A5101472602- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Intracranial Aneurysms: Treatment and Complications
- Fungal Biology and Applications
- Vascular Anomalies and Treatments
- Neonatal and fetal brain pathology
- MicroRNA in disease regulation
- Ion Transport and Channel Regulation
- Healthcare and Venom Research
- Liver Disease and Transplantation
- Advanced Glycation End Products research
- 14-3-3 protein interactions
- Moyamoya disease diagnosis and treatment
- Cancer-related gene regulation
- Beetle Biology and Toxicology Studies
- MRI in cancer diagnosis
- Phagocytosis and Immune Regulation
- Chromatography in Natural Products
- Neuroinflammation and Neurodegeneration Mechanisms
Xinxiang Medical University
2009-2024
Chinese Center For Disease Control and Prevention
2023
Wenzhou Medical University
2022
First Affiliated Hospital of Wenzhou Medical University
2022
Shanghai Changzheng Hospital
2020
Songjiang District Central Hospital
2007-2012
Abstract Background Collagens are the most abundant proteins in extra cellular matrix and important components of tumor microenvironment. Recent studies have showed that aberrant expression collagens can influence cell behaviors. However, their roles hepatocellular carcinoma (HCC) poorly understood. Methods In this study, we screened all 44 collagen members HCC using whole transcriptome sequencing data from public datasets, type IV alpha1 chain (COL4A1) was identified as significantly...
The aim of this study was to investigate the effects progesterone (PROG) on blood-brain barrier (BBB) permeability, cerebral edema and expression matrix metalloproteinase-9 (MMP-9) aquaporin-4 (AQP-4) in neonatal rats with hypoxic-ischemic brain damage (HIBD) explore mechanism its neuroprotective effect. Sixty 7-day-old Wistar were divided into sham surgery, hypoxic ischemia (HI) drug prophylaxis groups. HIBD animal models established. All animals sacrificed after 24 h. BBB assessed using...
Background: Colon cancer is one of the most common malignant tumors, and B cell Translocation Gene (BTG)1 involved in occurrence development colon cancer, however, underlying molecular mechanism remains unclear. Objective: In this study, we investigated expression BTG1 protein its association with clinicopathology prognosis. Methods: The tumor specimens from 59 patients who had undergone radical colectomy were selected as observation group. Para-carcinoma tissues same control expressions...
Recent studies found that progesterone (PROG) has a noticeable preventive effect on brain injuries. However, the underlying mechanisms of these effects, particularly hypoxic ischemic damage (HIBD), remain unclear. This study observed influence PROG expression cycloxygenase-2 (COX-2) and interleukin-1β (IL-1β) in HIBD neonatal rats, explored corresponding molecular neuroprotective PROG. Sixty 7-day-old Wistar rats were divided into sham operation (control), ischemia (HI), drug prophylaxis...
Background: This study aimed to investigate the geographical distribution of dietary patterns and their association with T2DM among Chinese adults aged 45 years above. Methods: Data was from China Adults Chronic Diseases Nutrition Surveillance (2015). Dietary intake for each participant determined through a combination 3-day 24-h recall interviews food frequency questionnaires. Principal component analysis used extract spatial employed geographic them. diagnosed using criteria ADA 2018,...
Abstract Background Postoperative delirium (POD) is a common complication after cardiac surgery (CS), with symptoms like attention disorders and even delays patients' recovery. Aims To evaluate the impact of preoperative on‐site visits in care unit (CCU) on POD CS. Study Design Patients admitted to CCU extracorporeal CS were included visiting or non‐visiting group according whether they week. The received visit from nurse‐led multidisciplinary team (including nurses physicians) 1 week before...
The purpose of the current study was to report a simple and practical method prepare high molecular weight (mw) DNA ladders. involves 1,000-4,000-base pairs (bp) fragments being amplified by polymerase chain reaction (PCR), using λ as template. constructed plasmids are digested restriction endonucleases produce 5-, 6-, 8- 10-kb fragments, followed purification precipitation with ethanol, mixed proportionally. 1,000-4,000-bp were successfully generated PCR obtained through digestion plasmids....
The aim of the present study was to investigate effect characteristic elements matrix attachment region (MAR) on transgene expression. Human β‑globin MAR obtained by PCR amplification. A splicing fragment containing all artificially synthesized and then cloned into eukaryotic expression vector. Following digestion sequence identification, we transfected Chinese hamster ovary (CHO) cells with two vectors, screened for transformation stable cells. level analyzed ELISA, copy numbers CAT gene...
Background: Esophageal carcinoma is one of the common malignant tumors in digestive tract. BECLIN-1 a key gene that regulates autophagy, and its abnormal expression may be related with many human tumors. However, mechanism esophageal remains unknown. Objective: In this study, we explored effect overexpression on tumor growth mice mechanism. Methods: Recombined lentiviral vector containing was used to transfect Eca109 cells establish stable cell line. qRT-PCR detect mRNA level transfected...
The aim of this study is to investigate the effect chimeric intron in different directions on expression nerve growth factor (NGF) recombinant Chinese hamster ovary (CHO) cells. that contained splice sequence first human β-globin and immunoglobulin heavy chain variable region was used. NGF gene cloned into vectors containing forward or reverse direction, followed by transfecting CHO cells, screened under G418 produce stable transfected Fluorescence quantitative PCR, ELISA, Western blotting...
Abstract Background: Collagens are the most abundant proteins in extra cellular matrix and important components of tumor microenvironment. Recent studies have showed that aberrant expression collagens can influence cell behaviors. However, their roles hepatocellular carcinoma (HCC) poorly understood. Methods: In this study, we screened all 44 collagen members HCC using whole transcriptome sequencing data from public datasets, type IV alpha1 chain (COL4A1) was identified as significantly...