A5022711435- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Insect Pest Control Strategies
- Dengue and Mosquito Control Research
- Biosensors and Analytical Detection
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect and Pesticide Research
- Evolution and Genetic Dynamics
- Vector-Borne Animal Diseases
- Ecology and Vegetation Dynamics Studies
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Insect Utilization and Effects
- Animal Disease Management and Epidemiology
- Insect-Plant Interactions and Control
- Species Distribution and Climate Change
- Animal Behavior and Reproduction
- Studies on Chitinases and Chitosanases
- Zoonotic diseases and public health
- SARS-CoV-2 detection and testing
- Virology and Viral Diseases
University of Florida
2016-2025
Florida Medical Entomology Laboratory
2016-2025
Orthopaedic Center of Vero Beach
2019
Google (United States)
2018
Yale University
2008-2013
Illinois Archaeological Survey
2011
University of Illinois Urbana-Champaign
2009-2011
Illinois Department of Natural Resources
2010
University of Illinois System
2009
Illinois State University
2000-2001
We investigated how temperature and precipitation regime encountered over the life cycle of Aedes albopictus (Skuse) affects populations. Caged populations A. were maintained at 22, 26, 30°C. Cages equipped with containers that served as sites for oviposition larval development. All cages assigned to one three simulated regimes: (1) low fluctuation - water within was allowed evaporate 90% its maximum before being refilled, (2) high 25% (3) drying complete container dryness refilled. Greater...
Dengue, the most important human arboviral disease, is transmitted primarily by Aedes aegypti and, to a lesser extent, albopictus . The current distributions of these invasive species overlap and are affected interspecific larval competition in their container habitats. Here we report that also enhances dengue infection dissemination rates one two vector species. We determined effects on adult A. , comparing susceptibility with Southeast Asian strain dengue-2 virus. High levels intra- or...
Both density-mediated and trait-mediated indirect biotic interactions may be important in structuring communities. Indirect many study systems remain unexplored; part, because they are often difficult to detect, instances, have been identified empirically only when unexpected results arise. effects induced by competition particularly among organisms with complex life cycles, wherein competitive experienced one stage influence species or more subsequent stages. We determined whether...
Temperature is one of the most important environmental factors affecting biological processes mosquitoes, including their interactions with viruses. In these studies, we show independent effects rearing temperature on immature aquatic stages and holding adult terrestrial stage in terms alterations survival progression dengue-1 virus infection Asian tiger mosquito Aedes (Stegomyia) albopictus. Our studies that was determined by adult-holding temperature, regardless conditions stages....
The size of arthropod vectors may affect their ability to transmit pathogens. Here we test the hypothesis that body alters susceptibility Aedes aegypti and albopictus mosquitoes dengue virus (DENV) infection subsequent dissemination throughout mosquito. After feeding on blood containing known quantities virus, smaller-sized females were significantly more likely become infected disseminate than larger individuals. effects stronger for Ae. independent rearing conditions. was susceptible DENV...
Zika, dengue, and chikungunya are three mosquito-borne viruses having overlapping transmission vectors. They cause diseases similar symptoms in human patients, but requiring different immediate management steps. Therefore, rapid (< one hour) discrimination of these patient samples trapped mosquitoes is needed. The need for speed precludes any assay that requires complex up-front sample preparation, such as extraction nucleic acids from the sample. Also precluded robust point-of-sampling...
Summary Objective To evaluate how stress at the larval stage alters adult mosquito performance and susceptibility to viral infection. Methods We used a model system consisting of Sindbis virus (SINV) yellow fever Aedes aegypti. Larvae were either reared under optimal conditions (control) or exposed one four types stressors; suboptimal nutrients, starvation, elevated temperature, low dose insecticide malathion females fed SINV infectious blood meal. Differential expressions stress,...
We investigated how constant temperatures of 22, 24, and 26°C experienced across the full life cycle affected dynamics caged populations Aedes albopictus (Skuse). All cages were equipped with plastic beakers that served as sites for oviposition larval development. measured per capita daily mortality emergence rates adults size adult females, estimated intrinsic rate increase (r) asymptotic density (K) each population. Populations at had greater lower densities than 22 24°C. high initially...
Abstract Mayaro virus (MAYV) is an emerging mosquito‐borne arbovirus present in Central and South America that causes arthralgia febrile illness. Domestic mosquitoes Aedes aegypti (Diptera: Culicidae) albopictus are potential vectors of MAYV may allow for transmission to humans urban settings. The paper assesses susceptibility infection, disseminated infection Florida Ae. MAYV. Oral was significantly higher (85–100%) than (67–82%). Viral dissemination the haemocoel rapid co‐occurred with...
Recent reports of Zika virus (ZIKV) isolates from Culex species mosquitoes have resulted in concern regarding a lack knowledge on the number competent vector for ZIKV transmission new world. Although observations field demonstrated that isolation can be made mosquitoes, detection these is not proof their involvement cycle. Detection may due to recent feeding viremic vertebrate, and indicative replication mosquito. In this study, susceptibility recently colonized was investigated. The results...
Chikungunya virus (CHIKV) is a vector-borne alphavirus transmitted by the bites of mosquitoes, specifically infected, female mosquitoes invasive Aedes species. In nature, CHIKV can be maintained vertical transmission, phenomenon that relates to transfer from infected parent their offspring within ovary or during oviposition. present study, we conducted laboratory experiments determine transmission with Ae. albopictus populations Brazil and Florida. Parental females were orally emergent Asian...
Terrestrial invertebrate carcasses are an important resource for insects developing in pitcher plants. However, little is known of the role these other containers, which also receive leaf fall and stemflow inputs. This experiment investigated effects accumulated as a two competing mosquitoes, Aedes albopictus (Skuse) aegypti (L.), whether either species differentially benefited from carcasses, if such benefit affected interspecific competition. First, we measured accumulation standard...
