A5047178433- HIV Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Surgical Treatments
- Angiogenesis and VEGF in Cancer
- Tryptophan and brain disorders
- Gut microbiota and health
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Peptidase Inhibition and Analysis
- Esophageal Cancer Research and Treatment
- Epigenetics and DNA Methylation
- TGF-β signaling in diseases
- Hippo pathway signaling and YAP/TAZ
- HIV/AIDS drug development and treatment
- Colorectal Cancer Screening and Detection
- Cancer Immunotherapy and Biomarkers
- Protease and Inhibitor Mechanisms
- Renin-Angiotensin System Studies
- Herpesvirus Infections and Treatments
- Biochemical and Molecular Research
- Gastrointestinal Tumor Research and Treatment
- Electrochemical Analysis and Applications
- NF-κB Signaling Pathways
Kumamoto University
2011-2023
Shizuoka Cancer Center
2010
Tokyo Institute of Psychiatry
2003
RIKEN Center for Brain Science
2003
Abstract Background Impaired HIV-1 Gag, Pol, and Env function has been described in elite controllers (EC) who spontaneously suppress plasma viremia to < 50 RNA copies/mL; however, activity of the accessory protein Nef remains incompletely characterized. We examined ability 91 clones, isolated from 45 EC 46 chronic progressors (CP), down-regulate HLA class I CD4, up-regulate II invariant chain (CD74), enhance viral infectivity, stimulate replication PBMC. Results In general, clones were...
Abstract Glucose transporter 1 ( GLUT 1) expression is a prognostic marker for esophageal squamous cell carcinoma ESCC ). Recent work on and development of specific inhibitors supports the feasibility inhibition as treatment various cancers. The anti–proliferative effects 1‐specific small interfering RNA (si ) inhibitor were evaluated in lines. Expression pro–proliferative signaling effector molecules was examined by western blotting quantitative RT ‐ PCR . pretreatment clinical biopsy...
Abstract Fusobacterium nucleatum has been detected in 8%‐13% of human colorectal cancer, and shown to inhibit immune responses against primary tumors animal models. Thus, we hypothesized that the presence F . might be associated with reduced T cell density cancer liver metastases (CRLM). We quantified DNA 181 CRLM specimens using quantitative PCR assay. The densities CD8 + cells, CD33 cells (marker for myeloid‐derived suppressor [MDSCs]), CD163 tumor‐associated macrophages [TAMs]) tissue...
Chronic inflammation has a crucial role in cancer development and the progression of various tumors, including pancreatic ductal adenocarcinoma ( PDAC ). The arachidonate cascade is major inflammatory pathway that produces several metabolites, such as prostaglandin E2. enzyme 15‐hydroxyprostaglandin dehydrogenase (15‐ PGDH ) degrades frequently decreased types cancer; however, molecular mechanisms 15‐ suppression are unclear. current study was carried out to elucidate clinical significance ....
Peritoneal dissemination is the most common metastatic pattern in advanced gastric cancer (GC) and has a very poor prognosis. However, its molecular mechanism not been elucidated. Our study investigated genes associated with peritoneal of GC. We performed combined expression analysis GC cell lines identified Procollagen-lysine, 2-oxoglutarate 5-dioxygenase2 (PLOD2) as potential regulator dissemination. PLOD2 regulated by hypoxia-inducible factor-1 (HIF-1) mediates extracellular matrix...
HIV-1 Nef downregulates the viral entry receptor CD4 as well coreceptors CCR5 and CXCR4 from surface of HIV-infected cells, this leads to promotion replication through superinfection resistance other mechanisms. sequence motifs that modulate these functions have been identified via in vitro mutagenesis with laboratory strains. However, it remains unclear whether same contribute activity patient-derived sequences may differ isolated at different infection stages and/or patients disease...
Abstract Indoleamine 2, 3‐dioxygenase 1 ( IDO 1) is a primary enzyme that generates immunosuppressive metabolites. It plays major role in tumor immunology and potential immune‐based therapeutic target. We have reported protein expression was associated with an unfavorable clinical outcome esophageal cancer. Recently, it has been regulated by methylation of the promoter. Thus, aim this study to examine relationship between expression, promoter methylation, clinicopathological features first...
Pancreatic ductal adenocarcinoma (PDAC) is characterized by abundant stroma and cancer-associated fibroblasts (CAFs) provide a favorable tumor microenvironment. Smad4 known as suppressor in several types of cancers including PDAC, loss triggers accelerated cell invasiveness metastatic potential. The thrombospondin-1 (TSP-1) can act major activator latent transforming growth factor-β (TGF-β) vivo. However, the roles TSP-1 mediator TGF-β signal activation during PDAC progression have not yet...
Microsatellite instability (MSI) is categorized by mutation frequency: high MSI (MSI-H), low (MSI-L) and microsatellite stable (MSS). MSI-H tumors have a distinct immunogenic phenotype, with immunotherapies using checkpoint inhibitors already approved for the treatment of gastroesophageal adenocarcinoma (GEA); this not observed MSI-L or MSS. Here, we tested hypothesis that are also phenotype potentially immunogenic. MSI-PCR assays (BAT25, BAT26, BAT40, D2S123, D5S346 D17S250) were performed...
Background Elite controllers (EC) are HIV-1 infected persons who can spontaneously control viremia to extremely low levels. Because it is known that nef required for HIV1 replication and pathogenesis in vivo, we wish clarify how plays a role acquiring EC phenotype. Materials methods Viral RNAs were extracted from plasma samples of 45 ECs 48 chronic progressors (CP) used amplification by RT-PCR. The amplified products cloned into plasmid subsequently sequenced. A representative clone was...
We previously demonstrated by X-ray crystallography and electrospray mass spectrometry that D52E mutant hen lysozyme formed a covalent enzyme–substrate adduct on reaction with N-acetylglucosamine oligomer. This observation indicates may acquire catalytic pathway via adduct. To explain this pathway, the formation hydrolysis reactions of were investigated. Kinetic analysis indicated step was rate-limiting step, 60-fold slower than reaction. In reaction, pH dependence bell-shaped, which...
Materials and methods Plasma HIV RNA-derived Nef clones (one per patient) were isolated from 45 chronically-infected subjects inserted into the pNL43 proviral vector. Recombinant viruses prepared used to infect HLA-ldeficient cell line 721.221 ectopically expressing either HLA-A*24 or HLA-B*35. Following infection, cell-surface HLA-I expression of virus-infected cells was evaluated by flow cytometry using a pan specific antibody [2,3].
Abstract Background: Pancreatic cancer is a serious life-threatening disease due to its highly invasive and metastatic potential. Hyperglycemia well-known initial symptom in patients with pancreatic ductal adenocarcinoma (PDAC). Metabolic reprogramming cancer, described as the Warburg effect, can induce epithelial-mesenchymal transition (EMT). In this study, we elucidate biological clinical impact of hyperglycemia PDAC progression. Methods: Biological on malignant behavior was examined by...