Abstract Mayaro virus (MAYV) is an emerging mosquito‐borne arbovirus present in Central and South America that causes arthralgia febrile illness. Domestic mosquitoes Aedes aegypti (Diptera: Culicidae) albopictus are potential vectors of MAYV may allow for transmission to humans urban settings. The paper assesses susceptibility infection, disseminated infection Florida Ae. MAYV. Oral was significantly higher (85–100%) than (67–82%). Viral dissemination the haemocoel rapid co‐occurred with...

Chikungunya virus (CHIKV) is a vector-borne alphavirus transmitted by the bites of mosquitoes, specifically infected, female mosquitoes invasive Aedes species. In nature, CHIKV can be maintained vertical transmission, phenomenon that relates to transfer from infected parent their offspring within ovary or during oviposition. present study, we conducted laboratory experiments determine transmission with Ae. albopictus populations Brazil and Florida. Parental females were orally emergent Asian...

Between 2014 and 2016 more than 3,800 imported human cases of chikungunya fever in Florida highlight the high risk for local transmission. To examine potential sustained transmission virus (CHIKV) we tested whether populations Aedes aegypti albopictus show differences susceptibility to infection two emergent lineages CHIKV, Indian Ocean (IOC) Asian genotypes (AC) laboratory experiments. All examined Ae. mosquitoes displayed infection, rapid viral dissemination into hemocoel, both CHIKV. had...

Wolbachia transinfections established in key mosquito vectors, including Aedes aegypti are typically associated with pathogen blocking-reduced susceptibility to infection pathogens and reduced likelihood those transmitted new hosts. Host-symbiont-virus interactions less well understood mosquitoes like Culex quinquefasciatus, which naturally harbor Wolbachia, blocking observed some populations but not others, potentially due innate differences their load. In nature, larvae often subject...

Climate strongly influences the geographic distribution and timing of mosquito-borne disease outbreaks. Environmental temperature affects phenotypic traits mosquitoes including vector competence for arboviruses mediated by changes in infection, extrinsic incubation period rates transmission. Most experiments, however, are done at constant temperatures. In nature, more likely to experience daily fluctuations temperature. Here we compare disseminated infection (leg infection) saliva Aedes...

Chikungunya virus is a vector-borne alphavirus transmitted by the bites of infected female Ae. aegypti and albopictus. In Brazil between 2014 2016 almost 320 thousand autochthonous human cases were reported in Florida numerous imported CHIKV viremic (> 3,800) demonstrate potential high risk to establishment local transmission. present study, we carried out series experiments determine viral dissemination transmission rates different Brazilian populations albopictus at 2, 5, 13 days...

The environmental conditions experienced by parents can influence offspring phenotype along with the offspring. These parental effects are clear in organisms that display care and less other organisms. Here, we consider of larval nutrition on development time, survivorship infection dengue virus Aedes aegypti, mosquito vector dengue, chikungunya, yellow fever Zika. Parents were raised either high or low detritus inputs subsequent being divided into two groups, one receiving nutrients low....

In recent years, there has been a rise in the emergence of arboviruses public health importance, including Zika, chikungunya, dengue, and yellow fever viruses. Insecticide-based mosquito control primary method for mitigating transmission arboviruses. The consequences application insecticides include both lethal sublethal effects, associated development insecticide resistance. However, little is known about influence on arboviral transmission. Mosquitoes with phenotypes that exhibit...

Zika virus (ZIKV) is an emerging mosquito-borne pathogen that can cause global public health threats. In the absence of effective antiviral medications, prevention measures rely largely on reducing number adult mosquito vectors by targeting juvenile stages. Despite importance control in population size, a full understanding effects these determining phenotypic traits and mosquito-arbovirus interactions poorly understood. Pyriproxyfen hormone analog primarily blocks emergence, but does not...

Vector control has been an essential strategy in Brazil to manage vector-borne diseases, and the use of insecticides plays important role this effort. Pyriproxyfen (PPF) become a common insect growth regulator used juvenile stages mosquitoes by disturbing their development. This study assesses susceptibility resistance status Brazilian Ae. aegypti populations that previously showed low levels PPF. Eggs were collected from six cities located northeast states Ceará (Quixadá, Icó, Juazeiro do...

