacology increase C. elegans lifespan. We identified 60 longevity 33 also increased resistance oxidative stress....
In multicellular organisms, secreted ligands selectively activate, or "address," specific target cell populations to control fate decision-making and other processes. Key cell-cell communication pathways use multiple promiscuously interacting receptors, provoking the question of how addressing specificity can emerge from molecular promiscuity. To investigate this issue, we developed a general mathematical modeling framework based on bone morphogenetic protein (BMP) pathway architecture. We...
Natural cell death pathways such as apoptosis and pyroptosis play dual roles: they eliminate harmful cells modulate the immune system by dampening or stimulating inflammation. Synthetic protein circuits capable of triggering specific programs in target could similarly remove while appropriately modulating responses. However, actively influence their modes response to natural signals, making it challenging control modes. Here, we introduce naturally inspired "synpoptosis" that proteolytically...
Artificial neural networks provide a powerful paradigm for nonbiological information processing. To understand whether similar principles could enable computation within living cells, we combined de novo-designed protein heterodimers and engineered viral proteases to implement synthetic circuit that performs winner-take-all network classification. This "perceptein" combines weighted input summation through reversible binding interactions with self-activation mutual inhibition irreversible...
Summary Many targeted therapies indirectly suppress cancer cells by inhibiting oncogenic signaling pathways such as Ras 1–4 . This renders them susceptible to resistance and limits their long-term clinical efficacy 4–10 Engineered protein circuits 11–25 have been envisioned an alternative pharmacological inhibition that directly rewires activity cell death. However, it has remained unclear whether engineered can potently safely treat cancers. Here, we show Ras-targeting accurately...
Abstract Artificial neural networks provide a powerful paradigm for information processing that has transformed diverse fields. Within living cells, genetically encoded synthetic molecular could, in principle, harness principles of computation to classify signals. Here, we combine de novo designed protein heterodimers and engineered viral proteases implement circuit performs winner-take-all network computation. This “perceptein” includes modules compute weighted sums input concentrations...
Abstract In multicellular organisms, secreted ligands selectively activate, or “address,” specific target cell populations to control fate decision-making and other processes. Key cell-cell communication pathways use multiple promiscuously interacting receptors, provoking the question of how addressing specificity can emerge from molecular promiscuity. To investigate this issue, we developed a general mathematical modeling framework based on bone morphogenetic protein (BMP) pathway...
Summary Cell-cell communication systems typically comprise families of ligand and receptor variants that function together in combinations. Pathway activation depends a complex way on which ligands are present what receptors expressed by the signal-receiving cell. To understand combinatorial logic such system, we systematically measured pairwise Bone Morphogenetic Protein (BMP) interactions cells with varying expression. Ligands could be classified into equivalence groups based their profile...
ABSTRACT There are three ways to examine film: making films, watching and talking and/or reading about films. A course currently taught at the University of Windsor, Ontario, aims integrate these approaches
This study briefly outlines the different Canadian, U.S., and Australian experiences with permitting of camera access to court proceedings. A number factors are advanced account for these differences main determinant is identified as a nation’s relative emphasis on values “free press” “fair trial.”
Garth Jowett and James M. Linton, Movies as Mass Communication (Beverly Hills, California: Sage Publications -- $12.50 cloth/$5.95 paper) Motion Picture Institute of Canada, Feature Film Finance Seminar (write Jeanne Ritter, The 250 Merton Street, Toronto, Ontario M4S 181 or order from Boxoffice Magazine, 7950 College Boulevard, P.O. Box 2939, Shawnee Mission, Kansas 66201 $50.00 through the latter source) Lary May, Screening Out Past: Birth Culture Industry (New York: Oxford University...