A5045396494- Oral microbiology and periodontitis research
- Gut microbiota and health
- Computational Drug Discovery Methods
- Dental Health and Care Utilization
- Brucella: diagnosis, epidemiology, treatment
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Biochemical Analysis and Sensing Techniques
- Vibrio bacteria research studies
- Marine Bivalve and Aquaculture Studies
- Machine Learning in Materials Science
- Animal Diversity and Health Studies
- Burkholderia infections and melioidosis
- Escherichia coli research studies
- Data-Driven Disease Surveillance
- Plant Pathogenic Bacteria Studies
- Pulmonary Hypertension Research and Treatments
- Pineapple and bromelain studies
- Transgenic Plants and Applications
- Biochemical and Structural Characterization
- Advanced Chemical Sensor Technologies
- Infection Control and Ventilation
University of Manitoba
2021-2025
Children's Hospital Research Institute of Manitoba
2021-2024
Kuwait University
2016-2023
Indian Institute of Technology Bombay
2013
Brucellosis is a zoonotic disease of major concern in Kuwait and the Middle East. Human brucellosis can be caused by several Brucella species with varying degree pathogenesis, relapses are common after apparently successful therapy. The classical biochemical methods for identification time-consuming, cumbersome, provide information limited to level only. In contrast, molecular rapid differentiation at intra-species level. this study, four [16S rRNA gene sequencing, real-time PCR,...
Early childhood caries (ECC) is a multifactorial disease mainly caused by the oral microbiome; however, it also influenced host genetics and environmental factors. The combinatorial analysis of these multiple factors influencing ECC susceptibility requires further research. This study investigated interplay between genetic variants in taste-related genes microbiome ECC, targeting taste because their role preference potential interactions with fungi bacteria. Using case-control design...
The human oral cavity harbors one of the most diverse microbial communities with different microenvironments allowing colonization unique species. This study aimed to determine which two commonly used sampling sites (dental plaque vs. swab) would provide a better prediction model for caries-free severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, total 80 children (40 S-ECC 40 caries-free) < 72 months age were...
The microorganisms at the workplace contribute towards a large portion of biodiversity person encounters in his or her life. Health care professionals are often risk due to their frontline nature work. Competition and cooperation between nasal bacterial communities individuals working health setting have been shown mediate pathogenic microbes. Therefore, we investigated community 47 healthy clinical research laboratory Kuwait. taxonomic profiling core microbiome analysis identified three...
Chemical-genetic interaction profiling is a genetic approach that quantifies the susceptibility of set mutants depleted in specific gene product(s) to chemical compounds. With recent advances artificial intelligence, chemical-genetic profiles (CGIPs) can be leveraged predict mechanism action This achieved by using machine learning, where data from CGIP fed into learning platform along with descriptors develop chemogenetically trained model. As small molecules considered non-structural data,...
Human brucellosis is a neglected and underrecognized infection of widespread geographic distribution. Brucellosis present on all inhabited continents endemic in many areas the world, including Kuwait Middle East. Here, we draft genome assemblies five Brucella melitensis strains isolated from patients Kuwait.
Early childhood caries (ECC) is a multifactorial disease with microbiome playing significant role in progression. Understanding changes at the level ECC required to develop diagnostic and preventive strategies. In our study, we combined data from small independent cohorts compare composition using unified pipeline applied batch correction avoid pitfalls of effects. Our meta-analysis identified common biomarker species between different studies. We best machine learning method for...
Persistent pulmonary hypertension of the newborn (PPHN) is a hypoxic disorder vascular relaxation, mediated in part by adenylyl cyclase (AC). Neonatal arteries (PA) express mainly AC6 isoform, followed AC3, 7 and 9. expression upregulated hypoxia. We reported AC enzyme inhibition due to S-nitrosylation PPHN PA, PA myocytes exposed hypothesize that hypoxia promotes cysteine thiol nitrosylation AC6, impairing cAMP production. HEK293T cells stably expressing isoforms (AC3, 5, 6, 7, 9), or...
Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, oral health. In this cross-sectional study, we analyze interkingdom dynamics in the dental plaque microbiome its association with variables. We use 16S rRNA ITS1 amplicon sequencing on samples collected from preschool children questionnaire data to examine social determinants of The results indicate a significant enrichment Streptococcus mutans Candida dubliniensis ECC samples, contrast...
Acinetobacter baumannii is an important opportunistic pathogen in global health care settings. Its dissemination and multidrug resistance pose issue with treatment outbreak control. Here, we present draft genome assemblies of six multidrug-resistant clinical strains A. isolated from patients admitted to one two major hospitals Kuwait.
Background: Human brucellosis is present on all inhabited continents with high prevalence in many areas of the world, including Kuwait and Middle East. To implement proper control measures, identification characterization Brucella species genotypes are required through a reliable rapid subtyping method. In previous studies, whole-genome sequencing (WGS) has shown its potential as an epidemiological typing tool. Using WGS data, this study aimed to identify species, phage sequences, putative...
SUMMARY Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, oral health. In this cross-sectional study, we analyzed interkingdom dynamics in the dental plaque microbiome its association with variables. The samples collected from preschool children underwent 16S rRNA ITS1 gene sequencing. questionnaire data were for social determinants of results indicated a significant enrichment Streptococcus mutans Candida dubliniensis ECC samples,...
Objective: The identification of Brucella genotypes is essential for epidemiological studies. whole genome sequencing emerging as a novel tool genetic characterization infectious microbes. aim this study was to genotype B. melitensis isolates from Kuwait using and variant analysis the sequence data. Methods: DNA were purified 15 heat-inactivated used prepare libraries employing Nextera XT Sample Preparation Kit (Illumina San Diego, CA, USA) sequenced on MiSeq (Illumina). files aligned three...
Calanoid copepod populations are being severely affected due to the effects of ocean acidification (OA) and warming (OW). These marine organisms most abundant primary consumers contributing significantly in food web. Any effect on abundance diversity copepods climate change is likely have serious implications ecosystem functioning. Molecular studies that play a vital role assessing genetic changes under influence environmental imbalances completely lacking for this species. Here we report...