Mayaro virus is a mosquito-borne Alphavirus endemic to forests of tropical South America with sylvatic cycle involving non-human primates and Haemagogus mosquitoes. Human infection causes febrile illness long-lasting arthralgia cases are often associated exposure forest habitats. movement between habitats urban settings may allow for imported subsequent local transmission by domestic mosquito Aedes aegypti. The relative importance Ae. aegypti as vector depend on the pathogenic effects...

Vector competence refers to the ability of a vector acquire, maintain, and transmit pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become standard for approximating arbovirus transmission. However, this method is time-consuming labor-intensive. Here we compare tube an alternative high-throughput detection collection on paper cards saturated with honey, (FTA card) without (filter paper) reagents preservation nucleic acid Aedes aegypti albopictus mosquitoes infected...

Dengue virus (DENV) is a highly prevalent vector-borne that causes life-threatening illnesses to humans worldwide. The development of tool control vector populations has the potential reduce burden DENV. Toxic sugar bait (TSB) provides form takes advantage sugar-feeding behavior adult mosquitoes. However, studies on effect ingestion toxins in TSB competence and vectorial capacity for viruses are lacking. This study evaluated DENV serotype-1 Aedes albopictus at 7 14 days post-ingestion...

Arthropod-borne viruses are major causes of human and animal disease, especially in endemic low- middle-income countries. Mosquito-borne pathogen surveillance is essential for risk assessment vector control responses. Sentinel chicken serosurveillance (antibody testing) mosquito pool screening (by RT-qPCR or virus isolation) currently used to monitor arbovirus transmission, however substantial time lags seroconversion and/or laborious identification RNA extraction steps sacrifice their early...

Abstract West Nile virus (WNV) is the leading cause of mosquito-borne disease in United States. Landscapes provide habitats and resources for WNV mosquito vectors avian amplification hosts, landscape composition configuration can affect transmission opportunities. We examined correlations with Florida, USA across state within five regions to understand potential hazard. expected higher percentages residential or medium-developed landscapes more fragmented would be positively correlated WNV,...

Abstract West Nile virus (WNV) is the leading cause of mosquito-borne disease in United States. Landscapes provide habitats and resources for WNV mosquito vectors avian amplification hosts, landscape composition configuration can affect transmission opportunities. We examined correlations with Florida, USA across state within five regions to understand potential hazard. expected higher percentages residential or medium-developed landscapes more fragmented would be positively correlated WNV,...

How landscape composition and configuration impact the distribution of multi-vector multi-host mosquito vector-borne disease systems, such as West Nile virus (WNV), remains challenging because complex habitat resource requirements by hosts vectors that affect transmission opportunities. We examined correlations between 2018 WNV sentinel chicken seroconversion in Florida, USA across state within five National Oceanic Atmospheric Administration (NOAA) bioclimatic regions to understand strength...

Competition between mosquito species during the larval phase is a well-established mechanism structuring container communities, with invasive often outperforming natives. We assessed competitive outcome 2 that occur on island of Puerto Rico, historic Aedes aegypti (L.) and endemic mediovittatus (Coquillett) (Diptera: Culicidae). Trials were conducted under intra- interspecific densities crossed amounts 4 different leaf types serving as detrital resources for developing larvae; representative...

Recently reported "displaceable probe" loop amplification (DP-LAMP) architecture has shown to amplify viral RNA from SARS-CoV-2 with little sample processing. The allows signals indicating the presence of target nucleic acids be spatially separated, and independent in sequence, complicated concatemer that LAMP processes create as part their process. This makes DP-LAMP an attractive molecular strategy integrate trap sampling innovations detect arboviruses carried by mosquitoes field. These...

Control of mosquito vector populations is primarily intended to reduce the transmission pathogens they transmit. Use chemical controls, such as larvicides, can have unforeseen consequences on adult traits if not applied properly. The under application larvicides are little studied, specifically impacts pathogen infection and by vectors that survive exposure larvicides. We compared susceptibility Aedes aegypti (L.) for dengue virus, serotype 1 (DENV-1) previously exposed larvae an LC50...

Zika virus (ZIKV) infection has caused devastating consequences in Brazil as infections were associated with neurological complications neonates. Aedes aegypti is the primary vector of ZIKV, and evolution insecticide resistance (IR) this species can compromise control efforts. Although relative levels phenotypic IR mosquitoes change considerably over time, its influence on competence for arboviruses unclear. Pyriproxyfen (PPF)-resistant populations Ae. collected from five municipalities...