A5066906019- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- Fatty Acid Research and Health
- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and biosynthesis
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
On-tissue chemical derivatization combined with mass spectrometry imaging (MSI) can effectively visualize low-abundance and poorly ionizable molecules in biological tissues. Owing to the lack of an effective reaction environment on tissue surface, development direct one-step reactions is challenging. Herein, we present a two-step involving on-tissue oxidation followed by airflow-assisted desorption electrospray ionization-MSI, enabling visualization primary secondary hydroxyl-containing...
Rationale Multicellular tumor spheroids (MCTSs) that reconstitute the metabolic characteristics of in vivo tissue may facilitate discovery molecular biomarkers and effective anticancer therapies. However, little is known about how cancer cells adapt their changes complex three‐dimensional (3D) microenvironments. Here, using two‐dimensional (2D) cell model as control, phenotypes glioma U87MG multicellular were systematically investigated based on static metabolomics dynamic fluxomics...
Spatially resolved lipidomics is pivotal for detecting and interpreting lipidomes within spatial contexts using the mass spectrometry imaging (MSI) technique. However, comprehensive efficient lipid identification in MSI remains challenging. Herein, we introduce a high-coverage, database-driven approach combined with air-flow-assisted desorption electrospray ionization (AFADESI)-MSI to generate profiles across whole-body mice. Using liquid chromatography coupled tandem (LC–MS/MS), identified...