Terrestrial invertebrate carcasses are an important resource for insects developing in pitcher plants. However, little is known of the role these other containers, which also receive leaf fall and stemflow inputs. This experiment investigated effects accumulated as a two competing mosquitoes, Aedes albopictus (Skuse) aegypti (L.), whether either species differentially benefited from carcasses, if such benefit affected interspecific competition. First, we measured accumulation standard...
Temperature is a key factor influencing mosquito growth and development also known to affect insecticide efficacy. We evaluated the effects of larval rearing temperature exposure insecticides on adult fitness competence for arboviral infection using Sindbis virus (SINV). exposed newly hatched larvae Aedes aegypti an environmentally realistic level malathion at 20°C 30°C allowed resulting adults feed SINV-infected blood meal. Exposure significantly reduced survival adulthood. Statistically...
Following the 2009 and 2010 dengue-1 (DENV-1) outbreaks in Key West, FL, we used Florida Aedes aegypti (L.) mosquitoes DENV-1 isolated from West to test hypothesis that if genome sequences are similar, then vertical transmission of infected Ae. female their eggs could have served as an interepidemic reservoir between outbreaks. We also investigated ability albopictus (Skuse) vertically transmit DENV-1. In addition, determined rates infection dissemination these mosquito species for effect on...
The chronic effects of exposure Culex restuans (Theobald) and Aedes albopictus (Skuse) (Diptera: Culicidae) to low concentrations malathion were examined by exposing larvae the two species four doses at 20, 25, 30 degrees C maintaining resulting adults 25 C. For both species, a significant temperature interaction on survival was found. Greater temperatures highest dosage had significantly lower survivorship than in other treatments, but this effect not observed 20 These results suggest that...
Constant environments are often assumed to favor the evolution of specialization whereas exposure changing may generalists. Here we explored phenotypic and molecular changes associated with evolving an RNA virus in constant versus fluctuating temperature environments. We used vesicular stomatitis (VSV) determine whether selection at a entails performance trade-off unselected temperature, populations evolve be generalists when selected deterministically environments, under stochastically...
Ornithophilic Culex species are considered the primary amplification vectors of West Nile virus (WNV) in bird hosts as well responsible for epidemic transmission. coronator was first collected from Okaloosa, Santa Rosa, Walton, and Washington Counties Florida 2005 has since spread throughout state. The vector competence Cx. WNV, known to be infected nature, not been assessed. Without this knowledge, we unable assess species' potential an enzootic WNV Florida. In current study, investigate...
Between 2014 and 2016 more than 3,800 imported human cases of chikungunya fever in Florida highlight the high risk for local transmission. To examine potential sustained transmission virus (CHIKV) we tested whether populations Aedes aegypti albopictus show differences susceptibility to infection two emergent lineages CHIKV, Indian Ocean (IOC) Asian genotypes (AC) laboratory experiments. All examined Ae. mosquitoes displayed infection, rapid viral dissemination into hemocoel, both CHIKV. had...
Journal Article Vector Competence of Aedes aegypti and albopictus (Diptera: Culicidae) for Dengue Virus in the Florida Keys Get access Stephanie L. Richards, Richards 1 1Corresponding author: East Carolina University, College Health Human Performance, Department Education Promotion, Environmental Sciences Program, 3403 Carol Belk Building, Greenville, NC 27858 (e-mail: richardss@ecu.edu). Search other works by this author on: Oxford Academic PubMed Google Scholar Sheri Anderson, Anderson...
Chromosomal inversions play a fundamental role in evolution and have been shown to regulate epidemiologically important traits malaria mosquitoes. However, they never characterized Aedes aegypti, the major vector of arboviruses, because poor structure its polytene chromosomes. In this study, we applied Hi-C proximity ligation approach identify chromosomal 25 strains Ae. acquired from worldwide distribution, as well one strain mascarensis. The study identified 21 multi-megabase with uneven...
<title>Abstract</title> 1. Vectorial capacity (VC) is a powerful tool for estimating species’ importance in the transmission of vector-borne pathogens and predicting impacts specific control measures on those pathogens. However, VC rarely estimated zoonotic multi-vector, multi-host disease (VBD) systems, due to inherent difficulties measuring several parameters, especially biting rate daily probability survival. 2. We present Vector Potential Index (VPI), novel metric evaluating comparing...
Understanding how evolution promotes pathogen emergence would aid disease management, and prediction of future host shifts. Increased infectiousness different hosts may occur through direct selection, or fortuitously via indirect selection. However, it is unclear which type selection tends to produce breadth promoting emergence. We predicted that for should foster by causing higher population growth on new hosts, lower among-population variance in across hosts. tested the predictions using...
Journal Article Can Pesticides and Larval Competition Alter Susceptibility of Aedes Mosquitoes (Diptera: Culicidae) to Arbovirus Infection? Get access Ephantus J. Muturi, Muturi 2 1Illinois Natural History Survey, University Illinois, 1816 S. Oak Street, Champaign, IL 61820. 2Corresponding author: 61820 (e-mail: ephajumu@yahoo.com). Search for other works by this author on: Oxford Academic PubMed Google Scholar Katie Costanzo, Costanzo 3Department Biology, Canisius College, 2001 Main...
Mosquito larvae face numerous biotic and abiotic challenges that affect their development survivorship, as well adult fitness. We conducted two experiments under semi‐natural conditions to evaluate the effects of intraspecific competition, nutrient limitation sub‐lethal doses malathion on individual life history traits in Culex pipiens (Diptera: Culicidae). In first experiment, Cx. were reared at different densities exposed malathion. second low or high larval nutrients, subsequent adults...