A5018610795- Gut microbiota and health
- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Urinary Tract Infections Management
- T-cell and Retrovirus Studies
- Flavonoids in Medical Research
- Cancer Research and Treatments
- Vector-Borne Animal Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Colorectal Cancer Screening and Detection
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Atherosclerosis and Cardiovascular Diseases
- Natural product bioactivities and synthesis
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Medicinal Plants and Bioactive Compounds
- Immune cells in cancer
- Natural Antidiabetic Agents Studies
- Phytochemistry and Biological Activities
- Hepatitis C virus research
- Cytokine Signaling Pathways and Interactions
Fox Chase Cancer Center
2020-2024
University Hospitals of Cleveland
2022-2023
Drexel University
2014-2022
Thomas Jefferson University
2022
Institute for Molecular Medicine
2015-2020
Abstract Neoadjuvant chemotherapy (NAC) is linked with clinical advantages in urothelial carcinoma for patients muscle-invasive bladder cancer (MIBC). Despite comprehensive research into the influence of tumor mutation expression profiles and clinicopathologic factors on response, role gut microbiome (GM) response remains poorly understood. This study examines variance GM compared healthy adults, investigates compositional differences between who respond to versus those exhibit residual...
The mechanism of dendritic cells (DCs) recruitment across the blood brain barrier (BBB) during neuroinflammation has been least explored amongst all leukocytes. For myeloid origin, while integrins function at level adhesion, importance lectins remains unknown. Here, we identified functions one C-type lectin receptor, CLEC12A, in facilitating DC binding and transmigration BBB response to CCL2 chemotaxis. To test CLEC12A an animal model multiple sclerosis (MS), administered blocking antibody...
Until recently, the properties of microbiome and mycobiome in humans its relevance to disease have largely been unexplored. While interest malignancy over past few years burgeoned with advent new technologies, no research describing composition bladder cancer has done. Deciphering metagenome aggregate genetic information can be used understand functional relationships between bacteria, fungi, cancer.The aim this project is characterize compositional range normal versus gut.An internal...
The exact molecular mechanisms regarding HTLV-1 Tax-mediated viral gene expression and CD4 T-cell transformation have yet to be fully delineated. Herein, utilizing virus-infected primary CD4+ T cells the virus-producing cell line, MT-2, we describe involvement regulation of Myocyte enhancer factor-2 (specifically MEF-2A) during course infection associated disease syndrome.Inhibition MEF-2 by shRNA its activity HDAC9 led reduced replication in correlation with a heightened ATL patients....
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little about IFN-α-regulated miRNAs in DCs. Since several are involved regulating DC functions, it important investigate whether IFN-α's DCs mediated as well. In this study, we examined miRNA expression patterns myeloid (mDCs) plasmacytoid after exposing them IFN-α. We report that IFN-α downregulates miR-221...
Abstract Treatment with neoadjuvant chemotherapy (NAC) in muscle invasive bladder cancer (MIBC) is associated clinical benefit urothelial carcinoma. While extensive research evaluating role of tumor mutational expression profiles and clinicopathologic factors into chemoresponse has been published, the gut microbiome (GM) not thoroughly evaluated. A working knowledge its effect on all forms therapy BC critical. Here we examine patients undergoing NAC. Overall, there was no significant...
Adult T-cell leukemia and lymphoma (ATLL) is an intractable neoplasia caused by a retrovirus, namely human virus type 1 (HTLV-1). Patients suffering from ATLL present poor prognosis have dearth of treatment options. In contrast to the sporadic expression viral transactivator protein Tax at 5’ promoter region long terminal repeats (LTR), HTLV-1 bZIP gene (HBZ) encoded 3’LTR (the antisense promoter) maintains its constant in cells patients. The associated with selective retroviral has been...
Abstract Flavonoids have a range of biological activities, including anti-inflammatory, anti-carcinogenic, and neuroprotective effects. Apigenin, one such flavonoid abundant in certain plants, has been employed for centuries the treatment Parkinson’s, neuralgia, asthma other diseases owing to positive health effects attributed this group compounds. Due Apigenin’s relative abundance low intrinsic toxicity amongst flavonoids, it can be considered as potential natural inflammatory disorders...
<p>Bladder cancer versus controls microbiome diversity and differences. <b>A,</b> Stacked bar plot displaying the relative abundances of predominant bacterial genera identified in patients with bladder (<i>n</i> = 142) control individuals 48). <b>B,</b> Distribution alpha indices across both cohorts, quantified using ACE, Chao1, Observed species, Pielou's evenness, Shannon, Simpson indices, error bars denoting variability within each group....
<p>Supplementary Figure 9: Microbial Composition Variability by Sex in BBN-Exposed Mice</p><p>BBN Gender Female:</p><p>A: Alpha diversity indices (ACE, Chao1, Shannon) across different time points (0, 6, 12, 16-22 weeks), showing variations microbial female mice.</p><p>B: Stacked bar chart illustrating the relative abundance of bacterial genera at each point.</p><p>C: Dot plot depicting percentage specific families points, providing a...
<p>Supplementary Figure 3: Imaging of Murine Urothelial Cancer CT urography and ultrasound images displaying bladder tumors induced by BBN in mice, shown both axial coronal views, with highlighted arrows.</p>
<p>Supplementary Figure 3: Imaging of Murine Urothelial Cancer CT urography and ultrasound images displaying bladder tumors induced by BBN in mice, shown both axial coronal views, with highlighted arrows.</p>
<p>Supplementary Figure 6: Gut Microbiome Dynamics During Neoadjuvant Chemotherapy</p><p>A: Relative abundance of major bacterial genera before, during, and after chemotherapy. Each color represents a different genus, with patient-specific changes highlighted to illustrate shifts in microbial composition over the course treatment.</p><p>B: Violin plots representing alpha diversity indices (ACE, Chao1, Observed, Pielou, Shannon, Simpson) at three stages...
<p>Supplementary Figure 2: Patient Recruitment Flow Chart Flowchart illustrating the step-by-step recruitment process of bladder cancer patients, detailing inclusion and exclusion criteria subsequent enrollment stages</p>
<p>Longitudinal microbiota analysis in a murine model exposed to BBN versus water. <b>A,</b> Shannon diversity index measures across different timepoints (0, 6, 12, 16–22 weeks) showing changes alpha mice treated with compared those given Each point represents time-specific score, providing insights into the temporal dynamics of microbial diversity. <b>B,</b> PCoA plot beta based on Weighted UniFrac distances, illustrating community variations over time between...
<p>Microbial composition variance in relation to chemotherapy response patients with bladder cancer. <b>A,</b> Proportional representation of the GM's phylogenetic showing CR versus NR NAC (Also depicted <b>C</b>). <b>B,</b> Alpha diversity metrics compared across groups, highlighting minimal statistical disparities. <b>D,</b> Weighted UniFrac PCoA depicting lack distinct microbial clustering by category....
<p>Analysis of SCFA concentrations in relation to chemotherapy response. Top: Mean plasma and stool patients with bladder cancer categorized by NAC response status (CR vs. NR), showing no significant differences. Bottom: Correlation matrices between levels bacterial genera stool, the strongest positive negative correlations marked, indicating metabolic interactions within GM context therapy response.</p>
<p>Supplementary Figure 7: Gut Microbiome Changes in Partners of Bladder Cancer Patients</p><p>A: Stacked bar chart representing the relative abundances key bacterial genera before, during, and after chemotherapy periods partners patients.</p><p>B: Principal Coordinates Analysis (PCoA) plot based on weighted UniFrac distances showing clustering microbiome samples at different stages (before, after). The ellipses represent 95% confidence intervals for each...