A5065802959- Gut microbiota and health
- Probiotics and Fermented Foods
- Genetic Neurodegenerative Diseases
- Gastrointestinal motility and disorders
- Microbial Metabolites in Food Biotechnology
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- GABA and Rice Research
- RNA Research and Splicing
- Cardiomyopathy and Myosin Studies
- Digestive system and related health
- Mitochondrial Function and Pathology
- Diet and metabolism studies
Yakult Central Institute
2020-2024
The University of Tokyo
2013-2015
Abstract Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand ecology gut microbiota their symbiotic relationships with hosts, spatial distribution bacteria must be examined. Here, we analyzed composition luminal (LM) mucosa-associated (MAM) ascending descending colons rectums 10 healthy adults compared it total composition. The abundance Lachnospiraceae LM decreased along length was significantly lower than that LM. Contrastingly,...
Myotonic dystrophy type 1 (DM1) is the most common muscular in adults and as yet no cure for DM1. Here, we report potential of manumycin A a novel DM1 therapeutic reagent. caused by expansion CTG repeat. Mutant transcripts containing expanded CUG repeats lead to aberrant regulation alternative splicing. Myotonia (delayed muscle relaxation) commonly observed symptom patients splicing skeletal chloride channel (CLCN1) gene. Identification small-molecule compounds that correct attracting much...
Probiotic products have been shown to beneficial effects on human hosts, but what happens in the gastrointestinal tract after its ingestion remains unclear. Our aim was investigate changes within small intestines a single intake of fermented milk product containing probiotic. We periodically collected small-intestinal fluids from terminal ileum seven healthy subjects for up 7 h by fluid perfusion using an endoscopic retrograde bowel insertion technique. The bacterial composition clearly...
Bile acid resistance is crucial to allow probiotic strains survive in the gastrointestinal tract and exert health-promoting effects on their hosts. Our aim here was determine mechanism of this via a genetic approach by identifying genes essential for bile Lacticaseibacillus paracasei strain Shirota (LcS). We generated 4649 transposon-inserted lines L. YIT 0291, which has same genome sequence as LcS but lacks pLY101 plasmid, we screened them bile-acid-sensitive mutants. The growth 14 mutated...
ABSTRACT The viability of probiotics in the gastrointestinal tract is essential because it has an important role their health benefits. Plasticity a phenomenon associated with stress tolerance bacteria, and probiotic strains can be induced by use additives weak stress. However, some these processes are difficult to implement manufacture beverages, there few methods that easily applied. Co-culture techniques have been used produce fermented beverages may impart distinct characteristics...
Expression of chloride channel 1 (CLCN1/ClC-1) in skeletal muscle is driven by alternative splicing, a process regulated part RNA-binding protein families MBNL and CELF. Aberrant splicing CLCN1 produces many mRNAs, which were translated into inactive proteins, resulting myotonia myotonic dystrophy (DM), genetic disorder caused the expansion CTG or CCTG repeat. This increase abnormal variants containing exons 6B, 7A insertion TAG stop codon just before exon 7 leads to decrease expression...
Mononuclear phagocytic cells (MPCs) are classified into monocytes (Mos)/macrophages and dendritic (DCs) based on their functions. Cells of MPCs lineage act as immune modulators by affecting effector cells, such NK T B cells. This study aimed to investigate the effects Lacticaseibacillus paracasei strain Shirota (LcS) ingestion peripheral MPCs, particularly expression functional cell-surface molecules enhanced in healthy adults. Thus, twelve office workers consumed a fermented milk drink...