A5034082955- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Photosynthetic Processes and Mechanisms
- Biochemical and biochemical processes
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Machine Learning in Bioinformatics
- Insect Resistance and Genetics
- Fungal Biology and Applications
- Light effects on plants
- Algal biology and biofuel production
Tianjin University
2022-2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2022-2024
Shaoxing University
2023-2024
Qilu University of Technology
2020
Shandong Academy of Sciences
2019-2020
α-Farnesene, a type of acyclic sesquiterpene, is an important raw material in agriculture, aircraft fuel, and the chemical industry. In this study, we constructed efficient α-farnesene-producing yeast cell factory by combining enzyme metabolic engineering strategies. First, screened different plants for α-farnesene synthase (AFS) with best activity found that AFS from Camellia sinensis (CsAFS) exhibited most production Saccharomyces cerevisiae 4741. Second, flux mevalonate pathway was...
Sesquiterpenes comprise a diverse group of natural products with wide range applications in cosmetics, food, medicine, agriculture, and biofuels. Heterologous biosynthesis is increasingly employed for sesquiterpene production, aiming to overcome the limitations associated chemical synthesis extraction. Sesquiterpene synthases (STSs) play crucial role heterologous sesquiterpene. Under catalysis STSs, over 300 skeletons are produced through various cyclization processes (C1-C10 closure, C1-C11...
Terpene synthases (TPS) catalyze the cyclization of acyclic prenyl diphosphate precursor and are responsible for abundance natural product terpene in nature. The biosynthesis terpenoid by nonseed plant TPS is not well understood due to highly dynamic feature reaction unavailability enzyme structure. Here we discovered a class I JeST4 from Jungermannia exsertifolia elucidated its catalytically active structure complex with substrate key carbocation intermediates during catalysis. We found...
Kauralexin A1 (KA1) is a key intermediate of the kauralexin A series metabolites maize phytoalexins. However, their application severely limited by low abundance in maize. In this study, an efficient biosynthetic pathway was constructed to produce KA1 Saccharomyces cerevisiae. Also, metabolic and enzyme engineering strategies were applied construct high-titer strains, such as chassis modification, screening synthases, colocalization enzymes, multiple genomic integrations. First, precursor...
Zealexin A1 is a nonvolatile sesquiterpene phytoalexin, which not only exhibits extensive antifungal and insecticidal activities but also has the ability to enhance drought resistance of plants, thus potential applications in agricultural food fields. In this study, biosynthetic pathway zealexin was constructed
The liverwort Jungermannia exsertifolia is one of the oldest terrestrial plants and rich in structurally specific sesquiterpenes. There are several sesquiterpene synthases (STSs) with non-classical conserved motifs that have been discovered recent studies on liverworts; these aspartate bind cofactors. However, more detailed sequence information needed to clarify biochemical diversity atypical STSs. This study mined J. (JeSTSs) through transcriptome analysis using BGISEQ-500 sequencing...
<title>Abstract</title> Designing enzyme sequences to enhance product yield represents a fundamental challenge in metabolic engineering. Here, we established workflow that integrates computational predictions with efficient experimental iteration obtain outsized gains yield. Based on causal inference and examination of published datasets from previous yield-boosting engineering efforts, realized ultimately experimentally confirmed <italic>in vivo</italic> unit (yield/expression) can serve as...
Phycoerythrin (PE) is rich in Porphyridium purpureum (P. purpureum), but it varied greatly with the culture conditions and time. Therefore, exploring rules of PE synthesis accumulation capturing maximum content can effectively reduce time, so adapt to large-scale production. as a new type natural fluorescent pigment protein, widely used food, cosmetics medicine industries due its excellent fluorescence intensity, antioxidant activity, free radical scavenging, high chromaticity other...